植物蛋白和乳蛋白混合加工奶酪的物理特性和微观结构

IF 11 1区 农林科学 Q1 CHEMISTRY, APPLIED
Di Lu , Debashree Roy , Alejandra Acevedo-Fani , Harjinder Singh , Mark Waterland , Aiqian Ye
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引用次数: 0

摘要

制备了含有不同比例的绿豆(MPI)或大麻蛋白(HPI)和凝乳酶酪蛋白(RC)的混合加工奶酪类似物(HPCAs),并对其进行了分析,以了解其空间和微观结构分布以及相关的物理性质,如流变性能、质地轮廓、熔融性和拉伸性。此外,利用SDS-PAGE和FTIR光谱研究了蛋白质组成和二级蛋白结构,利用CLSM和TEM观察了奶酪基质的微观结构。结果表明,植物蛋白的种类和浓度对hpca的物理性质和微观结构有显著影响。添加30%或更多的植物蛋白改变了奶酪类似物的物理和质地特性以及微观结构,β-薄片含量减少,随机线圈结构增加。与相同混合比例的大麻蛋白系统相比,绿豆蛋白基hpca表现出更大的拉伸性(例如,30% MPI为93.8 mm, 30% HPI为41.53 mm)、流变学和纹理性能,但不具有熔融性(例如,70% MPI为1%,70% HPI为48%)。这种差异可归因于植物蛋白聚集的大小。所有数据均采用Tukey检验的单因素方差分析(p <;0.05)。这些发现加深了我们对植物蛋白奶酪和杂交奶酪的理解,为优化植物性乳制品替代品铺平了道路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Physical properties and microstructure of hybrid processed cheeses formulated with plant protein and milk protein ingredients

Physical properties and microstructure of hybrid processed cheeses formulated with plant protein and milk protein ingredients
Hybrid processed cheese analogues (HPCAs) containing either mung bean (MPI) or hemp protein (HPI) with rennet casein (RC) at various ratios were prepared and analysed to understand their spatial and microstructural distribution and related physical properties, such as rheological properties, texture profile, meltability, and stretchability. In addition, protein composition and secondary protein structure were studied using SDS–PAGE and FTIR spectroscopy, while CLSM and TEM were employed to visualise the microstructure of the cheese matrix. Results indicated that plant protein types and concentration significantly affected the physical properties and microstructure of HPCAs. The addition of 30 % or more plant protein altered the physical and textural properties as well as the microstructure of the cheese analogues, with a decrease in β-sheet content and an increase in random coil structures. Mung bean protein–based HPCAs exhibit greater stretchability (e.g. 93.8 mm in 30 % MPI vs 41.53 mm in 30 % HPI), rheological, and textural properties, but not meltability (e.g. 1 % in 70 % MPI vs 48 % in 70 % HPI), compared with the hemp protein system at the same mixing ratios. This difference can be attributed to the size of the plant protein aggregation. All data were analysed by one-way ANOVA with Tukey's test (p < 0.05). These findings deepen our understanding of plant protein-based and hybrid cheeses, paving the way for optimised plant-based dairy alternatives.
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来源期刊
Food Hydrocolloids
Food Hydrocolloids 工程技术-食品科技
CiteScore
19.90
自引率
14.00%
发文量
871
审稿时长
37 days
期刊介绍: Food Hydrocolloids publishes original and innovative research focused on the characterization, functional properties, and applications of hydrocolloid materials used in food products. These hydrocolloids, defined as polysaccharides and proteins of commercial importance, are added to control aspects such as texture, stability, rheology, and sensory properties. The research's primary emphasis should be on the hydrocolloids themselves, with thorough descriptions of their source, nature, and physicochemical characteristics. Manuscripts are expected to clearly outline specific aims and objectives, include a fundamental discussion of research findings at the molecular level, and address the significance of the results. Studies on hydrocolloids in complex formulations should concentrate on their overall properties and mechanisms of action, while simple formulation development studies may not be considered for publication. The main areas of interest are: -Chemical and physicochemical characterisation Thermal properties including glass transitions and conformational changes- Rheological properties including viscosity, viscoelastic properties and gelation behaviour- The influence on organoleptic properties- Interfacial properties including stabilisation of dispersions, emulsions and foams- Film forming properties with application to edible films and active packaging- Encapsulation and controlled release of active compounds- The influence on health including their role as dietary fibre- Manipulation of hydrocolloid structure and functionality through chemical, biochemical and physical processes- New hydrocolloids and hydrocolloid sources of commercial potential. The Journal also publishes Review articles that provide an overview of the latest developments in topics of specific interest to researchers in this field of activity.
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