杂交对两种杂交石斑鱼线粒体DNA的影响

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-06-19 DOI:10.1016/j.genrep.2025.102279

Liu Cao , Jun Ma , Binbin Li , Hanwen Fu , Yan Lu , Yatong Wu , Pan Chen , Xingrong Hou , Ning Yang , Hai Huang

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引用次数: 0

摘要

动物线粒体DNA （mtDNA）遵循严格的母系遗传规律。杂交事件可能对杂种mtDNA的遗传模式产生影响。为了研究杂交对不同杂交石斑鱼mtDNA的影响，采用新一代测序技术测定了cromilepetes altivelis × Epinephelus lanceolatus（Hyb1）、E. fuscoguttatus × E. polyhekadion （Hyb2）及其亲本的有丝分裂基因组。基于细胞色素c氧化酶3 （cox3）部分序列、trnG序列和NADH脱氢酶亚基3 （nad3）序列，比较分析了两种杂交石斑鱼的遗传多样性和单倍型网络。结果表明，两种杂交石斑鱼的mtDNA均为母系遗传，序列相似性达99.8%。它们的有丝分裂基因组具有相似的基因结构。然而，在两种杂交石斑鱼中发现了不同程度的核苷酸变异。在这两个杂交种中，Hyb1的遗传多样性高于Hyb2。我们的观察结果为理解杂交对杂种mtDNA的影响提供了重要的知识。

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Impact of hybridization on mitochondrial DNA in two kinds of hybrid groupers

Animal mitochondrial DNA (mtDNA) follows the general rule of strict maternal inheritance. The event of hybridization could have an impact on the inherited pattern of mtDNA in hybrid. To investigate the impact of hybridization on mtDNA in different hybrid groupers, the mitogenomes of Cromileptes altivelis × Epinephelus lanceolatus(Hyb1), E. fuscoguttatus × E. polyphekadion (Hyb2), and their parents were determined using next-generation sequencing. Genetic diversity and haplotype network were also comparatively analyzed among two hybrid groupers based on the partial cytochrome c oxidase 3 (cox3), trnG and NADH dehydrogenase subunit 3 (nad3) sequences. Our results confirmed that mtDNA of two hybrid groupers were maternal inheritance, showing 99.8 % sequence similarity with their respective female parent. Their mitogenomes presented similar gene architectures. However, the different degrees of nucleotide variation have been detected in two hybrid groupers. Among these two hybrids, Hyb1 presented a higher level of genetic diversity compared with Hyb2. Our observations provide a significant knowledge to understand the effect of hybridization on mtDNA in hybrids.

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.