免疫层析试验在富含肉汤的直肠拭子标本中的验证

IF 3 4区 医学 Q2 INFECTIOUS DISEASES
Camila Loredana P.A.M. Bezerra , Barbara de Almeida Lessa Castro , Camila Rizek , Sania Alves dos Santos , Juliana Januário Gaudereto , Ana Paula Cury , Natashia Reese , Fernanda C. Lessa , Susan Bollinger , Matias Chiarastelli Salomão , ICT validation Study Group
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引用次数: 0

摘要

产碳青霉烯酶肠杆菌(CPE)的快速检测对于告知感染预防和控制行动以遏制抗微生物药物耐药性的传播至关重要。免疫层析测试(ict)提供了一种快速且具有成本效益的分子方法替代方法,但通常是为细菌分离物而不是直接临床标本设计的。我们开发了一种使用O.K.N.V.I. resistance -5 ICT (Coris Bioconcept, Gembloux, Belgium)检测富含肉汤的模拟直肠棉签中KPC、NDM、OXA-48、VIM和IMP碳青霉烯酶的方案。将35株具有良好特征的产碳青霉烯酶分离株接种到10%的粪便基质中制备模拟棉签。拭子在脑-心灌注(BHI)肉汤中(含和不含10 μg美罗培南盘),37℃孵育4和6 h。孵育后,肉汤离心,用ICT检测微球。计算每种方法(含/不含美罗培南圆盘和潜伏期)的灵敏度、特异性和准确性。棉签在不含美罗培南的BHI肉汤中孵育6 h,获得最佳性能,对所有五种酶的敏感性、特异性和准确性均为100%。用美罗培南孵育或更短的孵育时间导致较低的敏感性,对酶的敏感性从0%到100%不等。开发的方案能够在6小时内直接从富含肉汤的直肠拭子中快速准确地检测五种常见的碳青霉烯酶。该方法提供了一种基于培养和分子技术的实用替代方法,通过及时识别CPE,有可能加强感染控制措施。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Validation of immunochromatographic test in broth-enriched rectal swab specimens
Rapid detection of Carbapenemase-Producing Enterobacterales (CPE) is essential for informing infection prevention and control actions to curb the spread of antimicrobial resistance. Immunochromatographic Tests (ICTs) offer a quick and cost-effective alternative to molecular methods but are typically designed for bacterial isolates rather than direct clinical specimens. We developed a protocol using the O.K.N.V.I. RESIST-5 ICT (Coris Bioconcept, Gembloux, Belgium) to detect KPC, NDM, OXA-48, VIM, and IMP carbapenemases from broth-enriched mock rectal swabs. A total of 35 well-characterized carbapenemase-producing isolates were inoculated into a 10 % stool matrix to create mock swabs. Swabs were incubated in Brain-Heart Infusion (BHI) broth, with and without a 10 μg meropenem disk, at 37 °C for 4 and 6 h. After incubation, broths were centrifuged, and pellets were tested using the ICT. Sensitivity, specificity, and accuracy were calculated for each method (with/without meropenem disk and incubation period). Optimal performance was achieved with swabs incubated in BHI broth without meropenem for 6 h, showing 100 % sensitivity, specificity, and accuracy for all the five enzymes tested. Incubation with meropenem or shorter incubation times resulted in lower sensitivities, with per-enzyme sensitivities ranging from 0 % to 100 %. The developed protocol enables rapid and accurate detection of five common carbapenemases directly from broth-enriched rectal swabs within 6 h. This method offers a practical alternative to culture-based and molecular techniques, potentially enhancing infection control measures through timely identification of CPE.
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来源期刊
CiteScore
5.50
自引率
0.00%
发文量
925
审稿时长
41 days
期刊介绍: The Brazilian Journal of Infectious Diseases is the official publication of the Brazilian Society of Infectious Diseases (SBI). It aims to publish relevant articles in the broadest sense on all aspects of microbiology, infectious diseases and immune response to infectious agents. The BJID is a bimonthly publication and one of the most influential journals in its field in Brazil and Latin America with a high impact factor, since its inception it has garnered a growing share of the publishing market.
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