移植胰岛到预血管化的皮下部位以提高移植存活率和功能：一个小鼠模型。

IF 0.8 4区医学 Q4 IMMUNOLOGY

Transplantation proceedings Pub Date : 2025-07-01 DOI:10.1016/j.transproceed.2025.05.019

Tsuyoshi Okada , Takashi Kuise , Kenjiro Kumano , Hiroshi Tazawa , Toshiyoshi Fujiwara

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引用次数: 0

摘要

背景：皮下间隙被认为是胰岛移植的潜在部位；然而，低氧条件是一个主要的缺点。我们假设使用体内组织构建技术将胰岛移植到预血管化的皮下空间将改善胰岛的植入并有助于维持移植胰岛的功能。方法：采用硅胶棒植入C57BL/6J型糖尿病小鼠血管前化皮下间隙（PSS），将500个同种胰岛移植2周。将胰岛移植到未经修饰的皮下间隙（USS）的糖尿病小鼠作为对照。移植后监测非空腹血糖水平8周，并进行腹腔内糖耐量试验和组织学评价。结果：PSS组10例患者中有5例血糖恢复正常，USS组9例患者中有2例血糖恢复正常。PSS组10例受者中有9例移植胰岛移植，PSS组移植胰岛移植率显著高于USS组（P = 0.005）。腹腔内糖耐量试验结果显示，PSS组受体小鼠的糖耐量明显优于糖尿病小鼠。组织学分析显示，PSS组移植胰岛周围结缔组织毛细血管密度显著高于USS组（41.7/mm2 vs 7.2/mm2, P < 0.01）。结论：采用体内组织构建技术将胰岛移植到PSS内具有提高移植物存活率的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Grafting Islets to a Prevascularized Subcutaneous Site to Improve Transplant Survival and Function: A Mouse Model

Background

The subcutaneous space is regarded as a potential site for islet transplantation; however, hypoxic conditions are a major drawback. We hypothesized that islet transplantation into a prevascularized subcutaneous space created using in-body tissue architecture technology would improve islet engraftment and contribute to maintaining the function of transplanted islets.

Methods

Five hundred syngeneic islets were transplanted into the prevascularized subcutaneous space (PSS) constructed by the implantation of a silicone rod for 2 weeks in C57BL/6J diabetic mice. Diabetic mice transplanted with islets into the unmodified subcutaneous space (USS) were used as the controls. After transplantation, nonfasting blood glucose levels were monitored for 8 weeks, and an intraperitoneal glucose tolerance test and histological evaluation were performed.

Results

Five of the 10 recipients in the PSS group and two of the nine recipients in the USS group returned to normoglycemia. Engraftment of the transplanted islets was detected in nine of the 10 recipients in the PSS group, and the engraftment rate in the PSS group was significantly higher than that in the USS group (P = .005). Intraperitoneal glucose tolerance test results showed that the glucose tolerance of recipient mice in the PSS group was significantly better than that of the diabetic mice. Histological analysis revealed that the density of capillary vessels in the connective tissue surrounding the engrafted islets in the PSS group was significantly higher than that in the USS group (41.7/mm² vs 7.2/mm², P < .01).

Conclusion

Islet transplantation into the PSS using in-body tissue architecture technology has the potential to improve graft survival.

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来源期刊

Transplantation proceedings 医学-免疫学

CiteScore

1.70

自引率

0.00%

发文量

502

审稿时长

60 days

期刊介绍： Transplantation Proceedings publishes several different categories of manuscripts, all of which undergo extensive peer review by recognized authorities in the field prior to their acceptance for publication. The first type of manuscripts consists of sets of papers providing an in-depth expression of the current state of the art in various rapidly developing components of world transplantation biology and medicine. These manuscripts emanate from congresses of the affiliated transplantation societies, from Symposia sponsored by the Societies, as well as special Conferences and Workshops covering related topics. Transplantation Proceedings also publishes several special sections including publication of Clinical Transplantation Proceedings, being rapid original contributions of preclinical and clinical experiences. These manuscripts undergo review by members of the Editorial Board. Original basic or clinical science articles, clinical trials and case studies can be submitted to the journal?s open access companion title Transplantation Reports.