利用DESS和DNA提取技术保存博物馆藏品中的DNA标本。

IF 3.6 3区 生物学 Q1 BIOLOGY
Eri Ogiso-Tanaka, Daisuke Shimada, Akito Ogawa, Genki Ishiyama, Ken-Ichi Okumura, Kentaro Hosaka, Chikako Ishii, Kyung-Ok Nam, Masakazu Hoshino, Shuhei Nomura, Showtaro Kakizoe, Yasuhide Nakamura, Isao Nishiumi, Minako Abe Ito, Taiju Kitayama, Norio Tanaka, Tsuyoshi Hosoya, Utsugi Jinbo
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引用次数: 0

摘要

DNA研究的最新进展增加了博物馆不仅要保存形态学标本,还要保存它们的DNA的必要性,这导致我们在-80°C下保存与标本相关的组织样本。DNA分析已成为生物分类学研究和生物多样性评价的重要工具;然而,由于空间限制和操作成本,所有样品的冷冻储存是不切实际的。这就迫切需要开发更广泛适用的DNA保存方法。研究了传统保存方法与DESS (DMSO/EDTA/饱和NaCl溶液)保存方法在形态学和DNA完整性方面的比较效果。我们的研究结果表明,在室温下,DESS保存在所有被检测物种中都能保持高质量的DNA片段,甚至在10年的储存后,线虫样本也能保持DNA的完整性。当保存整个生物体时,保持形态特征和DNA完整性的最佳保存溶液条件因物种而异。值得注意的是,即使在DESS溶液完全蒸发后,DNA的完整性仍保持不变。这些发现表明,DESS用于标本DNA保存在许多物种中都是有效的,不仅可以在没有冷冻设施的环境中长期保存,还可以在冷冻前临时保存。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
DNA Specimen Preservation Using DESS and DNA Extraction in Museum Collections.

Recent advances in DNA research have increased the necessity for museums to preserve not only morphological specimens but also their DNA, leading us to maintain tissue samples linked to specimens at -80 °C. DNA analysis has become an essential tool for taxonomic research and biodiversity assessment; however, freezer storage for all samples is impractical due to space limitations and operational costs. This creates a pressing need to develop more widely applicable DNA preservation methods. We investigated the comparative effects of traditional preservation methods versus DESS (DMSO/EDTA/saturated NaCl solution) preservation on both morphology and DNA integrity using museum specimens from various taxonomic groups. Our results demonstrated that DESS preservation maintained high-quality DNA fragments exceeding >15 kb at room temperature across all examined species, with nematode samples maintaining DNA integrity even after 10 years of storage. When preserving whole organisms, the optimal preservation solution conditions for maintaining both morphological features and DNA integrity varied among species. Notably, DNA integrity was maintained even after complete evaporation of the DESS solution. These findings suggest that DESS utilization for specimen DNA preservation is effective across many species, not only for long-term storage in environments without freezer facilities but also for temporary preservation until freezing.

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来源期刊
Biology-Basel
Biology-Basel Biological Science-Biological Science
CiteScore
5.70
自引率
4.80%
发文量
1618
审稿时长
11 weeks
期刊介绍: Biology (ISSN 2079-7737) is an international, peer-reviewed, quick-refereeing open access journal of Biological Science published by MDPI online. It publishes reviews, research papers and communications in all areas of biology and at the interface of related disciplines. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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