游离和封装DNA示踪剂在立陶宛气候条件下地表水研究中的应用。

IF 4.8 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomolecules Pub Date : 2025-06-18 DOI:10.3390/biom15060889

Dominyka Švedaitė, Anastasija Kriučkova, Augustas Morkvėnas, Vitalijus Karabanovas, Gintautas Stankūnavičius, Vigilija Klima, Jaunius Urbonavičius, Rūta Ivanec-Goranina

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引用次数: 0

摘要

评价了游离和封装DNA作为示踪剂在立陶宛气候条件下地表水研究中的适用性。采用实时聚合酶链反应（RT-PCR）进行示踪DNA合成和分析。用海藻酸盐和壳聚糖制备DNA微胶囊，用原子力显微镜测定微胶囊的大小。选择维尔纽斯市的穆尔勒河进行实地实验，使用制备的示踪剂。结果表明，两种示踪剂均可应用于地表水研究，但封装DNA示踪剂的相对浓度回收率是游离DNA示踪剂的3-6倍。综上所述，藻酸盐/壳聚糖胶囊可以保护DNA免受murlvili河流砂层、直接紫外线照射和其他环境因素的降解。据我们所知，这是关于游离和封装DNA示踪剂在立陶宛地表水研究中的应用的第一份报告。

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Application of Free and Encapsulated DNA Tracers in Surface Water Studies in Lithuanian Climatic Conditions.

The applicability of free and encapsulated DNA as tracers in surface water studies in Lithuanian climatic conditions was evaluated. Tracer DNA synthesis and analysis were performed using real-time polymerase chain reaction (RT-PCR). Alginate and chitosan were used to obtain the microcapsules with DNA, and their sizes were determined using an atomic force microscopy. The Murlė stream in the city of Vilnius was chosen for field experiments using the prepared tracers. It was found that both types of tracers may be applied to surface water studies, but the relative concentration recovery of encapsulated DNA tracers is 3-6 times higher than that of free DNA tracers. It was concluded that the alginate/chitosan capsules protect DNA from the sandy layer in Murlė stream, direct UV exposure and other environmental factors that could degrade DNA. To our knowledge, this is the first report about free and encapsulated DNA tracer application in surface water studies in Lithuania.

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来源期刊

Biomolecules Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

9.40

自引率

3.60%

发文量

1640

审稿时长

18.28 days

期刊介绍： Biomolecules (ISSN 2218-273X) is an international, peer-reviewed open access journal focusing on biogenic substances and their biological functions, structures, interactions with other molecules, and their microenvironment as well as biological systems. Biomolecules publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.