分泌蛋白PgSLP与柑橘钙调素样蛋白CsCML27/44在果实病害防御中的相互作用

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2025-06-27 DOI:10.1016/j.postharvbio.2025.113757

Yao Xu , Ou Chen , Rong Zhu , Rui Huang , Jian Ming , Shixiang Yao , Kaifang Zeng

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引用次数: 0

摘要

蓝霉病是柑桔果实最重要的采后病害之一，造成重大的经济损失。生物防治酵母通过分泌蛋白质增强果实的抗性。先前的研究表明，瞬时过表达毕氏毕氏酵母分泌蛋白PgSLP可降低柑橘果实中蓝霉病的发病率。然而，分子机制尚不清楚。本研究探讨了Ca 2 +在pgslp诱导的耐药机制中的作用，并探索了其与钙调素样蛋白（cml）的相互作用。结果表明，钙螯合剂抑制了pgslp诱导的耐药性，表明ca2 +在其中起着至关重要的作用。PgSLP的瞬时过表达上调了几种Ca 2 +相关基因，包括cml、环核苷酸门控通道（CNGCs）、钙依赖性蛋白激酶（CDPKs）和谷氨酸受体样通道（GLRs）。AlphaFold3预测揭示了PgSLP和cscml之间的相互作用。酵母双杂交（Y2H）实验证实了PgSLP与CsCML27和CsCML44之间的相互作用，并利用双分子荧光互补（BiFC）和荧光素酶互补成像（LCI）对其进行了验证。此外，PgSLP与CsCML27或CsCML44的瞬时过表达进一步增强了果实对蓝霉病的抗性。这些结果强调了Ca2 +信号的重要性，并为生物防治酵母介导的蓝霉菌抗性背后的过程提供了新的视角。

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Interaction of secreted protein PgSLP and citrus calmodulin-like protein CsCML27/44 in fruit disease defense

Blue mold is one of the most important postharvest diseases of citrus fruit, causing significant economic losses. Biocontrol yeasts enhance fruit resistance through secreted proteins. Previous studies demonstrated that transient overexpression of Pichia galeiformis secreted protein PgSLP reduces blue mold incidence in citrus fruit. However, the molecular mechanisms remain unclear. This study investigates the role of Ca²⁺ in PgSLP-induced resistance mechanism and explores its interaction with calmodulin-like proteins (CMLs). The results show that the calcium chelator inhibits PgSLP-induced resistance, indicating a crucial role for Ca²⁺. Transient overexpression of PgSLP upregulated several Ca²⁺-related genes, including CMLs, cyclic nucleotide-gated channels (CNGCs), calcium-dependent protein kinases (CDPKs), and glutamate receptor-like channels (GLRs). AlphaFold3 predictions revealed interactions between PgSLP and CsCMLs. Yeast two-hybrid (Y2H) assays confirmed interactions between PgSLP and CsCML27 and CsCML44, which were validated using bimolecular fluorescence complementation (BiFC) and luciferase complementation imaging (LCI). Furthermore, combined transient overexpression of PgSLP with either CsCML27 or CsCML44 further enhanced fruit resistance to blue mold. These results emphasize the significance of Ca^2 + signaling and offer fresh perspectives on the processes behind biocontrol yeast-mediated resistance to blue mold.

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.