低强度高频振动载荷下种植体成骨细胞分化的体外研究。

Q3 Dentistry
Sachin Chaware, Chetan Kakade, Siddhesh Borse
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引用次数: 0

摘要

摘要:本研究旨在利用MG-63细胞和垂直振动载荷,评估低量级高频对种植体表面的微观影响。材料和方法:将24颗牙种植体包裹胶原膜,置于充满MG-63细胞培养物的12孔板中。利用3D打印技术构建振动装置,采用垂直振动系统。使用了三个振动周期,包括30赫兹,60赫兹和90赫兹的频率。每个周期的持续时间分别为1、5和10分钟/小时,总持续时间为6小时。对照组不进行振动加载。24 h后用倒置显微镜和分光光度计进行分析。用ALP试剂盒检测碱性磷酸酶(ALP)的表达。结果:本研究取得了极显著的结果(P < 0.001)。与60 Hz和90 Hz振动载荷相比,30 Hz振动载荷下细胞活性最高(P < 0.001)。在不同的持续时间测试中,30 Hz振动,每小时5分钟,总共6小时,表现出最高的细胞活性。此外,在30 Hz的振动载荷下,ALP的表达最为明显。细胞呈深绿色,周围有淡绿色的细胞外基质。结论:30hz振动载荷持续5 min/h,总持续时间为6 h时,细胞活性和ALP表达最高。然而,随着持续时间和频率的增加,细胞活性下降。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Implant Osteoblast Differentiation Under Low-Magnitude High-Frequency Vibrational Loading - An In Vitro Study.

Introduction: The present study aimed to assess the microscopic effects of low-magnitude high frequency on dental implant surfaces using MG-63 cells and vertical vibration loading.

Materials and methods: 24 dental implants wrapped with collagen membranes were placed in a 12-well plate filled with MG-63 cell culture. A vibration device was constructed using 3D printing technology and employed a vertical vibration system. Three vibration cycles were utilized, including frequencies of 30 Hz, 60 Hz, and 90 Hz. Each cycle had durations of 1, 5, and 10 min/h, with a total duration of 6 h. The control group underwent no vibrational loading. After 24 h, analysis was conducted using an inverted microscope and a spectrophotometer. Alkaline phosphatase (ALP) expression was assessed using an ALP Kit.

Results: The study yielded highly significant results (P < 0.001). cell activity was found to be highest with 30 Hz vibration loading compared to both 60 Hz and 90 Hz (P < 0.001). Among the various durations tested, the 30 Hz vibration with a 5-min cycle per hour, totalling 6 h, exhibited the highest cell activity. Additionally, ALP expression was most pronounced with 30 Hz vibrational loading. The cells displayed a dark green appearance surrounded by a pale green extracellular matrix.

Conclusion: The highest cell activity and ALP expression were reported with the 30 Hz vibrational loading lasting 5 min/h, over a total duration of 6 h. However, a decline in cell activity was observed with both increased duration and frequency.

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来源期刊
Indian Journal of Dental Research
Indian Journal of Dental Research Dentistry-Dentistry (all)
CiteScore
1.80
自引率
0.00%
发文量
80
审稿时长
38 weeks
期刊介绍: Indian Journal of Dental Research (IJDR) is the official publication of the Indian Society for Dental Research (ISDR), India section of the International Association for Dental Research (IADR), published quarterly. IJDR publishes scientific papers on well designed and controlled original research involving orodental sciences. Papers may also include reports on unusual and interesting case presentations and invited review papers on significant topics.
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