{"title":"野生型和杂合型斑球蟒的PCR和qPCR鉴别。","authors":"R Kumsiri, N N Yin, P Kanchanaphum","doi":"10.1590/1519-6984.293718","DOIUrl":null,"url":null,"abstract":"<p><p>The ball python, also known as Python regius, is a popular exotic pet due to its beautiful colors and patterns, particularly the piebald variety. However, it can be challenging to distinguish between the wild type and heterozygous piebald (het piebald) phenotypes. This study employs PCR and qPCR techniques to differentiate between these two genotypes. The PCR product size for both the wild type and het piebald was 255 bp. However, the intensity of the PCR product from the wild type was twice that of the het piebald, and no PCR product was detected in the piebald individuals. Additionally, the Ct value for the wild type was lower than that of the het piebald. The qPCR analysis revealed a 15.45% difference between the two groups. Therefore, PCR and qPCR effectively distinguish between the wild type and het piebald genotypes of ball pythons.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e293718"},"PeriodicalIF":0.0000,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Discrimination between wild type and heterozygous piebald ball python (Python regius) by PCR and qPCR.\",\"authors\":\"R Kumsiri, N N Yin, P Kanchanaphum\",\"doi\":\"10.1590/1519-6984.293718\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The ball python, also known as Python regius, is a popular exotic pet due to its beautiful colors and patterns, particularly the piebald variety. However, it can be challenging to distinguish between the wild type and heterozygous piebald (het piebald) phenotypes. This study employs PCR and qPCR techniques to differentiate between these two genotypes. The PCR product size for both the wild type and het piebald was 255 bp. However, the intensity of the PCR product from the wild type was twice that of the het piebald, and no PCR product was detected in the piebald individuals. Additionally, the Ct value for the wild type was lower than that of the het piebald. The qPCR analysis revealed a 15.45% difference between the two groups. Therefore, PCR and qPCR effectively distinguish between the wild type and het piebald genotypes of ball pythons.</p>\",\"PeriodicalId\":55326,\"journal\":{\"name\":\"Brazilian Journal of Biology\",\"volume\":\"85 \",\"pages\":\"e293718\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-06-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Brazilian Journal of Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1590/1519-6984.293718\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brazilian Journal of Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1590/1519-6984.293718","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Discrimination between wild type and heterozygous piebald ball python (Python regius) by PCR and qPCR.
The ball python, also known as Python regius, is a popular exotic pet due to its beautiful colors and patterns, particularly the piebald variety. However, it can be challenging to distinguish between the wild type and heterozygous piebald (het piebald) phenotypes. This study employs PCR and qPCR techniques to differentiate between these two genotypes. The PCR product size for both the wild type and het piebald was 255 bp. However, the intensity of the PCR product from the wild type was twice that of the het piebald, and no PCR product was detected in the piebald individuals. Additionally, the Ct value for the wild type was lower than that of the het piebald. The qPCR analysis revealed a 15.45% difference between the two groups. Therefore, PCR and qPCR effectively distinguish between the wild type and het piebald genotypes of ball pythons.
期刊介绍：
The BJB – Brazilian Journal of Biology® is a scientific journal devoted to publishing original articles in all fields of the Biological Sciences, i.e., General Biology, Cell Biology, Evolution, Biological Oceanography, Taxonomy, Geographic Distribution, Limnology, Aquatic Biology, Botany, Zoology, Genetics, and Ecology. Priority is given to papers presenting results of researches in the Neotropical region. Material published includes research papers, review papers (upon approval of the Editorial Board), notes, book reviews, and comments.
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