无刺蜂（Melipona quadrifasciata） mirna的研究。

IF 3.6 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Non-Coding RNA Pub Date : 2025-06-19 DOI:10.3390/ncrna11030048

Dalliane Oliveira Soares, Lucas Yago Melo Ferreira, Gabriel Victor Pina Rodrigues, João Pedro Nunes Santos, Ícaro Santos Lopes, Lucas Barbosa de Amorim Conceição, Tatyana Chagas Moura, Isaque João da Silva de Faria, Roenick Proveti Olmo, Weyder Cristiano Santana, Marco Antônio Costa, Eric Roberto Guimarães Rocha Aguiar

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引用次数: 0

摘要

MicroRNAs （miRNAs）是昆虫中广泛参与生物过程的关键转录后调控因子，但对其在无刺蜜蜂中的作用知之甚少。在这里，我们首次使用小rna （sRNAs）深度测序对四趾鼠的miRNAs进行了表征。共鉴定出193个高置信度的成熟mirna，包括106个M. quadrifasciata独有序列。表达谱显示，mqu-miR-1和mqu-miR-276共同占所有miRNA读取量的70%以上，表明它们在发育和繁殖中起着核心作用。比较分析表明，四翅甲蜜蜂的mirna与其他膜翅目昆虫，尤其是蜜蜂和Bombus spp的mirna具有较高的保守性。这项工作代表了首次使用sRNAs对无刺蜜蜂的miRNA库进行全面鉴定，为了解这种生态和经济上重要的传粉媒介中miRNA介导的基因调控提供了有价值的基础。

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Insights into miRNAs of the Stingless Bee Melipona quadrifasciata.

MicroRNAs (miRNAs) are key post-transcriptional regulators involved in a wide range of biological processes in insects, yet little is known about their roles in stingless bees. Here, we present the first characterization of miRNAs in Melipona quadrifasciata using small RNAs (sRNAs) deep sequencing. A total of 193 high-confidence mature miRNAs were identified, including 106 M. quadrifasciata-exclusive sequences. Expression profiling revealed that mqu-miR-1 and mqu-miR-276 together accounted for over 70% of all miRNA reads, suggesting their central roles in development and reproduction. Comparative analyses showed a higher conservation of M. quadrifasciata miRNAs with other Hymenopterans, especially Apis mellifera and Bombus spp. Putative target genes were predicted using a consensus approach, and functional annotation indicated their involvement in diverse biological regulatory pathways. This work represents the first comprehensive identification of the miRNA repertoire in stingless bees using sRNAs and provides a valuable foundation for understanding miRNA-mediated gene regulation in this ecologically and economically important pollinator.

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来源期刊

Non-Coding RNA Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

6.70

自引率

4.70%

发文量

审稿时长

10 weeks

期刊介绍： Functional studies dealing with identification, structure-function relationships or biological activity of: small regulatory RNAs (miRNAs, siRNAs and piRNAs) associated with the RNA interference pathway small nuclear RNAs, small nucleolar and tRNAs derived small RNAs other types of small RNAs, such as those associated with splice junctions and transcription start sites long non-coding RNAs, including antisense RNAs, long ''intergenic'' RNAs, intronic RNAs and ''enhancer'' RNAs other classes of RNAs such as vault RNAs, scaRNAs, circular RNAs, 7SL RNAs, telomeric and centromeric RNAs regulatory functions of mRNAs and UTR-derived RNAs catalytic and allosteric (riboswitch) RNAs viral, transposon and repeat-derived RNAs bacterial regulatory RNAs, including CRISPR RNAS Analysis of RNA processing, RNA binding proteins, RNA signaling and RNA interaction pathways: DICER AGO, PIWI and PIWI-like proteins other classes of RNA binding and RNA transport proteins RNA interactions with chromatin-modifying complexes RNA interactions with DNA and other RNAs the role of RNA in the formation and function of specialized subnuclear organelles and other aspects of cell biology intercellular and intergenerational RNA signaling RNA processing structure-function relationships in RNA complexes RNA analyses, informatics, tools and technologies: transcriptomic analyses and technologies development of tools and technologies for RNA biology and therapeutics Translational studies involving long and short non-coding RNAs: identification of biomarkers development of new therapies involving microRNAs and other ncRNAs clinical studies involving microRNAs and other ncRNAs.