Raied Abou Kubaa, Ashrafou Ouro-Djobo, Kristian A Stevens, Olufemi J Alabi, Maher Al Rwahnih
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Genome characterization of prunus maculavirus 1 (PrMcV-1), a novel member of the genus maculavirus identified in prunus spp.
A novel virus with a (+)ssRNA genome was identified in a symptomless peach tree by high-throughput sequencing. The complete RACE-verified genome of the virus, tentatively named "Prunus maculavirus 1" (PrMcV-1; GenBank accession number PV231830), is 6,664 nucleotides (nt) long, excluding the poly(A) tail, and contains two open reading frames (ORFs). ORF1 (nt 64-5895) encodes a large replication-associated polyprotein, which contains conserved domains associated with methyltransferase (Mtr), papain-like protease (Pro), helicase (Hel), and RNA-dependent RNA polymerase (RdRp). ORF2 (nt 5571-6478) partially overlaps ORF1 and encodes the capsid protein (CP). Phylogenetic analysis based on both ORFs placed PrMcV-1 in a distinct clade within the genus Maculavirus of the family Tymoviridae. Experimental evidence showed that PrMcV-1 is graft-transmissible. This study expands the known host range of maculaviruses to include Prunus species.
期刊介绍：
Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.
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