脂质双分子层与膜蛋白之间的变构偶联

IF 3.2 3区 生物学 Q2 BIOPHYSICS
Clarisse Fourel, Yanna Gautier, Alexandre Pozza, François Giraud, Elodie Point, Christel Le Bon, Karine Moncoq, Guillaume Stirnemann, Jérôme Hénin, Ewen Lescop, Laurent J. Catoire
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引用次数: 0

摘要

生物膜是一个复杂的环境,其功能与脂质和蛋白质之间的动态相互作用密切相关。在这里,我们利用脂质纳米盘的高压核磁共振与分子动力学模拟相结合,在原子尺度上阐明了脂质双分子层与模型膜蛋白OmpX之间的变构对话。我们发现OmpX通过在蛋白质表面的疏水和粗糙度匹配过程液化脂质的环状壳,从而延迟凝胶化过程。此外,脂质双分子层力学性质的改变直接影响了脂质/蛋白质界面上氨基酸侧链的能量格局,而且出乎意料地影响了蛋白质核心。我们的工作强调了一个热力学耦合但动力学不耦合的变构途径,将脂质动力学与膜蛋白内部联系起来,直接影响我们对膜功能的理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Allosteric coupling between a lipid bilayer and a membrane protein
Biological membranes are complex environments whose functions are closely tied to the dynamic interactions between lipids and proteins. Here, we utilize high-pressure NMR of lipid nanodiscs paired with molecular dynamics simulations to elucidate at the atomic scale the allosteric dialog between the lipid bilayer and a model membrane protein, OmpX. We discover that OmpX delays the gelation process by liquefying the annular shell of lipids through hydrophobic and roughness matching processes at the protein surface. Furthermore, modification of the mechanical properties of the lipid bilayer directly impacts the energy landscape of amino acid side chains at the lipid/protein interface but also unexpectedly at the protein core. Our work highlights a thermodynamically coupled but kinetically uncoupled allosteric pathway linking lipid dynamics with the interior of membrane proteins, directly impacting our understanding of membrane function.
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来源期刊
Biophysical journal
Biophysical journal 生物-生物物理
CiteScore
6.10
自引率
5.90%
发文量
3090
审稿时长
2 months
期刊介绍: BJ publishes original articles, letters, and perspectives on important problems in modern biophysics. The papers should be written so as to be of interest to a broad community of biophysicists. BJ welcomes experimental studies that employ quantitative physical approaches for the study of biological systems, including or spanning scales from molecule to whole organism. Experimental studies of a purely descriptive or phenomenological nature, with no theoretical or mechanistic underpinning, are not appropriate for publication in BJ. Theoretical studies should offer new insights into the understanding ofexperimental results or suggest new experimentally testable hypotheses. Articles reporting significant methodological or technological advances, which have potential to open new areas of biophysical investigation, are also suitable for publication in BJ. Papers describing improvements in accuracy or speed of existing methods or extra detail within methods described previously are not suitable for BJ.
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