{"title":"基于Nrf-2/HO-1信号通路探讨白芷苷对肾缺血再灌注损伤的保护作用及调控机制","authors":"Qixun Luo , Zunwei Zhu , Lisong Wan","doi":"10.1016/j.transproceed.2025.05.028","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>To investigate and validate the protective role of plumbagin (PL) in renal ischemia-reperfusion injury (IRI) by mediating the Nrf2/HO-1 pathway and reducing renal function damage, thereby providing a theoretical basis for the application of PL in the prevention of renal IRI.</div></div><div><h3>Methods</h3><div>Twenty-four healthy adult male BALB/c mice were randomly divided into four groups: the sham group, the IR model group (IRI group), the IRI + PL 10 mg/kg group, and the IRI + PL 10 mg/kg + ML385 (30 mg/kg) group. Four groups of mice were given drugs 2 weeks before modeling, for 2 consecutive weeks (once a day), and the modeling was started 1 hour after the administration on the 14th day. In the sham operation group, only the renal vessels were isolated without clamping ischemia, and in the other groups, minimally invasive artery clamps were used to establish the mouse RIRI injury model. Serum creatinine (Scr), blood urea nitrogen (BUN), interleukin-6 (IL-6), myeloperoxidase (MPO), serum superoxide dismutase (SOD), GSH-Px, malondialdehyde (MDA), and inflammatory levels of mice in each group were detected and analyzed; pathological examination of renal tissue injury; and the expressions of Nrf-2 and HO-1 were detected by Western blotting.</div></div><div><h3>Results</h3><div>Compared with the sham group, the IRI group showed elevated Scr, BUN, IL-6, MDA, and MPO levels, reduced SOD and GSH-Px activities, severe renal injury, and decreased Nrf-2/HO-1 expression. PL administration reduced renal injury, decreased Scr, BUN, IL-6, MDA, and MPO levels, increased SOD and GSH-Px levels, and increased Nrf-2/HO-1 expression, as confirmed by pathology and Western blotting.</div></div><div><h3>Conclusions</h3><div>PL can reduce the level of oxidative stress and the release of inflammatory factors by regulating the Nrf-2/HO-1 signaling pathway, improve renal injury induced by ischemia/reperfusion in mice, and play a renoprotective role.</div></div>","PeriodicalId":23246,"journal":{"name":"Transplantation proceedings","volume":"57 6","pages":"Pages 1180-1186"},"PeriodicalIF":0.8000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Exploring the Protective Effect and Regulatory Mechanism of Plumbagin on Renal Ischemia-Reperfusion Injury Based on the Nrf-2/HO-1 Signaling Pathway\",\"authors\":\"Qixun Luo , Zunwei Zhu , Lisong Wan\",\"doi\":\"10.1016/j.transproceed.2025.05.028\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><div>To investigate and validate the protective role of plumbagin (PL) in renal ischemia-reperfusion injury (IRI) by mediating the Nrf2/HO-1 pathway and reducing renal function damage, thereby providing a theoretical basis for the application of PL in the prevention of renal IRI.</div></div><div><h3>Methods</h3><div>Twenty-four healthy adult male BALB/c mice were randomly divided into four groups: the sham group, the IR model group (IRI group), the IRI + PL 10 mg/kg group, and the IRI + PL 10 mg/kg + ML385 (30 mg/kg) group. Four groups of mice were given drugs 2 weeks before modeling, for 2 consecutive weeks (once a day), and the modeling was started 1 hour after the administration on the 14th day. In the sham operation group, only the renal vessels were isolated without clamping ischemia, and in the other groups, minimally invasive artery clamps were used to establish the mouse RIRI injury model. Serum creatinine (Scr), blood urea nitrogen (BUN), interleukin-6 (IL-6), myeloperoxidase (MPO), serum superoxide dismutase (SOD), GSH-Px, malondialdehyde (MDA), and inflammatory levels of mice in each group were detected and analyzed; pathological examination of renal tissue injury; and the expressions of Nrf-2 and HO-1 were detected by Western blotting.