智利北部蜱类单核细胞埃利希体病病原相关基因种的检测

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2025-06-30 DOI:10.1016/j.vprsr.2025.101309

Adriana Santodomingo , Richard Thomas , Catalina Parragué-Migone , Sebastián Muñoz-Leal , Gerardo Acosta-Jamett

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引用次数: 0

摘要

无浆菌科由专性细胞内细菌组成，其中一些是医学和兽医关注的蜱传病原体。在南美洲，对无形体科的有限研究限制了我们对其流行病学、多样性和宿主-媒介动力学的理解。对智利北部地区采集的硬蜱进行了无原体科分子调查。采用16S rRNA （rrs）、gltA和groEL位点的PCR方法对蜱进行形态学鉴定和筛选。遗传分析显示，在寄生于达尔文Phyllotis darwini啮齿动物的Ixodes若虫中检测到一个与chaffelichia -associated （ECA）分支相关的埃利希体基因种。ECA分支包括人畜共患的物种，如沙菲伊蚊和muris埃利希体亚种。euclarensis。这是与新热带sigelos蜱虫复合体相关的eca相关埃利希体基因种的第一个证据。虽然其致病性尚不清楚，但其与人畜共患物种的系统发育联系值得进一步调查。对伊蚊和野生哺乳动物的进一步监测对于阐明其分布、生态作用和公共卫生影响至关重要。

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Detection of an Ehrlichia genospecies related to agents of human monocytic ehrlichiosis in Ixodes ticks from northern Chile

The family Anaplasmataceae comprises obligate intracellular bacteria, some of which are tick-borne pathogens of medical and veterinary concern. In South America, limited research on Anaplasmataceae restricts our understanding of their epidemiology, diversity, and host-vector dynamics. We conducted a molecular survey of Anaplasmataceae on Ixodes spp. ticks collected from rodents in northern Chile. Ticks were morphologically identified and screened by PCR targeting the 16S rRNA (rrs), gltA, and groEL loci. Genetic analyses revealed an Ehrlichia genospecies related to the Ehrlichia chaffeensis-associated (ECA) clade, detected in an Ixodes nymph parasitizing a Phyllotis darwini rodent. The ECA clade includes zoonotic species such as E. chaffeensis and Ehrlichia muris subsp. euclarensis. This is the first evidence of an ECA-related Ehrlichia genospecies associated with the Neotropical Ixodes sigelos tick complex. Although its pathogenicity is unknown, its phylogenetic link to zoonotic species warrants further investigation. Additional surveillance of Ixodes spp. and wild mammals is essential to clarify its distribution, ecological role, and public health implications.

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).