CXCR4在结直肠癌中的表达与肿瘤分期的关系

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-06-20 DOI:10.1016/j.genrep.2025.102281

Fang Cao , Yang Zhang , Jianhao Xu , Jiarui Min , Jihao Su , Zhe Chen , Jingfeng Gu , Zijie Xu , Song Xu

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引用次数: 0

摘要

结直肠癌（CRC）仍然是全球癌症相关死亡的主要原因，其发病率呈上升趋势，特别是在中国等发展中国家。这一趋势强调了在癌症治疗中迫切需要新的分子靶点。本研究探讨C-X-C趋化因子受体4型（CXCR4）在结直肠癌组织中与邻近非肿瘤组织的表达，评估其诊断潜力及其与肿瘤分期和临床结局的关系。利用癌症基因组图谱（TCGA）的数据和180例结直肠癌患者的临床样本，我们进行了包括生物信息学、免疫组织化学、定量PCR和Western blotting在内的综合分析。我们的研究结果显示，CXCR4在结直肠癌组织中显著过表达，表达水平与T分期和pTNM分期呈正相关，并与肿瘤局部侵袭和淋巴结转移相关。然而，我们发现CXCR4水平与总体生存率或无病生存率之间没有显著相关性。通过受试者工作特征（ROC）曲线分析进一步验证CXCR4的诊断价值。这些发现表明，CXCR4在结直肠癌中显著上调，并与肿瘤进展相关，提示其可能具有作为结直肠癌诊断和治疗靶点的潜力。然而，需要进一步的研究来评估其作为生物标志物的预测能力和适用性。

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CXCR4 expression in colorectal cancer and tumor stage correlation

Colorectal cancer (CRC) remains a leading cause of cancer-related mortality worldwide, with incidence rates on the rise, particularly in developing countries such as China. This trend underscores the urgent need for novel molecular targets in cancer treatment. This study investigates the expression of C-X-C chemokine receptor type 4 (CXCR4) in CRC tissues compared to adjacent non-tumor tissues, evaluating its diagnostic potential and its relationship with tumor staging and clinical outcomes. Utilizing data from The Cancer Genome Atlas (TCGA) and clinical samples from 180 CRC patients, we conducted comprehensive analyses that included bioinformatics, immunohistochemistry, quantitative PCR, and Western blotting. Our results reveal that CXCR4 is significantly overexpressed in CRC tissues, with expression levels positively correlating with T stage and pTNM stage, as well as being associated with local tumor invasion and lymph node metastasis. However, we found no significant correlation between CXCR4 levels and overall or disease-free survival. The diagnostic utility of CXCR4 was further validated through receiver operating characteristic (ROC) curve analysis. These findings indicate that CXCR4 is markedly upregulated in CRC and correlates with tumor progression, suggesting it may have potential as a diagnostic and therapeutic target in colorectal cancer. However, further research is needed to evaluate its predictive power and applicability as a biomarker.

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.