柱状微阵列羟基磷灰石的成纤维细胞形态、粘附和细胞外基质分泌。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2025-06-30 DOI:10.1021/acsabm.5c00595

Takuma Watanabe, Gerardo Martin Quindoza, Yuta Aida, Hayato Laurence Mizuno, Yasutaka Anraku, Toshiyuki Ikoma

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引用次数: 0

摘要

模拟天然牙釉质柱状结构和生物力学的仿生牙种植体已经得到了广泛的研究。然而，纤维整合，对种植体稳定性至关重要的过程，在很大程度上仍未得到充分的研究。本研究采用柱状微阵列结构研究了NIH3T3成纤维细胞与羟基磷灰石（HAp）陶瓷体的粘附和细胞外基质（ECM）分泌。扫描电镜和免疫荧光分析显示树突状成纤维细胞形态，突出物穿透并锚定在平行排列的HAp柱上。此外，还观察到ECM沿柱的浸润和纤维结构。这些发现表明，牙釉质样结构增强了纤维整合，潜在地降低了种植体失败的风险。

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Fibroblast Morphology, Adhesion, and Extracellular Matrix Secretion in Columnar Microarray Hydroxyapatite.

Biomimetic dental implants mimicking the columnar structure and biomechanics of natural enamel have been extensively studied. However, fibrointegration, a process essential for implant stability, remains largely underexplored. This study investigated the adhesion of NIH3T3 fibroblasts and extracellular matrix (ECM) secretion on hydroxyapatite (HAp) ceramic bodies with a columnar microarray architecture. Scanning electron microscopy and immunofluorescence analyses revealed dendritic fibroblast morphology, with protrusions penetrating and anchoring to parallel-aligned HAp pillars. ECM infiltration and fibrous structures along the pillars were also observed. These findings suggest that the enamel-like structure enhances fibrointegration, potentially reducing the risk of implant failure.

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.