揭示了生物制备的紫花蕉皮硒纳米颗粒在抗菌、抗生物膜、抑制毒力基因(can和LuxS)、抗癌和抗氧化等方面的潜力。

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Mohamed K Y Soliman, Salem S Salem
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引用次数: 0

摘要

本研究采用一种生态可行的方法,利用菠萝皮废物(PPW)提取物生产硒纳米粒子(SeNPs),旨在实现多种医疗用途。我们的分析表明,ppw提取物是几种重要植物化学物质的重要供应商。通过XRD、FTIR、SEM、EDX、UV-Vis、HRTEM等手段对合成的SeNPs进行了全面表征,其结构呈球形,尺寸在33 ~ 73 nm之间。对SeNPs进行了额外的实验评估,以确定其在生物学应用中的适用性。研究结果表明,获得的SeNPs可以有效地对抗多种细菌,包括金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌、粪便芽孢杆菌和肺炎克雷伯菌。此外,SeNPs对MRSA和大肠杆菌均表现出抗生物膜能力,在100µg/mL时分别抑制64.8%和54.4%。与RecA基因相比,在62.5-31.25µg/mL范围内,SeNPs降低了金黄色葡萄球菌生成生物膜所需的两个必需基因cna(变化0.9倍)和大肠杆菌群体感应基因LuxS(对照为4.2倍,处理为3.4倍)的表达。SeNPs的抗氧化能力IC50值为98.3µg/mL。形成的SeNPs对HepG2恶性细胞系具有抗癌作用,IC50值为113.02µg/mL。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Uncovering the potential of biofabricated Ananas comosus peel selenium nanoparticles for antibacterial, antibiofilm, suppression of virulence genes (can and LuxS), anticancer, and antioxidant properties.

This research employed Ananas comosus (pineapple) peel waste (PPW) extract for producing selenium nanoparticles (SeNPs) using an ecologically feasible way, aimed at various medical uses. Our analysis demonstrated that the PPWextract was a significant supplier of several important phytochemicals. The synthesized SeNPs were comprehensively characterized via XRD, FTIR, SEM, EDX, UV-Vis, and HRTEM which exhibiting a spherical shape with dimensions between 33 and 73 nm. Additional experimental assessments of SeNPs were carried out to ascertain their suitability for usage in biology applications. The findings suggest that obtained SeNPs may effectively combat multiple bacteria, including S. aureus, E. coli, B. subtilis, E. faecalis, and K. pneumonia. Additionally, SeNPs exhibited antibiofilm capacity for both MRSA and E. coli with inhibition reported to be 64.8% and 54.4% at 100 µg/mL respectively. In the range of 62.5-31.25 µg/mL SeNPs reduced expression of two essential genes required for S. aureus to generate biofilms, cna (0.9 fold change), and quorum sensing gene LuxS of E. coli (4.2 folds of control to 3.4 folds of treated) in comparison to the RecA gene. The antioxidant capacity of SeNPs reported an IC50 value of 98.3 µg/mL. The formed SeNPs demonstrated anticancer efficacy in combating the HepG2 malignant cell line, with an IC50 value of 113.02 µg/mL.

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来源期刊
BMC Biotechnology
BMC Biotechnology 工程技术-生物工程与应用微生物
CiteScore
6.60
自引率
0.00%
发文量
34
审稿时长
2 months
期刊介绍: BMC Biotechnology is an open access, peer-reviewed journal that considers articles on the manipulation of biological macromolecules or organisms for use in experimental procedures, cellular and tissue engineering or in the pharmaceutical, agricultural biotechnology and allied industries.
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