南非蛔虫β-微管蛋白同型1基因与苯并咪唑抗性相关的单核苷酸多态性

IF 3 4区医学 Q2 INFECTIOUS DISEASES

Brazilian Journal of Infectious Diseases Pub Date : 2025-06-28 DOI:10.1016/j.bjid.2025.104556

Teniel Ramkhelawan , Pragalathan Naidoo , Zilungile L. Mkhize-Kwitshana

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引用次数: 0

摘要

蛔虫病是一种由类蛔虫引起的寄生虫感染，全世界有超过12亿人感染。苯并咪唑（BZ）药物仍然是全球大规模驱虫计划的标准治疗方法。由于BZ类药物的广泛应用，蚓类β-微管蛋白基因（F200Y、E198A和F167Y）单核苷酸多态性（snp）的发生率越来越高。目的调查上述snp在南非成年人群中的流行情况。方法：这是主要横断面研究的一项亚研究，参与者（n = 414）从南非夸祖鲁-纳塔尔省德班南部城郊地区的五个公共卫生诊所招募。在目前的研究中，有目的地选择了20个粪便样本，这些粪便样本对蚓状拟虫卵呈阳性。阳性对照为4只拟南麻虫提取物。使用Sanger测序和RFLP-PCR来鉴定突变的存在。结果未发现突变，密码子F167Y、E198A和F200Y基因型均为纯合野生型。结论虽然在这个小的研究中没有发现突变，但不能排除在更大的样本子集中发生突变的可能性。

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Single nucleotide polymorphisms associated with benzimidazole resistance of the β-tubulin isotype 1 gene in Ascaris lumbricoides isolated in South Africa

Background

Ascariasis is a parasitic infection caused by Ascaris lumbricoides and infects over 1.2 billion people worldwide. Benzimidazole (BZ) drugs remain the standard treatment in large-scale deworming programs globally. The prevalence of Single Nucleotide Polymorphisms (SNPs) in the β-tubulin gene of A. lumbricoides (F200Y, E198A and F167Y) is increasing due to the widespread use of BZ drugs.

Aim

To investigate the prevalence of the above-mentioned SNPs in a South African adult population.

Methods

This was a sub-study of the main cross-sectional study with participants (n = 414) who had been recruited from five public health clinics in the peri‑urban areas South of Durban, KwaZulu-Natal, South Africa. For the current study, a purposive selection of 20 stool samples that were positive for A. lumbricoides eggs was made. A. lumbricoides worm extracts (n = 4) were used as a positive control. Sanger sequencing and RFLP-PCR were used to identify the presence of mutations.

Results

No mutations were detected, and all genotypes observed at codons F167Y, E198A and F200Y were the homozygous wild-type genotype.

Conclusion

Although no mutations were found in this small study, the potential occurrence of mutations in a larger sample subset cannot be ruled out.

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来源期刊

Brazilian Journal of Infectious Diseases 医学-传染病学

CiteScore

5.50

自引率

0.00%

发文量

925

审稿时长

41 days

期刊介绍： The Brazilian Journal of Infectious Diseases is the official publication of the Brazilian Society of Infectious Diseases (SBI). It aims to publish relevant articles in the broadest sense on all aspects of microbiology, infectious diseases and immune response to infectious agents. The BJID is a bimonthly publication and one of the most influential journals in its field in Brazil and Latin America with a high impact factor, since its inception it has garnered a growing share of the publishing market.