{"title":"低强度高频振动载荷下种植体成骨细胞分化的体外研究。","authors":"Sachin Chaware, Chetan Kakade, Siddhesh Borse","doi":"10.4103/ijdr.ijdr_125_24","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>The present study aimed to assess the microscopic effects of low-magnitude high frequency on dental implant surfaces using MG-63 cells and vertical vibration loading.</p><p><strong>Materials and methods: </strong>24 dental implants wrapped with collagen membranes were placed in a 12-well plate filled with MG-63 cell culture. A vibration device was constructed using 3D printing technology and employed a vertical vibration system. Three vibration cycles were utilized, including frequencies of 30 Hz, 60 Hz, and 90 Hz. Each cycle had durations of 1, 5, and 10 min/h, with a total duration of 6 h. The control group underwent no vibrational loading. After 24 h, analysis was conducted using an inverted microscope and a spectrophotometer. Alkaline phosphatase (ALP) expression was assessed using an ALP Kit.</p><p><strong>Results: </strong>The study yielded highly significant results (P < 0.001). cell activity was found to be highest with 30 Hz vibration loading compared to both 60 Hz and 90 Hz (P < 0.001). Among the various durations tested, the 30 Hz vibration with a 5-min cycle per hour, totalling 6 h, exhibited the highest cell activity. Additionally, ALP expression was most pronounced with 30 Hz vibrational loading. The cells displayed a dark green appearance surrounded by a pale green extracellular matrix.</p><p><strong>Conclusion: </strong>The highest cell activity and ALP expression were reported with the 30 Hz vibrational loading lasting 5 min/h, over a total duration of 6 h. However, a decline in cell activity was observed with both increased duration and frequency.</p>","PeriodicalId":13311,"journal":{"name":"Indian Journal of Dental Research","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Implant Osteoblast Differentiation Under Low-Magnitude High-Frequency Vibrational Loading - An In Vitro Study.\",\"authors\":\"Sachin Chaware, Chetan Kakade, Siddhesh Borse\",\"doi\":\"10.4103/ijdr.ijdr_125_24\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>The present study aimed to assess the microscopic effects of low-magnitude high frequency on dental implant surfaces using MG-63 cells and vertical vibration loading.</p><p><strong>Materials and methods: </strong>24 dental implants wrapped with collagen membranes were placed in a 12-well plate filled with MG-63 cell culture. A vibration device was constructed using 3D printing technology and employed a vertical vibration system. Three vibration cycles were utilized, including frequencies of 30 Hz, 60 Hz, and 90 Hz. Each cycle had durations of 1, 5, and 10 min/h, with a total duration of 6 h. The control group underwent no vibrational loading. After 24 h, analysis was conducted using an inverted microscope and a spectrophotometer. Alkaline phosphatase (ALP) expression was assessed using an ALP Kit.</p><p><strong>Results: </strong>The study yielded highly significant results (P < 0.001). cell activity was found to be highest with 30 Hz vibration loading compared to both 60 Hz and 90 Hz (P < 0.001). Among the various durations tested, the 30 Hz vibration with a 5-min cycle per hour, totalling 6 h, exhibited the highest cell activity. Additionally, ALP expression was most pronounced with 30 Hz vibrational loading. The cells displayed a dark green appearance surrounded by a pale green extracellular matrix.</p><p><strong>Conclusion: </strong>The highest cell activity and ALP expression were reported with the 30 Hz vibrational loading lasting 5 min/h, over a total duration of 6 h. However, a decline in cell activity was observed with both increased duration and frequency.</p>\",\"PeriodicalId\":13311,\"journal\":{\"name\":\"Indian Journal of Dental Research\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-06-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Indian Journal of Dental Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4103/ijdr.ijdr_125_24\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Dentistry\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Dental Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/ijdr.ijdr_125_24","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Dentistry","Score":null,"Total":0}
Implant Osteoblast Differentiation Under Low-Magnitude High-Frequency Vibrational Loading - An In Vitro Study.
Introduction: The present study aimed to assess the microscopic effects of low-magnitude high frequency on dental implant surfaces using MG-63 cells and vertical vibration loading.
Materials and methods: 24 dental implants wrapped with collagen membranes were placed in a 12-well plate filled with MG-63 cell culture. A vibration device was constructed using 3D printing technology and employed a vertical vibration system. Three vibration cycles were utilized, including frequencies of 30 Hz, 60 Hz, and 90 Hz. Each cycle had durations of 1, 5, and 10 min/h, with a total duration of 6 h. The control group underwent no vibrational loading. After 24 h, analysis was conducted using an inverted microscope and a spectrophotometer. Alkaline phosphatase (ALP) expression was assessed using an ALP Kit.
Results: The study yielded highly significant results (P < 0.001). cell activity was found to be highest with 30 Hz vibration loading compared to both 60 Hz and 90 Hz (P < 0.001). Among the various durations tested, the 30 Hz vibration with a 5-min cycle per hour, totalling 6 h, exhibited the highest cell activity. Additionally, ALP expression was most pronounced with 30 Hz vibrational loading. The cells displayed a dark green appearance surrounded by a pale green extracellular matrix.
Conclusion: The highest cell activity and ALP expression were reported with the 30 Hz vibrational loading lasting 5 min/h, over a total duration of 6 h. However, a decline in cell activity was observed with both increased duration and frequency.
期刊介绍:
Indian Journal of Dental Research (IJDR) is the official publication of the Indian Society for Dental Research (ISDR), India section of the International Association for Dental Research (IADR), published quarterly. IJDR publishes scientific papers on well designed and controlled original research involving orodental sciences. Papers may also include reports on unusual and interesting case presentations and invited review papers on significant topics.