Tianyi Feng, Liping Wei, Changhui Zhou, Shenning Li, Yingqi Li, Yali Fang, Wenteng Cui, Xiaohui Li, Lingzhi Bao, Lei Shen, Min Tang, Yan Chang
{"title":"基于光的生物3D打印睾丸类器官作为生殖毒性评估的体外模型。","authors":"Tianyi Feng, Liping Wei, Changhui Zhou, Shenning Li, Yingqi Li, Yali Fang, Wenteng Cui, Xiaohui Li, Lingzhi Bao, Lei Shen, Min Tang, Yan Chang","doi":"10.1021/acsbiomaterials.5c00414","DOIUrl":null,"url":null,"abstract":"<p><p>Organoids can be used as an in vitro platform for studying tissue development and toxicology evaluation. While in vitro maturation of somatic and germ cells has been demonstrated in organoids, generating reproducible primary testicular cell-derived organoids for toxicity evaluation remains challenging. In this study, we developed testicular organoids using light-based 3D bioprinting of neonatal mouse primary testicular cells on transwell inserts. The bioprinting ink composition was specifically designed and optimized based on the extracellular matrix composition and mechanical properties of testicular tissue. The organoids were cultured for 21 days using an optimized medium to support testicular cell development and function. These bioprinted organoids recapitulated key features of the in vivo testicular architecture, forming tubule-like structures with cellular organization and gene expression profiles similar to native tissue. Following exposure to the known male reproductive toxic agent triptolide, testicular organoids showed loss of tight junction protein CLAUDIN-11 and altered transcript levels of somatic markers. Each bioprinted testicular organoid can be generated within 1 min, with toxicity evaluation results available within 1 month. This rapid turnaround makes it a promising high-throughput platform for toxicological studies, advancing our understanding of testicular development, function, and the impact of potential toxicants on male reproductive health.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4000,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Light-Based 3D Bioprinting of Testicular Organoid as an In Vitro Model for Reproductive Toxicity Assessment.\",\"authors\":\"Tianyi Feng, Liping Wei, Changhui Zhou, Shenning Li, Yingqi Li, Yali Fang, Wenteng Cui, Xiaohui Li, Lingzhi Bao, Lei Shen, Min Tang, Yan Chang\",\"doi\":\"10.1021/acsbiomaterials.5c00414\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Organoids can be used as an in vitro platform for studying tissue development and toxicology evaluation. While in vitro maturation of somatic and germ cells has been demonstrated in organoids, generating reproducible primary testicular cell-derived organoids for toxicity evaluation remains challenging. In this study, we developed testicular organoids using light-based 3D bioprinting of neonatal mouse primary testicular cells on transwell inserts. The bioprinting ink composition was specifically designed and optimized based on the extracellular matrix composition and mechanical properties of testicular tissue. The organoids were cultured for 21 days using an optimized medium to support testicular cell development and function. These bioprinted organoids recapitulated key features of the in vivo testicular architecture, forming tubule-like structures with cellular organization and gene expression profiles similar to native tissue. Following exposure to the known male reproductive toxic agent triptolide, testicular organoids showed loss of tight junction protein CLAUDIN-11 and altered transcript levels of somatic markers. Each bioprinted testicular organoid can be generated within 1 min, with toxicity evaluation results available within 1 month. This rapid turnaround makes it a promising high-throughput platform for toxicological studies, advancing our understanding of testicular development, function, and the impact of potential toxicants on male reproductive health.</p>\",\"PeriodicalId\":8,\"journal\":{\"name\":\"ACS Biomaterials Science & Engineering\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":5.4000,\"publicationDate\":\"2025-06-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Biomaterials Science & Engineering\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1021/acsbiomaterials.5c00414\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Biomaterials Science & Engineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1021/acsbiomaterials.5c00414","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
Light-Based 3D Bioprinting of Testicular Organoid as an In Vitro Model for Reproductive Toxicity Assessment.
Organoids can be used as an in vitro platform for studying tissue development and toxicology evaluation. While in vitro maturation of somatic and germ cells has been demonstrated in organoids, generating reproducible primary testicular cell-derived organoids for toxicity evaluation remains challenging. In this study, we developed testicular organoids using light-based 3D bioprinting of neonatal mouse primary testicular cells on transwell inserts. The bioprinting ink composition was specifically designed and optimized based on the extracellular matrix composition and mechanical properties of testicular tissue. The organoids were cultured for 21 days using an optimized medium to support testicular cell development and function. These bioprinted organoids recapitulated key features of the in vivo testicular architecture, forming tubule-like structures with cellular organization and gene expression profiles similar to native tissue. Following exposure to the known male reproductive toxic agent triptolide, testicular organoids showed loss of tight junction protein CLAUDIN-11 and altered transcript levels of somatic markers. Each bioprinted testicular organoid can be generated within 1 min, with toxicity evaluation results available within 1 month. This rapid turnaround makes it a promising high-throughput platform for toxicological studies, advancing our understanding of testicular development, function, and the impact of potential toxicants on male reproductive health.
期刊介绍:
ACS Biomaterials Science & Engineering is the leading journal in the field of biomaterials, serving as an international forum for publishing cutting-edge research and innovative ideas on a broad range of topics:
Applications and Health – implantable tissues and devices, prosthesis, health risks, toxicology
Bio-interactions and Bio-compatibility – material-biology interactions, chemical/morphological/structural communication, mechanobiology, signaling and biological responses, immuno-engineering, calcification, coatings, corrosion and degradation of biomaterials and devices, biophysical regulation of cell functions
Characterization, Synthesis, and Modification – new biomaterials, bioinspired and biomimetic approaches to biomaterials, exploiting structural hierarchy and architectural control, combinatorial strategies for biomaterials discovery, genetic biomaterials design, synthetic biology, new composite systems, bionics, polymer synthesis
Controlled Release and Delivery Systems – biomaterial-based drug and gene delivery, bio-responsive delivery of regulatory molecules, pharmaceutical engineering
Healthcare Advances – clinical translation, regulatory issues, patient safety, emerging trends
Imaging and Diagnostics – imaging agents and probes, theranostics, biosensors, monitoring
Manufacturing and Technology – 3D printing, inks, organ-on-a-chip, bioreactor/perfusion systems, microdevices, BioMEMS, optics and electronics interfaces with biomaterials, systems integration
Modeling and Informatics Tools – scaling methods to guide biomaterial design, predictive algorithms for structure-function, biomechanics, integrating bioinformatics with biomaterials discovery, metabolomics in the context of biomaterials
Tissue Engineering and Regenerative Medicine – basic and applied studies, cell therapies, scaffolds, vascularization, bioartificial organs, transplantation and functionality, cellular agriculture