Lang Qiu, Jia-Yi Wang, Dan-Qi Li, Xue-Gui Liu, Pin-Yi Gao
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Study on the synthesis, structural characterization, and anti-hepatoma cell proliferation effects of sulfated alfalfa polysaccharides.
A water-soluble polysaccharide (APS) from Medicago Sativa L. (alfalfa) stems was sulfated via the chlorosulfonic acid (CSA)-pyridine (Pyr) method under three conditions, yielding SAPS-1, SAPS-2, and SAPS-3. Structural characterization (HPGPC, NMR, FT-IR, SEM) confirmed successful sulfation and increased water solubility. SAPSs 1-3 exhibited superior scavenging of DPPH, ABTS, and OH radicals compared to APS. Both APS and SAPSs 1-3 inhibited hepatoma cell (HepG2, Hep3B) proliferation in vitro. Cell cycle analysis showed APS-induced G0/G1 arrest, while SAPS-3 exhibited biphasic regulation by maintaining G0/G1 arrest and stimulating S-phase accumulation. The CSA-Pyr technique enhanced the inhibitory effects, offering promise for developing bioactive compounds from alfalfa biomass.
期刊介绍：
The Journal of Asian Natural Products Research (JANPR) publishes chemical and pharmaceutical studies in the English language in the field of natural product research on Asian ethnic medicine. The journal publishes work from scientists in Asian countries, e.g. China, Japan, Korea and India, including contributions from other countries concerning natural products of Asia. The journal is chemistry-orientated. Major fields covered are: isolation and structural elucidation of natural constituents (including those for non-medical uses), synthesis and transformation (including biosynthesis and biotransformation) of natural products, pharmacognosy, and allied topics. Biological evaluation of crude extracts are acceptable only as supporting data for pure isolates with well-characterized structures.
All published research articles in this journal have undergone rigorous peer review, based on initial editor screening and anonymized refereeing by at least two expert referees.
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