ERF5-WOX4转录因子调控模块控制铁皮石斛甘露聚糖的生物合成。

IF 6.5 1区 生物学 Q1 PLANT SCIENCES
Danqi Zeng,Hongyu Shi,Jing Chen,Can Si,Jun Duan,Zhong-Jian Liu,Chunmei He
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引用次数: 0

摘要

植物产生甘露聚糖,其组成的一类多糖具有相当大的潜力,健康相关的应用。gdp -甘露糖焦磷酸化酶(GMP)通过促进gdp -甘露糖的形成,在甘露糖的生物合成中起着关键作用。本研究通过酵母单杂交文库筛选,确定了铁皮石斛中WUSCHEL (WUS)相关同源盒(WOX)转录因子DoWOX4作为与DoGMP1启动子相互作用的候选蛋白。我们证明了DoWOX4直接结合到DoGMP1启动子并激活其表达。过表达DoWOX4显著增强甘露聚糖的生物合成,而沉默DoWOX4则抑制这一过程。此外,我们发现乙烯反应因子5 (DoERF5)在体外与DoWOX4启动子结合。随后的分析显示,DoERF5负调控DoWOX4和甘露聚糖的生物合成。该研究加深了我们对植物甘露聚糖生物合成调控机制的认识,为植物生物育种提供了宝贵的遗传资源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The ERF5-WOX4 transcription factor regulatory module controls mannan biosynthesis in the orchid Dendrobium officinale.
Plants produce mannans, which compose a class of polysaccharides with considerable potential for health-related applications. GDP-mannose pyrophosphorylase (GMP) plays a pivotal role in mannan biosynthesis by facilitating the formation of GDP-mannose. In this study, we identified the WUSCHEL (WUS)-related homeobox (WOX) transcription factor DoWOX4 as a candidate protein that interacts with the DoGMP1 promoter in Dendrobium officinale through yeast one-hybrid library screening. We demonstrated that DoWOX4 directly binds to the DoGMP1 promoter and activates its expression. DoWOX4 overexpression significantly enhanced mannan biosynthesis, whereas silencing DoWOX4 inhibited this process. Furthermore, we found that the ethylene response factor 5 (DoERF5) binds to the DoWOX4 promoter in vitro. Subsequent analyses revealed that DoERF5 negatively regulates DoWOX4 and mannan biosynthesis. This study deepens our understanding of the regulatory mechanisms governing mannan biosynthesis in plants and provides valuable genetic resources for plant bio-breeding initiatives.
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来源期刊
Plant Physiology
Plant Physiology 生物-植物科学
CiteScore
12.20
自引率
5.40%
发文量
535
审稿时长
2.3 months
期刊介绍: Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research. As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.
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