{"title":"泰国大麻条、糖叶提取物对病原菌的体外抑菌活性研究。","authors":"Panicha Pongnaratorn, Natthida Sophon, Parichart Boueroy","doi":"10.5455/javar.2025.l870","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong><i>Cannabis sativa</i> L. is aware of a rich source of bioactive substances with various structures that exhibit pharmacological activity in the central nervous system, cardiovascular, cerebrovascular, respiratory, reproductive, and gastrointestinal systems.</p><p><strong>Materials and methods: </strong>In this study, cannabis sugar leaves were soaked in 99% ethanol, followed by evaporation. The antibacterial effect of the cannabis sugar leaf extract was then evaluated using the disc diffusion method. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined using broth dilution.</p><p><strong>Results: </strong>The results of this study indicated that the cannabis sugar leaf extract inhibited <i>Bacillus cereus</i>, <i>Vibrio cholerae</i>, <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, and <i>Staphylococcus epidermidis</i> when compared to tetracycline, but it did not inhibit <i>Pseudomonas</i> <i>aeruginosa</i>. The MIC and MBC of the cannabis sugar leaves extract against <i>B</i>. <i>cereus</i>, <i>V</i>. <i>cholerae</i>, <i>E</i>. <i>coli</i>, <i>S</i>. <i>aureus</i>, and <i>S</i>. <i>epidermidis</i> were 0.977, 1.953, 31.25, 62.5, 125, 250, 250, 500, 250, and 500 mg/ml, respectively. The bioactive compounds in cannabis sugar leaf extract were identified using high-performance liquid chromatography.</p><p><strong>Conclusion: </strong>The results indicated that the major bioactive compounds were Δ-9- tetrahydrocannabinol (THC) and cannabidiol (CBD). While minor bioactive compounds included gallic acid and tannic acid. These results support the benefits of cannabis sugar leaf extract, which has been used for its pharmacological properties and may be useful as an alternative antimicrobial agent in medicine.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"12 1","pages":"44-52"},"PeriodicalIF":1.5000,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186785/pdf/","citationCount":"0","resultStr":"{\"title\":\"In vitro antimicrobial activity of Thai stick cannabis Hang Kra Rog Phu Phan (<i>Cannabis sativa</i> L.), sugar leaves extract against pathogenic bacteria.\",\"authors\":\"Panicha Pongnaratorn, Natthida Sophon, Parichart Boueroy\",\"doi\":\"10.5455/javar.2025.l870\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong><i>Cannabis sativa</i> L. is aware of a rich source of bioactive substances with various structures that exhibit pharmacological activity in the central nervous system, cardiovascular, cerebrovascular, respiratory, reproductive, and gastrointestinal systems.</p><p><strong>Materials and methods: </strong>In this study, cannabis sugar leaves were soaked in 99% ethanol, followed by evaporation. The antibacterial effect of the cannabis sugar leaf extract was then evaluated using the disc diffusion method. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined using broth dilution.</p><p><strong>Results: </strong>The results of this study indicated that the cannabis sugar leaf extract inhibited <i>Bacillus cereus</i>, <i>Vibrio cholerae</i>, <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, and <i>Staphylococcus epidermidis</i> when compared to tetracycline, but it did not inhibit <i>Pseudomonas</i> <i>aeruginosa</i>. The MIC and MBC of the cannabis sugar leaves extract against <i>B</i>. <i>cereus</i>, <i>V</i>. <i>cholerae</i>, <i>E</i>. <i>coli</i>, <i>S</i>. <i>aureus</i>, and <i>S</i>. <i>epidermidis</i> were 0.977, 1.953, 31.25, 62.5, 125, 250, 250, 500, 250, and 500 mg/ml, respectively. The bioactive compounds in cannabis sugar leaf extract were identified using high-performance liquid chromatography.</p><p><strong>Conclusion: </strong>The results indicated that the major bioactive compounds were Δ-9- tetrahydrocannabinol (THC) and cannabidiol (CBD). While minor bioactive compounds included gallic acid and tannic acid. These results support the benefits of cannabis sugar leaf extract, which has been used for its pharmacological properties and may be useful as an alternative antimicrobial agent in medicine.</p>\",\"PeriodicalId\":14892,\"journal\":{\"name\":\"Journal of Advanced Veterinary and Animal Research\",\"volume\":\"12 1\",\"pages\":\"44-52\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2025-03-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186785/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Advanced Veterinary and Animal Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5455/javar.2025.l870\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/3/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Advanced Veterinary and Animal Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5455/javar.2025.l870","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/3/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
In vitro antimicrobial activity of Thai stick cannabis Hang Kra Rog Phu Phan (Cannabis sativa L.), sugar leaves extract against pathogenic bacteria.
Objective: Cannabis sativa L. is aware of a rich source of bioactive substances with various structures that exhibit pharmacological activity in the central nervous system, cardiovascular, cerebrovascular, respiratory, reproductive, and gastrointestinal systems.
Materials and methods: In this study, cannabis sugar leaves were soaked in 99% ethanol, followed by evaporation. The antibacterial effect of the cannabis sugar leaf extract was then evaluated using the disc diffusion method. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined using broth dilution.
Results: The results of this study indicated that the cannabis sugar leaf extract inhibited Bacillus cereus, Vibrio cholerae, Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis when compared to tetracycline, but it did not inhibit Pseudomonasaeruginosa. The MIC and MBC of the cannabis sugar leaves extract against B. cereus, V. cholerae, E. coli, S. aureus, and S. epidermidis were 0.977, 1.953, 31.25, 62.5, 125, 250, 250, 500, 250, and 500 mg/ml, respectively. The bioactive compounds in cannabis sugar leaf extract were identified using high-performance liquid chromatography.
Conclusion: The results indicated that the major bioactive compounds were Δ-9- tetrahydrocannabinol (THC) and cannabidiol (CBD). While minor bioactive compounds included gallic acid and tannic acid. These results support the benefits of cannabis sugar leaf extract, which has been used for its pharmacological properties and may be useful as an alternative antimicrobial agent in medicine.
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