低活力精子中ST8SIA4异常甲基化：全基因组鉴定方法

IF 2 4区医学 Q2 OBSTETRICS & GYNECOLOGY

Taiwanese Journal of Obstetrics & Gynecology Pub Date : 2025-07-01 DOI:10.1016/j.tjog.2025.02.005

Chia-Ching Chang , Li-Chun Liu , Yu-Ting Chou , Chia-Wei Chen , Chih-Chi Guo , Chying-Chyuan Chan , Ta-Jen Lin , Ching-Hui Chen , Ming-Tzeung Chung

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引用次数: 0

摘要

目的鉴定低活力精子异常甲基化基因，并验证候选基因ST8 α -n -乙酰神经胺α -2,8-唾液基转移酶4 （ST8SIA4）在低活力精子中的DNA甲基化水平。材料和方法使用可测量850,000个CpG位点甲基化的MethylationEPIC Array和pooled DNA策略研究了不同活力精子的全基因组甲基化谱。采用70份精液样本，包括52份正常精子样本和18份弱精子样本，检测ST8SIA4基因的DNA甲基化水平。结果利用DAVID生物信息学资源中的基因功能分类，我们发现弱精子症样本中异常甲基化的基因主要与转录调控、金属结合、微管、减数分裂、棕榈酸盐、锌和发育蛋白相关。有趣的是，这些糖基转移酶相关基因在弱精子症中都是低甲基化的，除了ST8SIA4。结论低活力精子中ST8SIA4启动子甲基化水平显著高于正常对照。

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Abnormal methylation of ST8SIA4 in low-motility sperm: A genome-wide identification approach

Objective

To identify abnormally methylated genes associated with low motility sperm and to validate the DNA methylation level of our candidate gene ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 (ST8SIA4) in low-motility sperm.

Materials and methods

The MethylationEPIC Array which can measure methylation at >850,000 CpG sites and a pooled DNA strategy were used to investigate the genome-wide methylation profiles in sperm with different motility. A total of seventy semen samples, including 52 normozoospermic controls and 18 asthenozoospermic samples, were used to examine the quantitative DNA methylation level of ST8SIA4 gene.

Results

Using the gene function classification in the DAVID Bioinformatics Resources, we found that the abnormally methylated genes in the asthenozoospermia samples were predominantly associated with transcription regulation, metal binding, microtubules, meiosis, palmitate, zinc, and developmental proteins. Interestingly, these glycosyltransferase-associated genes were all hypomethylated in asthenozoospermia, except for ST8SIA4.

Conclusion

The promoter methylation level of ST8SIA4 was significantly higher in sperm samples with low motility compared with normal control samples.

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来源期刊

Taiwanese Journal of Obstetrics & Gynecology OBSTETRICS & GYNECOLOGY-

CiteScore

3.60

自引率

23.80%

发文量

207

审稿时长

4-8 weeks

期刊介绍： Taiwanese Journal of Obstetrics and Gynecology is a peer-reviewed journal and open access publishing editorials, reviews, original articles, short communications, case reports, research letters, correspondence and letters to the editor in the field of obstetrics and gynecology. The aims of the journal are to: 1.Publish cutting-edge, innovative and topical research that addresses screening, diagnosis, management and care in women''s health 2.Deliver evidence-based information 3.Promote the sharing of clinical experience 4.Address women-related health promotion The journal provides comprehensive coverage of topics in obstetrics & gynecology and women''s health including maternal-fetal medicine, reproductive endocrinology/infertility, and gynecologic oncology. Taiwan Association of Obstetrics and Gynecology.