lncRNA PXN- as1 - l表达MBNL3和PXN基因对胃癌的促进作用

IF 2.2 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2025-06-27 DOI:10.1016/j.bbrep.2025.102117

Mai A.H. Abouelenin , Amany A. Saleh , Naglaa S. Elabd , Osama Elbahr , Reham Ahmed Abdelaziz , Mohammed G. Elhelbawy , Zeinab A. Kasemy , Ahmed Abdelkhalek Hussein , Mona A. Abbas

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引用次数: 0

摘要

胃癌的发病率和死亡率均居世界第四位。几个基因影响它的发生和发展。材料与方法采用RT-PCR方法检测75例胃癌患者和75例对照组胃组织中MBNL3、PXN基因及lncRNA PXN- as1 - l的表达。结果胃癌组织中smbnl3、PXN和lncRNA PXN- as1 - l的表达明显高于邻近正常组织和对照正常组织(p <；0.001)。LncRNA PXN- as1 - l和MBNL3的敏感性最高（分别为92.00 %和90.67%），其中PXN对癌组织与邻近正常组织鉴别的敏感性为81.33%，而PXN和LncRNA PXN- as1 - l作为胃癌组织与对照组正常组织鉴别的敏感性最高（分别为98.67%和92.00 %），MBNL3的敏感性为89.33%。在转移性和非转移性患者中，MBNL3和PXN是最高的鉴别标志物（敏感性分别为87.50%和83.33%）。MBNL3、PXN和lncRNA PXN- as1 - l是胃癌的可靠预测因子（分别为p = 0.004、p = 0.009和p = 0.001），但只有MBNL3和lncRNA PXN- as1 - l是死亡率的显著预测因子（分别为p = 0.026和p = 0.043）。升高的表达与较低的总生存率和无进展生存率有关。结论mbnl3、PXN和lncRNA PXN- as1 - l表达水平可用于胃癌的诊断和预测，并可区分转移和非转移患者。MBNL3和lncRNA PXN-AS1-L的表达水平可以预测胃癌死亡率。MBNL3和PXN的高表达与侵袭性胃癌、低总生存率和无进展生存率有关。

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Promotive role of MBNL3 and PXN genes expressions with lncRNA PXN-AS1-L on gastric cancer

Background

Gastric cancer ranks fourth in both incidence and mortality worldwide. Several genes influence its genesis and progress.

Materials and methods

The expression of MBNL3, PXN genes, and lncRNA PXN-AS1-L was evaluated in gastric tissue samples from 75 gastric cancer patients and 75 controls by RT-PCR.

Results

MBNL3, PXN, and lncRNA PXN-AS1-L expressions were considerably raised among cancerous gastric tissue than in nearby normal tissue and normal tissue from controls (p < 0.001). LncRNA PXN-AS1-L and MBNL3 had the highest sensitivity (92.00 % and 90.67 %, respectively), with PXN sensitivity of 81.33 % for differentiation of the cancer tissue from adjacent normal tissue, while PXN and lncRNA PXN-AS1-L had the highest sensitivity (98.67 % and 92.00 %, respectively), with 89.33 % for MBNL3 as discriminators between gastric cancer tissue and normal tissue from controls. Between metastatic and non-metastatic patients, MBNL3 and PXN were the highest differentiating markers (sensitivity = 87.50 % and 83.33 %, respectively). MBNL3, PXN, and lncRNA PXN-AS1-L were reliable predictors of gastric cancer (p = 0.004, p = 0.009, and p = 0.001, respectively), but only MBNL3 and lncRNA PXN-AS1-L were significant predictors for mortality (p = 0.026 and p = 0.043, respectively). The elevated expressions were linked to lower overall and progression-free survival rates.

Conclusion

MBNL3, PXN, and lncRNA PXN-AS1-L expression levels can diagnose and predict gastric cancer and discriminate between metastatic and non-metastatic patients. The expression level of MBNL3 and lncRNA PXN-AS1-L can predict gastric cancer mortality. Higher expression of MBNL3 and PXN was linked to aggressive gastric cancer and low overall and progression-free survival rates.

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.