MhNF-YA1-Like-MhDnaJC76-MhAAO3转录级联增强苹果ABA生物合成和抗旱性

IF 6 1区 生物学 Q1 PLANT SCIENCES
Jianwen Tian, Chunhui Song, Xiang Gao, Juan Gong, Lulu Yu, Xinwu Tian, Kunxi Zhang, Jian Jiao, Miaomiao Wang, Pengbo Hao, Xianbo Zheng, Tuanhui Bai
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引用次数: 0

摘要

干旱作为非生物胁迫中最不利和最普遍的环境因子,已成为严重影响作物生长和产量的重大全球性问题。核因子- y (NF-Y)转录因子在植物抗逆性调控中的作用已得到充分证实。然而,NF-YS在赋予苹果抗旱性中的具体功能和分子机制在很大程度上仍未被探索。在此,我们报道了NF-Y转录因子MhNF-YA1-like正向调节苹果(Malus domestica Borkh.)的抗旱性。MhNF-YA1-like在干旱和ABA处理下转录上调。过表达MhNF-YA1-like可以增强苹果的耐旱性,而沉默则导致耐旱性减弱。进一步研究发现MhNF-YA1-like直接结合MhAAO3的启动子并激活其表达。此外,我们发现MhDnaJC76是MhNF-YA1的相互作用蛋白,MhDnaJC76正调节苹果的抗旱性。此外,MhNF-YA1-like与MhDnaJC76的互作显著增强了MhNF-YA1-like对MhAAO3的调控作用,从而促进ABA的积累,从而提高苹果的抗旱性。综上所述,我们的研究结果阐明了MhNF-YA1-like-MhDnaJC76复合物调节苹果耐旱性的分子框架,从而为开发耐旱作物提供了靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
MhNF-YA1-Like-MhDnaJC76-MhAAO3 Transcriptional Cascade Enhances ABA Biosynthesis and Drought Tolerance in Apple.

Drought, the most unfavorable and widespread environmental factor among abiotic stresses, has become a significant global issue that severely crop growth and yield. Nuclear factor-Y (NF-Y) transcription factors have well-documented functions in regulating stress tolerance in plants. However, the specific functions and molecular mechanisms of NF-YS in conferring drought tolerance in apples remain largely unexplored. Here, we reported that the NF-Y transcription factor MhNF-YA1-like positively modulates drought stress tolerance in apple (Malus domestica Borkh.). MhNF-YA1-like is transcriptionally upregulated in response to drought and abscisic acid (ABA) treatments. Overexpression of MhNF-YA1-like enhances drought tolerance in apples, whereas its silencing results in weakened drought tolerance. Further investigation reveals that MhNF-YA1-like directly binds to the promoter of MhAAO3 and activates its expression. Additionally, we identified MhDnaJC76 as an interacting protein of MhNF-YA1, and MhDnaJC76 positively modulates apple drought tolerance. Furthermore, the interaction between MhNF-YA1-like and MhDnaJC76 significantly amplifies the regulatory effect of MhNF-YA1-like on MhAAO3, thereby promoting ABA accumulation and consequently enhancing apple drought tolerance. Taken together, our findings elucidate a molecular framework by which the MhNF-YA1-like-MhDnaJC76 complex modulates drought tolerance in apples, thereby providing targets for developing drought-tolerant crops.

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来源期刊
Plant, Cell & Environment
Plant, Cell & Environment 生物-植物科学
CiteScore
13.30
自引率
4.10%
发文量
253
审稿时长
1.8 months
期刊介绍: Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.
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