真核细胞DNA复制起源许可机制

IF 10.1 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nature Structural & Molecular Biology Pub Date : 2025-06-30 DOI:10.1038/s41594-025-01587-5

Bruce Stillman, John F. X. Diffley, Janet H. Iwasa

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引用次数: 0

摘要

真核细胞的DNA复制始于细胞周期G1期染色体上许多位点的复制前复合物（pre-RCs）的组装。Pre-RCs允许每个染色体在S期进行复制，并标记DNA复制的起源。在这篇综述中，我们讨论了最近的研究结果，确定了起源识别复合物（ORC）、Cdc6和与Cdt1结合的Mcm2-Mcm7 （Mcm2-7）六聚体介导的起源识别和预rc组装的机制。我们还提供了全面的视频，展示了预rc组装的多种机制，并比较了人类和酿酒酵母细胞中涉及的复合物的结构。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Mechanisms for licensing origins of DNA replication in eukaryotic cells

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Mechanisms for licensing origins of DNA replication in eukaryotic cells

The initiation of DNA replication in eukaryotic cells begins with the assembly of pre-replicative complexes (pre-RCs) at many sites along each chromosome during the G1 phase of the cell cycle. Pre-RCs license each chromosome for duplication during S phase and mark the origins of DNA replication. In this Review, we discuss and contextualize recent findings identifying the mechanisms of origin recognition and pre-RC assembly mediated by the origin recognition complex (ORC), Cdc6 and the Mcm2–Mcm7 (Mcm2-7) hexamer bound to Cdt1. We also present comprehensive videos that demonstrate the multiple mechanisms for pre-RC assembly and compare the structures of the complexes involved in human and Saccharomyces cerevisiae cells. Here, the authors comprehensively discuss the different mechanisms for origin licensing in yeast and human cells. Importantly, they present intuitive videos illustrating such mechanisms with unprecedented detail.

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来源期刊

Nature Structural & Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-BIOPHYSICS

CiteScore

22.00

自引率

1.80%

发文量

160

审稿时长

3-8 weeks

期刊介绍： Nature Structural & Molecular Biology is a comprehensive platform that combines structural and molecular research. Our journal focuses on exploring the functional and mechanistic aspects of biological processes, emphasizing how molecular components collaborate to achieve a particular function. While structural data can shed light on these insights, our publication does not require them as a prerequisite.