M C F Cheng, M R V Welford, L N Zamora, N J Delorme, N L C Ragg, A J R Hickey, B J Dunphy
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Addressing these issues requires identifying treatments to reduce post-harvest stress and understanding underpinning molecular mechanisms.</p><p><strong>Objective: </strong>This study aimed to evaluate treatments (low temperature and MgCl<sub>2</sub> anaesthetic baths) to mitigate post-harvest handling stress in mussels.</p><p><strong>Methods: </strong>We analysed metabolomic profiles using gas chromatography-mass spectrometry (GC/MS), anaerobic enzyme activity in gill and adductor muscle, and haemolymph biochemistry (pH, antioxidant capacity and osmolality) in mussels subjected to 14 °C, 4 °C or MgCl<sub>2</sub> water-bath treatments after simulated harvest.</p><p><strong>Results: </strong>Metabolomic analyses revealed post-harvest mussels experienced increased anaerobic activity, osmotic and oxidative stress, reduced pH (Δ0.31), and lower polyunsaturated fatty acids (PUFA). Mussels immersed in 14 °C seawater recovered from anaerobiosis but had a strong indication of oxidative stress. Although mussels in 4 °C immersion had increased levels of PUFA, implying depressed lipid oxidation, the treatment did not improve recovery from anaerobiosis, indicated by reduced pH (Δ0.38). Mussels treated with MgCl<sub>2</sub> showed some recovery from anaerobic handling stress, with decreased anaerobic end product accumulation and a more modest haemolymph pH decline (Δ0.16) compared to controls. 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引用次数: 0
摘要
通过加强采后处理，可以进一步提高出口青唇贻贝（Perna canaliculus）的存活率和品质，旨在减少与运输出水有关的生理应激。解决这些问题需要确定减少收获后应激的处理方法，并了解基本的分子机制。目的：本研究旨在评估低温和MgCl2麻醉浴对贻贝收获后处理应激的影响。方法：我们使用气相色谱-质谱（GC/MS）分析了模拟收获后经过14°C、4°C或MgCl2水浴处理的贻贝的代谢组学特征、鳃和内收肌的厌氧酶活性以及血淋巴生化（pH、抗氧化能力和渗透压）。结果：代谢组学分析显示，收获后贻贝的厌氧活性、渗透和氧化应激增加，pH值降低（Δ0.31），多不饱和脂肪酸（PUFA）降低。浸泡在14°C海水中的贻贝从厌氧症中恢复过来，但有强烈的氧化应激迹象。虽然浸泡在4°C中的贻贝PUFA水平增加，这意味着脂质氧化受到抑制，但处理并没有改善厌氧症的恢复，这表明pH值降低（Δ0.38）。与对照组相比，MgCl2处理的贻贝从厌氧处理应激中有所恢复，厌氧终产物积累减少，血淋巴pH值下降更温和（Δ0.16）。虽然厌氧酶活性表现出组织特异性反应，但它们并没有表现出其产物在代谢谱中所显示的显著差异。结论：在建议的再浸泡处理中，将贻贝浸泡在含有40 g L- 1 MgCl2的海水中似乎是缓解收获后代谢应激的最有效处理，因此可能会延长贻贝的货架期。
Metabolomic and physiological insights to ameliorate post-harvest stress in cultured mussels.
Introduction: Survival and quality of Green-lipped mussels (Perna canaliculus) exported live could be further improved through enhanced post-harvest handling, aiming to reduce the physiological stress associated with transport out of water. Addressing these issues requires identifying treatments to reduce post-harvest stress and understanding underpinning molecular mechanisms.
Objective: This study aimed to evaluate treatments (low temperature and MgCl2 anaesthetic baths) to mitigate post-harvest handling stress in mussels.
Methods: We analysed metabolomic profiles using gas chromatography-mass spectrometry (GC/MS), anaerobic enzyme activity in gill and adductor muscle, and haemolymph biochemistry (pH, antioxidant capacity and osmolality) in mussels subjected to 14 °C, 4 °C or MgCl2 water-bath treatments after simulated harvest.
Results: Metabolomic analyses revealed post-harvest mussels experienced increased anaerobic activity, osmotic and oxidative stress, reduced pH (Δ0.31), and lower polyunsaturated fatty acids (PUFA). Mussels immersed in 14 °C seawater recovered from anaerobiosis but had a strong indication of oxidative stress. Although mussels in 4 °C immersion had increased levels of PUFA, implying depressed lipid oxidation, the treatment did not improve recovery from anaerobiosis, indicated by reduced pH (Δ0.38). Mussels treated with MgCl2 showed some recovery from anaerobic handling stress, with decreased anaerobic end product accumulation and a more modest haemolymph pH decline (Δ0.16) compared to controls. While anaerobic enzyme activities showed tissue-specific responses, they did not exhibit the pronounced differences among treatments shown by their products in metabolic profiling.
Conclusion: Among the proposed re-immersion treatments, immersing mussels in seawater containing 40 g L- 1 MgCl2 seemed to be the most effective treatment to alleviate post-harvest metabolic stress, therefore potentially increasing shelf-life of mussels destined for live export.
期刊介绍：
Metabolomics publishes current research regarding the development of technology platforms for metabolomics. This includes, but is not limited to:
metabolomic applications within man, including pre-clinical and clinical
pharmacometabolomics for precision medicine
metabolic profiling and fingerprinting
metabolite target analysis
metabolomic applications within animals, plants and microbes
transcriptomics and proteomics in systems biology
Metabolomics is an indispensable platform for researchers using new post-genomics approaches, to discover networks and interactions between metabolites, pharmaceuticals, SNPs, proteins and more. Its articles go beyond the genome and metabolome, by including original clinical study material together with big data from new emerging technologies.
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