Asha Rani Sheoran, Nita Lakra, Baljeet Singh Saharan, Annu Luhach, Yogesh K Ahlawat, Rosa Porcel, Jose M Mulet, Prabhakar Singh
{"title":"代谢组学分析揭示不同芥菜基因型中pgpr驱动的耐旱性","authors":"Asha Rani Sheoran, Nita Lakra, Baljeet Singh Saharan, Annu Luhach, Yogesh K Ahlawat, Rosa Porcel, Jose M Mulet, Prabhakar Singh","doi":"10.3390/metabo15060416","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Drought stress is a major abiotic factor limiting <i>Brassica juncea</i> productivity, resulting in significant yield reductions. Plant Growth-Promoting Rhizobacteria (PGPR) have shown potential in enhancing drought tolerance; however, the metabolomic changes associated with their effects remain largely unexplored. This study examines the metabolic changes induced by a PGPR consortium (<i>Enterobacter hormaechei</i>, <i>Pantoea dispersa</i>, and <i>Acinetobacter</i> sp.) in two contrasting genotypes <i>B. juncea</i> (L.) Czern. 'RH 725' (drought tolerant) and <i>B. juncea</i> (L.) Czern. 'RH-749' (drought sensitive for drought tolerance, under both control and drought conditions.</p><p><strong>Methods: </strong>Metabolite profiling was conducted using gas chromatography-mass spectrometry (GC-MS) to identify compounds that accumulated differentially across treatments. We applied multivariate statistical methods, such as Partial Least Squares Discriminant Analysis (PLS-DA), hierarchical clustering, and pathway enrichment analysis, to explore metabolic reprogramming.</p><p><strong>Results: </strong>Drought stress induced significant changes in metabolite profile, particularly increasing the levels of osmoprotectants such as trehalose, glucose, sucrose, proline, and valine. Additionally, alterations in organic acids (malic acid and citric acid) and fatty acids (oleic acid and linoleic acid) were observed. PGPR inoculation further amplified these metabolic responses to enhance the osmotic regulation, reactive oxygen species (ROS) detoxification, and carbon-nitrogen metabolism, with RH-725 displaying a stronger adaptive response. Pathway enrichment analysis revealed that PGPR treatment significantly influenced metabolic pathways related to starch and sucrose metabolism, galactose metabolism, and amino acid biosynthesis, which play critical roles in drought adaptation.</p><p><strong>Conclusion: </strong>These findings provide insights into how PGPR contributes to stress resilience in <i>B. juncea</i> by modulating key biochemical pathways. This study provides new molecular insights into the known effect of PGPR for mitigating drought stress in oilseed crops.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 6","pages":""},"PeriodicalIF":3.4000,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195340/pdf/","citationCount":"0","resultStr":"{\"title\":\"Metabolomic Profiling Reveals PGPR-Driven Drought Tolerance in Contrasting <i>Brassica juncea</i> Genotypes.\",\"authors\":\"Asha Rani Sheoran, Nita Lakra, Baljeet Singh Saharan, Annu Luhach, Yogesh K Ahlawat, Rosa Porcel, Jose M Mulet, Prabhakar Singh\",\"doi\":\"10.3390/metabo15060416\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Drought stress is a major abiotic factor limiting <i>Brassica juncea</i> productivity, resulting in significant yield reductions. Plant Growth-Promoting Rhizobacteria (PGPR) have shown potential in enhancing drought tolerance; however, the metabolomic changes associated with their effects remain largely unexplored. This study examines the metabolic changes induced by a PGPR consortium (<i>Enterobacter hormaechei</i>, <i>Pantoea dispersa</i>, and <i>Acinetobacter</i> sp.) in two contrasting genotypes <i>B. juncea</i> (L.) Czern. 'RH 725' (drought tolerant) and <i>B. juncea</i> (L.) Czern. 'RH-749' (drought sensitive for drought tolerance, under both control and drought conditions.</p><p><strong>Methods: </strong>Metabolite profiling was conducted using gas chromatography-mass spectrometry (GC-MS) to identify compounds that accumulated differentially across treatments. We applied multivariate statistical methods, such as Partial Least Squares Discriminant Analysis (PLS-DA), hierarchical clustering, and pathway enrichment analysis, to explore metabolic reprogramming.</p><p><strong>Results: </strong>Drought stress induced significant changes in metabolite profile, particularly increasing the levels of osmoprotectants such as trehalose, glucose, sucrose, proline, and valine. Additionally, alterations in organic acids (malic acid and citric acid) and fatty acids (oleic acid and linoleic acid) were observed. PGPR inoculation further amplified these metabolic responses to enhance the osmotic regulation, reactive oxygen species (ROS) detoxification, and carbon-nitrogen metabolism, with RH-725 displaying a stronger adaptive response. Pathway enrichment analysis revealed that PGPR treatment significantly influenced metabolic pathways related to starch and sucrose metabolism, galactose metabolism, and amino acid biosynthesis, which play critical roles in drought adaptation.</p><p><strong>Conclusion: </strong>These findings provide insights into how PGPR contributes to stress resilience in <i>B. juncea</i> by modulating key biochemical pathways. This study provides new molecular insights into the known effect of PGPR for mitigating drought stress in oilseed crops.</p>\",\"PeriodicalId\":18496,\"journal\":{\"name\":\"Metabolites\",\"volume\":\"15 6\",\"pages\":\"\"},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2025-06-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195340/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Metabolites\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.3390/metabo15060416\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Metabolites","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/metabo15060416","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Background: Drought stress is a major abiotic factor limiting Brassica juncea productivity, resulting in significant yield reductions. Plant Growth-Promoting Rhizobacteria (PGPR) have shown potential in enhancing drought tolerance; however, the metabolomic changes associated with their effects remain largely unexplored. This study examines the metabolic changes induced by a PGPR consortium (Enterobacter hormaechei, Pantoea dispersa, and Acinetobacter sp.) in two contrasting genotypes B. juncea (L.) Czern. 'RH 725' (drought tolerant) and B. juncea (L.) Czern. 'RH-749' (drought sensitive for drought tolerance, under both control and drought conditions.
Methods: Metabolite profiling was conducted using gas chromatography-mass spectrometry (GC-MS) to identify compounds that accumulated differentially across treatments. We applied multivariate statistical methods, such as Partial Least Squares Discriminant Analysis (PLS-DA), hierarchical clustering, and pathway enrichment analysis, to explore metabolic reprogramming.
Results: Drought stress induced significant changes in metabolite profile, particularly increasing the levels of osmoprotectants such as trehalose, glucose, sucrose, proline, and valine. Additionally, alterations in organic acids (malic acid and citric acid) and fatty acids (oleic acid and linoleic acid) were observed. PGPR inoculation further amplified these metabolic responses to enhance the osmotic regulation, reactive oxygen species (ROS) detoxification, and carbon-nitrogen metabolism, with RH-725 displaying a stronger adaptive response. Pathway enrichment analysis revealed that PGPR treatment significantly influenced metabolic pathways related to starch and sucrose metabolism, galactose metabolism, and amino acid biosynthesis, which play critical roles in drought adaptation.
Conclusion: These findings provide insights into how PGPR contributes to stress resilience in B. juncea by modulating key biochemical pathways. This study provides new molecular insights into the known effect of PGPR for mitigating drought stress in oilseed crops.
MetabolitesBiochemistry, Genetics and Molecular Biology-Molecular Biology
CiteScore
5.70
自引率
7.30%
发文量
1070
审稿时长
17.17 days
期刊介绍:
Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.