Shaobo Guo, Murtala Bindawa Isah, Ruiling Hu, Zhongshang Guo, Xiaodan Wei, Zhifeng Liu, Xiaohui Ji*, Alberto C.P. Dias and Xiaoying Zhang*,
{"title":"金属基异质结在体外和体内控制多种阳离子和活性氧释放抑制多重耐药细菌。","authors":"Shaobo Guo, Murtala Bindawa Isah, Ruiling Hu, Zhongshang Guo, Xiaodan Wei, Zhifeng Liu, Xiaohui Ji*, Alberto C.P. Dias and Xiaoying Zhang*, ","doi":"10.1021/acsami.5c05798","DOIUrl":null,"url":null,"abstract":"<p >Spherical heterojunction nanocomposite materials are utilized to treat wound infections caused by drug-resistant bacteria by generating reactive oxygen species (ROS) and multiple cations (multiple inorganic or organic ions with positive points). However, there is an ongoing debate on the relative contributions of ROS and multiple cations toward antibacterial activity. In this study, the CuFe<sub>2</sub>O<sub>4</sub>/Cu@PEI/Ag (ZPA) nanocomposites were synthesized for releasing abundant<sup>•</sup>O<sup>2–</sup>,<sup>•</sup>OH, Fe<sup>3+</sup>, Cu<sup>2+</sup>, Ag<sup>+</sup>, and polyethylenimine (PEI), and studied the contribution of the released ions to the bacteriostatic activity against drug sensitive <i>Staphylococcus aureus</i> (ATCC25923) and drug-resistant <i>S. aureus</i> (ATCC43360). The results revealed that the antibacterial activity is attributed in the following order: multiple cations ><sup>•</sup>O<sup>2–</sup>><sup>•</sup>OH > single cation. The antibacterial mechanism of the material involved leakage of the cytoplasmic content by damaging the bacterial cell wall, and the alteration of the secondary structure of the cell wall by multiple cations bound to the bacterial cell wall via electrostatic attraction. By healing drug-resistant <i>S. aureus</i>-induced wound infection and completely eliminating bacterial burden after 11 days, in addition, ZPA also effectively polarized M1 type macrophages to M2 type in vivo to promote wound healing. Thus, our findings elucidate that multiple cations occupy an important position on the antibacterial properties of composite nanomaterials. Moreover, The ZPA represent a promising strategy for addressing drug-resistant <i>S. aureus</i>-induced wound infections.</p>","PeriodicalId":5,"journal":{"name":"ACS Applied Materials & Interfaces","volume":"17 27","pages":"38859–38873"},"PeriodicalIF":8.2000,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Metal-Based Heterojunction for Controlled Release of Multiple Cations and Reactive Oxygen Species Inhibiting Multidrug-Resistant Bacteria In Vitro and In Vivo\",\"authors\":\"Shaobo Guo, Murtala Bindawa Isah, Ruiling Hu, Zhongshang Guo, Xiaodan Wei, Zhifeng Liu, Xiaohui Ji*, Alberto C.P. Dias and Xiaoying Zhang*, \",\"doi\":\"10.1021/acsami.5c05798\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Spherical heterojunction nanocomposite materials are utilized to treat wound infections caused by drug-resistant bacteria by generating reactive oxygen species (ROS) and multiple cations (multiple inorganic or organic ions with positive points). However, there is an ongoing debate on the relative contributions of ROS and multiple cations toward antibacterial activity. In this study, the CuFe<sub>2</sub>O<sub>4</sub>/Cu@PEI/Ag (ZPA) nanocomposites were synthesized for releasing abundant<sup>•</sup>O<sup>2–</sup>,<sup>•</sup>OH, Fe<sup>3+</sup>, Cu<sup>2+</sup>, Ag<sup>+</sup>, and polyethylenimine (PEI), and studied the contribution of the released ions to the bacteriostatic activity against drug sensitive <i>Staphylococcus aureus</i> (ATCC25923) and drug-resistant <i>S. aureus</i> (ATCC43360). The results revealed that the antibacterial activity is attributed in the following order: multiple cations ><sup>•</sup>O<sup>2–</sup>><sup>•</sup>OH > single cation. The antibacterial mechanism of the material involved leakage of the cytoplasmic content by damaging the bacterial cell wall, and the alteration of the secondary structure of the cell wall by multiple cations bound to the bacterial cell wall via electrostatic attraction. By healing drug-resistant <i>S. aureus</i>-induced wound infection and completely eliminating bacterial burden after 11 days, in addition, ZPA also effectively polarized M1 type macrophages to M2 type in vivo to promote wound healing. Thus, our findings elucidate that multiple cations occupy an important position on the antibacterial properties of composite nanomaterials. Moreover, The ZPA represent a promising strategy for addressing drug-resistant <i>S. aureus</i>-induced wound infections.</p>\",\"PeriodicalId\":5,\"journal\":{\"name\":\"ACS Applied Materials & Interfaces\",\"volume\":\"17 27\",\"pages\":\"38859–38873\"},\"PeriodicalIF\":8.2000,\"publicationDate\":\"2025-06-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Materials & Interfaces\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acsami.5c05798\",\"RegionNum\":2,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MATERIALS SCIENCE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Materials & Interfaces","FirstCategoryId":"88","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acsami.5c05798","RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
A Metal-Based Heterojunction for Controlled Release of Multiple Cations and Reactive Oxygen Species Inhibiting Multidrug-Resistant Bacteria In Vitro and In Vivo
Spherical heterojunction nanocomposite materials are utilized to treat wound infections caused by drug-resistant bacteria by generating reactive oxygen species (ROS) and multiple cations (multiple inorganic or organic ions with positive points). However, there is an ongoing debate on the relative contributions of ROS and multiple cations toward antibacterial activity. In this study, the CuFe2O4/Cu@PEI/Ag (ZPA) nanocomposites were synthesized for releasing abundant•O2–,•OH, Fe3+, Cu2+, Ag+, and polyethylenimine (PEI), and studied the contribution of the released ions to the bacteriostatic activity against drug sensitive Staphylococcus aureus (ATCC25923) and drug-resistant S. aureus (ATCC43360). The results revealed that the antibacterial activity is attributed in the following order: multiple cations >•O2–>•OH > single cation. The antibacterial mechanism of the material involved leakage of the cytoplasmic content by damaging the bacterial cell wall, and the alteration of the secondary structure of the cell wall by multiple cations bound to the bacterial cell wall via electrostatic attraction. By healing drug-resistant S. aureus-induced wound infection and completely eliminating bacterial burden after 11 days, in addition, ZPA also effectively polarized M1 type macrophages to M2 type in vivo to promote wound healing. Thus, our findings elucidate that multiple cations occupy an important position on the antibacterial properties of composite nanomaterials. Moreover, The ZPA represent a promising strategy for addressing drug-resistant S. aureus-induced wound infections.
期刊介绍:
ACS Applied Materials & Interfaces is a leading interdisciplinary journal that brings together chemists, engineers, physicists, and biologists to explore the development and utilization of newly-discovered materials and interfacial processes for specific applications. Our journal has experienced remarkable growth since its establishment in 2009, both in terms of the number of articles published and the impact of the research showcased. We are proud to foster a truly global community, with the majority of published articles originating from outside the United States, reflecting the rapid growth of applied research worldwide.