施肥改变指示物种作为评价与玉米籽粒产量有关的农业实践的生物指标。

IF 4.2 2区 生物学 Q2 MICROBIOLOGY
Guoqiang Li, Jiaqing Liu, Wenya Zhang, Jvshui Hu, Peng Shi, Gehong Wei
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引用次数: 0

摘要

多样化的农业实践通过改变施肥、耕作强度和种植模式、改变土壤性质和微生物组合来重新配置农业生态系统服务。然而,微生物群落作为土壤健康和生产力的关键生物指标,对农业干扰作出反应,多种做法对生产力相关指标物种的影响需要进一步验证。本研究采用16S和ITS扩增子测序技术,通过田间试验研究了不同施肥方式对土壤性质、玉米产量和微生物群落的影响。在每个处理中,我们评估了与耕作方式和土壤生产力相关的指标物种之间的相关性。结果表明,施肥显著改变了土壤性质,提高了玉米产量23.9%,重塑了细菌和真菌群落结构,细菌丰富度提高了23%,真菌丰富度和Shannon指数分别降低了15%和20%。此外,在施肥和不施肥的情况下,耕作方式对微生物群落和粮食产量都有显著影响,尽管对土壤性质有轻微影响。每种施肥处理都鉴定出不同的细菌和真菌指示种:未施肥土壤中有21种优势细菌指示种(如芽孢杆菌、根瘤菌、链霉菌)和8种真菌指示种(如隐球菌、赤霉素、四环菌);施肥土壤中含有24种优势细菌指标(如梭菌、梭菌、乳杆菌)和6种真菌指标(如赤霉素、枝孢杆菌、Mortierella)。值得注意的是,特定指示属的丰度(例如,细菌:拟杆菌、Gemmatirosa、Iamia、溶杆菌、普雷沃氏菌、葡萄球菌、苏特氏菌;真菌:球囊菌,Fusicolla在未施肥的土壤;细菌:定辉杆菌、Haliangium、Kribbella、Rhodomicrobium、Terrimonas;真菌(Pulvinula)与粮食产量呈正相关。这些结果表明,施肥重塑了与玉米生产力相关的微生物指示物种的组成。因此,针对不同施肥策略量身定制的微生物指标组合对于评估作物生产力和评估农业实践影响至关重要。因此,监测这些指标物种可以快速评估土壤肥力变化,为施肥管理提供指导。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Fertilization Alters Indicator Species Serving as Bioindicators for Evaluating Agricultural Practices Related to Maize Grain Yield.

Diversified agricultural practices reconfigure agroecosystem services by modifying fertilization, tillage intensities, and cropping patterns, altering soil properties and microbial assemblages. However, microbial communities, as critical bioindicators of soil health and productivity, respond to agricultural disturbances, and the effects of multiple practices on productivity-associated indicator species require further validation. Using 16S and ITS amplicon sequencing, this study employed a field experiment to investigate the effects of agricultural practices on soil properties, maize productivity, and microbial communities under two fertilization treatments. Within each treatment, we assessed correlations between indicator species associated with cropping-tillage practices and soil productivity. Results showed that fertilization significantly altered soil properties, increased maize grain yield by 23.9%, and reshaped bacterial and fungal community structures, increasing bacterial richness by 23% but reducing fungal richness and Shannon index by 15% and 20%, respectively. Furthermore, cropping-tillage practices significantly affected microbial communities and grain yields in both fertilized and unfertilized treatments despite a slight influence on soil properties. Distinct sets of bacterial and fungal indicator species were identified for each fertilization treatment: unfertilized soils harbored 21 dominant bacterial indicator species (e.g., Bacillus, Rhizobium, Streptomyces) and 8 fungal indicators (e.g., Cryptococcus, Gibberella, Tetracladium); fertilized soils contained 24 dominant bacterial indicators (e.g., Fusobacterium, Clostridium, Lactobacillus) and 6 fungal indicators (e.g., Gibberella, Cladosporium, Mortierella). Notably, abundances of specific indicator genera (e.g., bacteria: Bacteroides, Gemmatirosa, Iamia, Lysobacter, Prevotella, Staphylococcus, Sutterella; fungi: Glomus, Fusicolla in unfertilized soil; bacteria: Dinghuibacter, Haliangium, Kribbella, Rhodomicrobium, Terrimonas; fungi: Pulvinula in fertilized soil) correlated positively with grain yields. These findings demonstrate that fertilization reshapes the composition of microbial indicator species significantly associated with maize productivity. Tailored microbial indicator assemblages specific to distinct fertilization strategies are therefore essential for evaluating crop productivity and assessing agricultural practice impacts. Consequently, monitoring these indicator species enables rapid assessment of soil fertility changes, offering guidance for fertilization management.

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来源期刊
Microorganisms
Microorganisms Medicine-Microbiology (medical)
CiteScore
7.40
自引率
6.70%
发文量
2168
审稿时长
20.03 days
期刊介绍: Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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