胡椒精油和苯丙素：对玉米象和木薯的生物活性和酶抑制作用

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research Pub Date : 2025-06-28 DOI:10.1016/j.jspr.2025.102714

Daniel Camilo Ripoll-Aristizábal , Oscar Javier Patiño-Ladino , Juliet Angélica Prieto-Rodríguez

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引用次数: 0

摘要

本研究旨在评价四种胡椒精油和选定的苯丙类杀虫剂对玉米象和玉米三角虫的杀虫潜力，重点研究熏蒸、局部毒性、驱避作用以及与解毒和运动功能相关的酶抑制作用。采用水蒸气蒸馏法提取了沙刺柏（Piper aduncum）、沙刺柏（P. asperiusculum）、金穗柏（P. aurum）和黑刺柏（P. holtonii）的EOs，并用GC-MS进行了分析。用闪蒸色谱法从精油中分离出皂角醇、皂角醇和肉豆蔻素，而黄樟油则通过商业途径获得。熏蒸剂的活性采用小瓶法测试，局部毒性通过直接应用，驱避通过区域偏好法测试。采用分光光度法测定乙酰胆碱酯酶（AChE）、过氧化氢酶（CAT）和谷胱甘肽s -转移酶（GST）。其主要成分为：灰荆荆中含有皂素和皂素，荆荆中含有肉豆蔻素和皂素，金荆中含有红花素，黑荆中含有皂素和皂素。对蓖麻有较强的熏蒸和局部毒力(LC50: 4.7 ~ 7.2 μL/L；LD50: 84.4 ~ 120.0 μg/虫)，而草皮草EO对玉米玉米瘟病菌的局部杀伤有效（LD50: 108.2 ~ 123.2 μg/虫）。在0.1 μL/cm2的驱避率分别为90%和80%。黄樟酚对玉米螟和甘蔗螟的LD50分别为34.4和12.6 μg/虫，毒性最强。构效分析表明，甲氧基越多，毒性越低。酶抑制范围从低到中度（0 - 50%），表明二次参与杀虫作用。这些结果支持Piper EOs和苯丙素作为生态友好型害虫防治剂的潜力。

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Essential oils and phenylpropanoids from Piper: Bioactivity and enzyme inhibition in Sitophilus zeamais and Tribolium castaneum

This study aimed to evaluate the insecticidal potential of essential oils (EOs) from four Piper species and selected phenylpropanoids against Triboliu

m castaneum and Sitophilus zeamais, focusing on fumigant, topical toxicity, repellent effects, and enzyme inhibition related to detoxification and motor function. EOs from Piper aduncum, P. asperiusculum, P. auritum, and P. holtonii were obtained by steam distillation and analyzed by GC-MS. Apiol, dillapiole, and myristicin were isolated from the EOs using flash chromatography, while safrole was acquired commercially. Fumigant activity was tested using the vial-in-vial method, topical toxicity via direct application, and repellency through the area preference method. Enzymatic assays for acetylcholinesterase (AChE), catalase (CAT), and glutathione S-transferase (GST) were performed spectrophotometrically. Major constituents included dillapiole and apiol in P. aduncum, myristicin and dillapiole in P. asperiusculum, safrole in P. auritum, and apiol and dillapiole in P. holtonii. P. auritum EO showed potent fumigant and topical toxicity against T. castaneum (LC₅₀: 4.7–7.2 μL/L air; LD₅₀: 84.4–120.0 μg/insect), while P. asperiusculum EO was effective topically against S. zeamais (LD₅₀: 108.2–123.2 μg/insect). P. holtonii EO was highly repellent (90 % and 80 % repellency at 0.1 μL/cm²). Safrole exhibited the highest toxicity among constituents (LD₅₀: 34.4 and 12.6 μg/insect for S. zeamais and T. castaneum, respectively). Structure–activity analysis indicated decreased toxicity with more methoxy groups. Enzyme inhibition ranged from low to moderate (0–50 %), suggesting secondary involvement in insecticidal action. These results support the potential of Piper EOs and phenylpropanoids as eco-friendly pest control agents.

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来源期刊

Journal of Stored Products Research 生物-昆虫学

CiteScore

5.70

自引率

18.50%

发文量

112

审稿时长

45 days

期刊介绍： The Journal of Stored Products Research provides an international medium for the publication of both reviews and original results from laboratory and field studies on the preservation and safety of stored products, notably food stocks, covering storage-related problems from the producer through the supply chain to the consumer. Stored products are characterised by having relatively low moisture content and include raw and semi-processed foods, animal feedstuffs, and a range of other durable items, including materials such as clothing or museum artefacts.