使用天然质谱法定量蛋白质-聚糖相互作用。

IF 6.6 2区 化学 Q1 SPECTROSCOPY
Duong T Bui, Elena N Kitova, Ling Han, Lara K Mahal, John S Klassen
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引用次数: 0

摘要

聚糖结合蛋白(GBPs)与碳水化合物(聚糖)之间的相互作用对许多与人类健康和疾病相关的生物过程至关重要。然而,对于大多数GBPs来说,它们的聚糖相互作用组——被识别的聚糖及其特异性——是不明确的。生物相关聚糖的结构多样性及其纯化形式的有限可用性,以及它们不同的表现形式(通常是糖缀合物)和弱亲和力是阻碍全面的聚糖相互作用制图的关键挑战。原生质谱(nMS)是一种多功能、敏感和无标记的工具,用于发现GBPs -聚糖相互作用并量化它们的化学计量学和热力学参数,有望在定义GBPs的聚糖相互作用组方面发挥主导作用。在这里,我们回顾了已建立的nMS方法,以及重要的实验和仪器考虑因素,用于检测gbp -聚糖在体外的相互作用,并可靠地测量它们的化学计量学和亲和力。本文还介绍了用于高通量文库筛选的nMS方法的最新进展,包括散弹枪糖组学,以及定量GBP与糖蛋白和鞘糖脂的相互作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Quantifying Protein-Glycan Interactions Using Native Mass Spectrometry.

Interactions between glycan-binding proteins (GBPs) and carbohydrates (glycans) are essential to many biological processes relevant to human health and disease. For most GBPs, however, their glycan interactome-the repertoire of glycans recognized and their specificities-is poorly defined. The structural diversity of biologically relevant glycans and their limited availability in purified form, as well as their varied presentation, often as glycoconjugates, and weak affinities are key challenges hindering comprehensive glycan interaction mapping. Native mass spectrometry (nMS), a versatile, sensitive and label-free tool for the discovery of GBP-glycan interactions and quantifying their stoichiometry and thermodynamic parameters, is poised to play a leading role in defining the glycan interactome of GBPs. Here, we review established nMS methodologies, as well as important experimental and instrumental considerations, for detecting GBP-glycan interactions in vitro, and reliably measuring their stoichiometry and affinity. Recent advances in nMS methods for high-throughput library screening, including shotgun glycomics, and quantifying GBP interactions with glycoproteins and glycosphingolipids, are also described.

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来源期刊
Mass Spectrometry Reviews
Mass Spectrometry Reviews 物理-光谱学
CiteScore
16.30
自引率
3.00%
发文量
56
期刊介绍: The aim of the journal Mass Spectrometry Reviews is to publish well-written reviews in selected topics in the various sub-fields of mass spectrometry as a means to summarize the research that has been performed in that area, to focus attention of other researchers, to critically review the published material, and to stimulate further research in that area. The scope of the published reviews include, but are not limited to topics, such as theoretical treatments, instrumental design, ionization methods, analyzers, detectors, application to the qualitative and quantitative analysis of various compounds or elements, basic ion chemistry and structure studies, ion energetic studies, and studies on biomolecules, polymers, etc.
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