抗菌复合树脂对高龋风险儿童唾液pH动态影响的离体研究

IF 4.8 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of dentistry Pub Date : 2025-06-25 DOI:10.1016/j.jdent.2025.105933

Dora Schachter , Nouran Aboraya-Khalayla , Omer Enoch , Sigalit Blumer , Ervin I Weiss , Michal Dekel Steinkeller

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引用次数: 0

摘要

目的：乳牙复合树脂修复体经常因继发性龋齿而失败，继发性龋齿是由牙齿修复界面细菌定植和酸产生引起的。本研究旨在评估含有季铵二氧化硅（QASi）的复合树脂（以其抗菌特性而闻名）与传统修复剂相比，是否可以减少细菌酸化并降低高龋风险儿童继发性龋的风险。方法：将34名高龋风险儿童的唾液样本（10 µL唾液，~ 10⁶细菌）直接体外应用于三种修复材料：(i) Infinix with 1.5% QASi (Nobio), (ii) Filtek Supreme （3M）和（iii）汞合金（Silmet）。样品与添加2.5%葡萄糖的生长培养基在37 °C下孵育24小时。通过浊度和颜色变化每20分钟测量一次细菌生长和pH值下降。对照组包括单独的唾液和空白的聚苯乙烯表面。结果：重复测量方差分析显示，Infinix的pH值下降最慢，其次是汞合金，而Filtek Supreme的pH值下降与对照组相匹配(结论：与传统修复剂相比，Infinix复合树脂对高龋风险儿童唾液细菌的pH值下降明显较慢。临床意义：含季铵硅（QASi）填料的复合树脂能有效抑制细菌生长和酸的产生，有助于预防继发性龋齿和牙釉质脱矿。这些生物活性材料为提高修复体的寿命提供了一个有希望的解决方案，特别是在高龋风险的儿科患者中。

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Impact of antibacterial composite resin on salivary pH dynamics in high-caries-risk children: An ex-vivo study

Objectives

Composite resin restorations in primary teeth frequently fail due to secondary caries, which result from bacterial colonization and acid production at the tooth–restoration interface. This study aimed to evaluate whether composite resins containing quaternary ammonium silica (QASi), known for their antibacterial properties, can reduce bacterial acidification and lower the risk of secondary caries in high-caries-risk children compared to conventional restoratives.

Methods

Saliva samples (10 µL saliva, ∼10⁶ bacteria) from 34 high-caries-risk children were applied ex vivo directly to three restorative materials: (i) Infinix with 1.5% QASi (Nobio), (ii) Filtek Supreme (3 M), and (iii) amalgam (Silmet). Samples were incubated with growth media supplemented with 2.5% dextrose at 37 °C for 24 hours. Bacterial growth and pH decrease were measured, by turbidity and colour change, every 20 minutes. Controls included saliva alone and a blank polystyrene surface.

Results

Repeated-measures ANOVA showed Infinix had the slowest pH decline, followed by amalgam, while Filtek Supreme’s pH drop matched the control (p<0.001). Infinix also exhibited the lowest bacterial growth rate (p<0.001).

Conclusion

Saliva bacteria from high-caries-risk children exhibit significantly slower pH decrease on Infinix composite resins compared to conventional restoratives.

Clinical significance

Composite resins with quaternary ammonium silica (QASi) fillers effectively inhibit bacterial growth and acid production, helping to prevent secondary caries and enamel demineralization. These bioactive materials offer a promising solution for improving the longevity of restorations, especially in high-caries-risk pediatric patients.

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来源期刊

Journal of dentistry 医学-牙科与口腔外科

CiteScore

7.30

自引率

11.40%

发文量

349

审稿时长

35 days

期刊介绍： The Journal of Dentistry has an open access mirror journal The Journal of Dentistry: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal of Dentistry is the leading international dental journal within the field of Restorative Dentistry. Placing an emphasis on publishing novel and high-quality research papers, the Journal aims to influence the practice of dentistry at clinician, research, industry and policy-maker level on an international basis. Topics covered include the management of dental disease, periodontology, endodontology, operative dentistry, fixed and removable prosthodontics, dental biomaterials science, long-term clinical trials including epidemiology and oral health, technology transfer of new scientific instrumentation or procedures, as well as clinically relevant oral biology and translational research. The Journal of Dentistry will publish original scientific research papers including short communications. It is also interested in publishing review articles and leaders in themed areas which will be linked to new scientific research. Conference proceedings are also welcome and expressions of interest should be communicated to the Editor.