促进角膜再上皮形成的生物粘附功能肽衍生的超分子水凝胶。

IF 5.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules Pub Date : 2025-06-26 DOI:10.1021/acs.biomac.5c00221

Lei Lei, Jia Liu, Yueting Shi, Yiping Wu, Wei Wu, Yuhan Hu, Haoxuan Wang, Jiaqing Wang, Xingyi Li

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引用次数: 0

摘要

损伤后角膜的快速再上皮化对维持角膜稳态和屏障功能至关重要。本研究报道了一种促进角膜再上皮形成的生物粘附功能肽衍生的超分子水凝胶（FBA-FFGGRGD）。醛修饰的生物活性肽（RGD）使水凝胶具有生物粘附特性，可通过希夫碱反应与角膜基底膜蛋白进行共价锚定。与类似物TA-FFGGRGD相比，FBA-FFGGRGD显著增强人角膜上皮细胞（HCECs）的粘附、迁移和增殖，48 h内细胞数量增加一倍，上调occluden -1 （ZO-1）表达。体内研究表明，在兔角膜损伤模型中，水凝胶具有良好的眼生物相容性，延长角膜前保留时间（约40分钟），并促进分层上皮的形成。通过重塑上皮屏障，这种生物黏附水凝胶为维持角膜稳态和预防眼部疾病提供了一种很有前途的策略。

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A Bioadhesive Functional Peptide-Derived Supramolecular Hydrogel for the Promotion of Corneal Re-Epithelization.

The rapid corneal re-epithelization after injury is crucial for maintaining corneal homeostasis and barrier function. This study reports a bioadhesive functional peptide-derived supramolecular hydrogel (FBA-FFGGRGD) for promoting corneal re-epithelization. The aldehyde-modified bioactive peptide (RGD) endows the hydrogel with bioadhesive properties, enabling covalent anchoring to corneal basement membrane proteins via Schiff base reaction. Compared with its analogue (TA-FFGGRGD), FBA-FFGGRGD significantly enhances adhesion, migration, and proliferation of human corneal epithelial cells (HCECs), doubling cell numbers within 48 h and upregulating zonula occludens-1 (ZO-1) expression. In vivo studies show that the hydrogel exhibits excellent ocular biocompatibility, prolongs precorneal retention (∼40 min), and promotes stratified epithelium formation in a rabbit model of corneal injury. By remodeling the epithelial barrier, this bioadhesive hydrogel provides a promising strategy for corneal homeostasis preservation and ocular disorder prevention.

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来源期刊

Biomacromolecules 化学-高分子科学

CiteScore

10.60

自引率

4.80%

发文量

417

审稿时长

1.6 months

期刊介绍： Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine. Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.