{"title":"拉曼光谱和FTIR-ATR光谱技术结合化学计量学:驴奶掺假的表征和比较","authors":"Sinem Çolak","doi":"10.1002/jrs.6812","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Due to its nutritional qualities, donkey milk is a newly popular food. Because it has a high added value, it may be adulterated with using cheaper milks, like cow milk. This study has investigated a rapid method for the authentication of pure donkey milk using Raman and FTIR-ATR spectroscopy and comparison of the methods. Three preprocessing methods were applied to the spectra. The results show that donkey milk has lower fat and protein content compared with cow milk. Notably, Raman spectroscopy successfully distinguishes donkey and cow milk according to the presence and absence of β-carotene. Principal component analysis demonstrated a distinct separation between cow, adulterated donkey, and donkey. The variance value of 90.30% (PC1 = 72.76, PC2 = 17.53) is obtained from the first and second PCs for Raman data, and the variance value of 89.67% (PC1 = 65.25, PC2 = 24.41) is obtained from the first and second PCs for FTIR data. The Raman data could be used to separate donkey and cow milks, whereas the FTIR data were insufficient. It was observed that adulterated species could be separated between classes with Raman and FTIR. In the FTIR spectrum, there is a broad peak due to water, which accounts for about 87% of the milk composition, but this water peak is not included in the Raman spectrum. The results demonstrate the potential of Raman spectroscopy as a rapid and reliable method and suggest that it can be used as a nondestructive analytical tool for adulteration detection in donkey milk.</p>\n </div>","PeriodicalId":16926,"journal":{"name":"Journal of Raman Spectroscopy","volume":"56 7","pages":"598-608"},"PeriodicalIF":2.4000,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Simultaneous Raman and FTIR-ATR Spectroscopy Techniques Combined With Chemometrics: Characterization and Comparison of Donkey Milk Adulteration\",\"authors\":\"Sinem Çolak\",\"doi\":\"10.1002/jrs.6812\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>Due to its nutritional qualities, donkey milk is a newly popular food. Because it has a high added value, it may be adulterated with using cheaper milks, like cow milk. This study has investigated a rapid method for the authentication of pure donkey milk using Raman and FTIR-ATR spectroscopy and comparison of the methods. Three preprocessing methods were applied to the spectra. The results show that donkey milk has lower fat and protein content compared with cow milk. Notably, Raman spectroscopy successfully distinguishes donkey and cow milk according to the presence and absence of β-carotene. Principal component analysis demonstrated a distinct separation between cow, adulterated donkey, and donkey. The variance value of 90.30% (PC1 = 72.76, PC2 = 17.53) is obtained from the first and second PCs for Raman data, and the variance value of 89.67% (PC1 = 65.25, PC2 = 24.41) is obtained from the first and second PCs for FTIR data. The Raman data could be used to separate donkey and cow milks, whereas the FTIR data were insufficient. It was observed that adulterated species could be separated between classes with Raman and FTIR. In the FTIR spectrum, there is a broad peak due to water, which accounts for about 87% of the milk composition, but this water peak is not included in the Raman spectrum. The results demonstrate the potential of Raman spectroscopy as a rapid and reliable method and suggest that it can be used as a nondestructive analytical tool for adulteration detection in donkey milk.</p>\\n </div>\",\"PeriodicalId\":16926,\"journal\":{\"name\":\"Journal of Raman Spectroscopy\",\"volume\":\"56 7\",\"pages\":\"598-608\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2025-03-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Raman Spectroscopy\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/jrs.6812\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"SPECTROSCOPY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Raman Spectroscopy","FirstCategoryId":"92","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jrs.6812","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"SPECTROSCOPY","Score":null,"Total":0}
Simultaneous Raman and FTIR-ATR Spectroscopy Techniques Combined With Chemometrics: Characterization and Comparison of Donkey Milk Adulteration
Due to its nutritional qualities, donkey milk is a newly popular food. Because it has a high added value, it may be adulterated with using cheaper milks, like cow milk. This study has investigated a rapid method for the authentication of pure donkey milk using Raman and FTIR-ATR spectroscopy and comparison of the methods. Three preprocessing methods were applied to the spectra. The results show that donkey milk has lower fat and protein content compared with cow milk. Notably, Raman spectroscopy successfully distinguishes donkey and cow milk according to the presence and absence of β-carotene. Principal component analysis demonstrated a distinct separation between cow, adulterated donkey, and donkey. The variance value of 90.30% (PC1 = 72.76, PC2 = 17.53) is obtained from the first and second PCs for Raman data, and the variance value of 89.67% (PC1 = 65.25, PC2 = 24.41) is obtained from the first and second PCs for FTIR data. The Raman data could be used to separate donkey and cow milks, whereas the FTIR data were insufficient. It was observed that adulterated species could be separated between classes with Raman and FTIR. In the FTIR spectrum, there is a broad peak due to water, which accounts for about 87% of the milk composition, but this water peak is not included in the Raman spectrum. The results demonstrate the potential of Raman spectroscopy as a rapid and reliable method and suggest that it can be used as a nondestructive analytical tool for adulteration detection in donkey milk.
期刊介绍:
The Journal of Raman Spectroscopy is an international journal dedicated to the publication of original research at the cutting edge of all areas of science and technology related to Raman spectroscopy. The journal seeks to be the central forum for documenting the evolution of the broadly-defined field of Raman spectroscopy that includes an increasing number of rapidly developing techniques and an ever-widening array of interdisciplinary applications.
Such topics include time-resolved, coherent and non-linear Raman spectroscopies, nanostructure-based surface-enhanced and tip-enhanced Raman spectroscopies of molecules, resonance Raman to investigate the structure-function relationships and dynamics of biological molecules, linear and nonlinear Raman imaging and microscopy, biomedical applications of Raman, theoretical formalism and advances in quantum computational methodology of all forms of Raman scattering, Raman spectroscopy in archaeology and art, advances in remote Raman sensing and industrial applications, and Raman optical activity of all classes of chiral molecules.