</div></div><div><h3>Results</h3><div>Compared with the sham group, the IRI group showed elevated Scr, BUN, IL-6, MDA, and MPO levels, reduced SOD and GSH-Px activities, severe renal injury, and decreased Nrf-2/HO-1 expression. PL administration reduced renal injury, decreased Scr, BUN, IL-6, MDA, and MPO levels, increased SOD and GSH-Px levels, and increased Nrf-2/HO-1 expression, as confirmed by pathology and Western blotting.</div></div><div><h3>Conclusions</h3><div>PL can reduce the level of oxidative stress and the release of inflammatory factors by regulating the Nrf-2/HO-1 signaling pathway, improve renal injury induced by ischemia/reperfusion in mice, and play a renoprotective role.</div></div>\",\"PeriodicalId\":23246,\"journal\":{\"name\":\"Transplantation proceedings\",\"volume\":\"57 6\",\"pages\":\"Pages 1180-1186\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2025-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Transplantation proceedings\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0041134525002842\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Transplantation proceedings","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0041134525002842","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
Exploring the Protective Effect and Regulatory Mechanism of Plumbagin on Renal Ischemia-Reperfusion Injury Based on the Nrf-2/HO-1 Signaling Pathway
Objective
To investigate and validate the protective role of plumbagin (PL) in renal ischemia-reperfusion injury (IRI) by mediating the Nrf2/HO-1 pathway and reducing renal function damage, thereby providing a theoretical basis for the application of PL in the prevention of renal IRI.
Methods
Twenty-four healthy adult male BALB/c mice were randomly divided into four groups: the sham group, the IR model group (IRI group), the IRI + PL 10 mg/kg group, and the IRI + PL 10 mg/kg + ML385 (30 mg/kg) group. Four groups of mice were given drugs 2 weeks before modeling, for 2 consecutive weeks (once a day), and the modeling was started 1 hour after the administration on the 14th day. In the sham operation group, only the renal vessels were isolated without clamping ischemia, and in the other groups, minimally invasive artery clamps were used to establish the mouse RIRI injury model. Serum creatinine (Scr), blood urea nitrogen (BUN), interleukin-6 (IL-6), myeloperoxidase (MPO), serum superoxide dismutase (SOD), GSH-Px, malondialdehyde (MDA), and inflammatory levels of mice in each group were detected and analyzed; pathological examination of renal tissue injury; and the expressions of Nrf-2 and HO-1 were detected by Western blotting.
Results
Compared with the sham group, the IRI group showed elevated Scr, BUN, IL-6, MDA, and MPO levels, reduced SOD and GSH-Px activities, severe renal injury, and decreased Nrf-2/HO-1 expression. PL administration reduced renal injury, decreased Scr, BUN, IL-6, MDA, and MPO levels, increased SOD and GSH-Px levels, and increased Nrf-2/HO-1 expression, as confirmed by pathology and Western blotting.
Conclusions
PL can reduce the level of oxidative stress and the release of inflammatory factors by regulating the Nrf-2/HO-1 signaling pathway, improve renal injury induced by ischemia/reperfusion in mice, and play a renoprotective role.
期刊介绍:
Transplantation Proceedings publishes several different categories of manuscripts, all of which undergo extensive peer review by recognized authorities in the field prior to their acceptance for publication.
The first type of manuscripts consists of sets of papers providing an in-depth expression of the current state of the art in various rapidly developing components of world transplantation biology and medicine. These manuscripts emanate from congresses of the affiliated transplantation societies, from Symposia sponsored by the Societies, as well as special Conferences and Workshops covering related topics.
Transplantation Proceedings also publishes several special sections including publication of Clinical Transplantation Proceedings, being rapid original contributions of preclinical and clinical experiences. These manuscripts undergo review by members of the Editorial Board.
Original basic or clinical science articles, clinical trials and case studies can be submitted to the journal?s open access companion title Transplantation Reports.