移植新鲜和冷冻保存的自体脂肪组织在肌肉体积损失重建中改善新形成肌肉组织的再整合现象:组织学评估。

IF 2.1
Irene Cantarero, Maria A Peña-Toledo, Maria E Lopez-Espejo, Maria Jesús Gil-Belmonte, Rafael Villalba, Ignacio Jimena, José Peña-Amaro
{"title":"移植新鲜和冷冻保存的自体脂肪组织在肌肉体积损失重建中改善新形成肌肉组织的再整合现象:组织学评估。","authors":"Irene Cantarero, Maria A Peña-Toledo, Maria E Lopez-Espejo, Maria Jesús Gil-Belmonte, Rafael Villalba, Ignacio Jimena, José Peña-Amaro","doi":"10.1002/ar.70010","DOIUrl":null,"url":null,"abstract":"<p><p>Repair of volumetric muscle loss (VML) lesions tends to be poor, leading to fibrosis and functional loss. Here we investigated the effectiveness of fresh versus cryopreserved autologous adipose tissue transplantation to facilitate repair. Wistar rats were distributed into four experimental groups: normal control, VML lesions, VML lesions treated with a fresh autologous adipose tissue graft, and VML treated with a cryopreserved autologous adipose tissue graft. At 60 days post-intervention, a histological, histochemical, and immunohistochemical analysis was performed to assess changes related to the degree of fibrosis, vascularization, and innervation. These changes were quantified and statistically analyzed. The changes generated by the two types of adipose tissue implanted in the VML lesion were highly similar, with some significant differences favoring the use of cryopreserved adipose tissue. Compared with the VML group, the following outcomes were observed: (1) significant presence of regenerated muscle fibers; (2) significant reduction of fibrosis, albeit with a higher proportion of type III collagen; (3) significant increase in the microvascular pattern; and (4) significant reduction in the number of angulated atrophic muscle fibers and increase in cytoarchitectural changes indicative of reinnervation. This study suggests that autologous adipose tissue transplantation in VML lesions promotes reintegrative processes, facilitating the regeneration and formation of new muscle fibers. Furthermore, the freezing did not diminish outcomes in the CAAT group, highlighting its potential for preservation in tissue banks and applications in regenerative medicine.</p>","PeriodicalId":520555,"journal":{"name":"Anatomical record (Hoboken, N.J. : 2007)","volume":" ","pages":""},"PeriodicalIF":2.1000,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Transplants of fresh and cryopreserved autologous adipose tissue improve reintegrative phenomena in newly formed muscle tissue in the reconstruction of muscle volume loss: A histological evaluation.\",\"authors\":\"Irene Cantarero, Maria A Peña-Toledo, Maria E Lopez-Espejo, Maria Jesús Gil-Belmonte, Rafael Villalba, Ignacio Jimena, José Peña-Amaro\",\"doi\":\"10.1002/ar.70010\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Repair of volumetric muscle loss (VML) lesions tends to be poor, leading to fibrosis and functional loss. Here we investigated the effectiveness of fresh versus cryopreserved autologous adipose tissue transplantation to facilitate repair. Wistar rats were distributed into four experimental groups: normal control, VML lesions, VML lesions treated with a fresh autologous adipose tissue graft, and VML treated with a cryopreserved autologous adipose tissue graft. At 60 days post-intervention, a histological, histochemical, and immunohistochemical analysis was performed to assess changes related to the degree of fibrosis, vascularization, and innervation. These changes were quantified and statistically analyzed. The changes generated by the two types of adipose tissue implanted in the VML lesion were highly similar, with some significant differences favoring the use of cryopreserved adipose tissue. Compared with the VML group, the following outcomes were observed: (1) significant presence of regenerated muscle fibers; (2) significant reduction of fibrosis, albeit with a higher proportion of type III collagen; (3) significant increase in the microvascular pattern; and (4) significant reduction in the number of angulated atrophic muscle fibers and increase in cytoarchitectural changes indicative of reinnervation. This study suggests that autologous adipose tissue transplantation in VML lesions promotes reintegrative processes, facilitating the regeneration and formation of new muscle fibers. Furthermore, the freezing did not diminish outcomes in the CAAT group, highlighting its potential for preservation in tissue banks and applications in regenerative medicine.</p>\",\"PeriodicalId\":520555,\"journal\":{\"name\":\"Anatomical record (Hoboken, N.J. : 2007)\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2025-06-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Anatomical record (Hoboken, N.J. : 2007)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1002/ar.70010\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Anatomical record (Hoboken, N.J. : 2007)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/ar.70010","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

体积性肌肉损失(VML)病变的修复往往较差,导致纤维化和功能丧失。在这里,我们研究了新鲜与冷冻保存的自体脂肪组织移植促进修复的有效性。Wistar大鼠分为4个实验组:正常对照组、VML病变组、新鲜自体脂肪组织移植治疗VML病变组和冷冻保存的自体脂肪组织移植治疗VML组。干预后60天,进行组织学、组织化学和免疫组织化学分析,以评估与纤维化、血管化和神经支配程度相关的变化。对这些变化进行量化和统计分析。两种脂肪组织植入VML病变后产生的变化高度相似,有一些明显的差异,有利于使用冷冻保存的脂肪组织。与VML组比较,观察到:(1)肌纤维再生明显;(2)纤维化明显减少,尽管III型胶原比例较高;(3)微血管形态明显增加;(4)成角萎缩肌纤维数量显著减少,细胞结构变化增加,表明神经再生。本研究表明,自体脂肪组织移植在VML病变促进再整合过程,促进再生和新肌纤维的形成。此外,冷冻并没有降低CAAT组的结果,突出了其在组织库中保存和再生医学应用的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Transplants of fresh and cryopreserved autologous adipose tissue improve reintegrative phenomena in newly formed muscle tissue in the reconstruction of muscle volume loss: A histological evaluation.

Repair of volumetric muscle loss (VML) lesions tends to be poor, leading to fibrosis and functional loss. Here we investigated the effectiveness of fresh versus cryopreserved autologous adipose tissue transplantation to facilitate repair. Wistar rats were distributed into four experimental groups: normal control, VML lesions, VML lesions treated with a fresh autologous adipose tissue graft, and VML treated with a cryopreserved autologous adipose tissue graft. At 60 days post-intervention, a histological, histochemical, and immunohistochemical analysis was performed to assess changes related to the degree of fibrosis, vascularization, and innervation. These changes were quantified and statistically analyzed. The changes generated by the two types of adipose tissue implanted in the VML lesion were highly similar, with some significant differences favoring the use of cryopreserved adipose tissue. Compared with the VML group, the following outcomes were observed: (1) significant presence of regenerated muscle fibers; (2) significant reduction of fibrosis, albeit with a higher proportion of type III collagen; (3) significant increase in the microvascular pattern; and (4) significant reduction in the number of angulated atrophic muscle fibers and increase in cytoarchitectural changes indicative of reinnervation. This study suggests that autologous adipose tissue transplantation in VML lesions promotes reintegrative processes, facilitating the regeneration and formation of new muscle fibers. Furthermore, the freezing did not diminish outcomes in the CAAT group, highlighting its potential for preservation in tissue banks and applications in regenerative medicine.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信