揭示猕猴桃成熟和成熟过程中授粉的隐藏动态

IF 6.8 1区 农林科学 Q1 AGRONOMY
Marios Georgios Kollaros , Michail Michailidis , Alexandra Poulouktsi , Daniil Achilleas Pavlidis , Christina Skodra , Chrysanthi Polychroniadou , Martina Samiotaki , Katerina Karamanoli , Georgia Tanou , Athanassios Molassiotis
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引用次数: 0

摘要

人工授粉在猕猴桃栽培中越来越受到重视。然而,不同的授粉方式如何影响果实的成熟仍然知之甚少。为解决这一问题,研究了不同授粉方式(人工授粉和露天授粉)对猕猴桃果皮、胎座和种子组织的生理、代谢组学、蛋白质组学和基因表达的影响。研究了“海沃德”猕猴桃在采收成熟期和采收后在短期和长期冷藏后的成熟期。与露天授粉相比,人工授粉增加了坐果和种子数量,收获时果实大小和重量增加,支持了人工授粉在提高猕猴桃产量方面的作用。代谢组学分析表明,在人工授粉的果实中,碳主要从糖合成转向有机酸生产。组织特异性蛋白质组学分析表明,人工授粉改变了植物生长调节剂的动态,诱导了广泛的胁迫相关反应。此外,人工授粉加速了猕猴桃的成熟,乙烯产量增加,果实软化速度加快。这些变化伴随着涉及乙烯信号和细胞壁结构的基因和蛋白质表达的改变,可能会减少采后寿命。此外,人工授粉减少了关键酯类,增加了醛类,从而改变了香气挥发谱。它还降低了重要多酚的水平,特别是儿茶素、表儿茶素和原花青素B2,这与观察到的基因表达变化一致。这些发现强调了一个关键的权衡:虽然人工授粉提高了产量,但它也调节了可能影响采后果实质量的生理过程。总的来说,这项研究为授粉如何影响猕猴桃的成熟和成熟提供了新的见解,支持基于授粉的策略来提高果实产量和质量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Uncovering the hidden dynamics of pollination in kiwifruit maturity and ripening
Artificial pollination has gained increasing attention in kiwifruit cultivation; however, how different pollination methods influence fruit maturity and ripening remains poorly understood. To address this, the physiological, metabolomic, proteomic and gene expression impact of pollination methods (artificial versus open-field pollination) on the pericarp, placenta and seed tissue of Actinidia chinensis var. deliciosa A. Chev. ‘Hayward’ kiwifruit at maturity harvest and during postharvest ripening following short and long cold storage was investigated. Artificial pollination enhanced fruit set and seed number, resulting in increased fruit size and weight at harvest compared to open-field pollination, supporting its role in improving kiwifruit yield. Metabolomic analysis revealed that carbon is primarily redirected from sugar synthesis toward organic acid production in artificially pollinated fruit. Tissue-specific proteomic analysis indicated that artificial pollination alters plant growth regulator dynamics and induce extensive stress-associated responses. Moreover, artificial pollination accelerated kiwifruit ripening as evidenced by increased ethylene production and faster fruit softening. These changes were accompanied by altered expression of genes and proteins involved in ethylene signaling and cell wall structure, potentially reducing postharvest longevity. Additionally, artificial pollination decreased key esters and increased aldehydes, thus altering aroma volatile profiles. It also reduced the levels of important polyphenols, particularly catechin, epicatechin and procyanidin B2, which aligned with observed changes in gene expression. These findings highlight a critical trade-off: while artificial pollination enhances yield, it also modulates physiological processes that may compromise postharvest fruit quality. Overall, this study provides new insights into how pollination influences kiwifruit maturity and ripening, supporting pollination-based strategies to enhance both fruit yield and quality.
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来源期刊
Postharvest Biology and Technology
Postharvest Biology and Technology 农林科学-农艺学
CiteScore
12.00
自引率
11.40%
发文量
309
审稿时长
38 days
期刊介绍: The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.
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