妊娠期糖尿病和健康妊娠中免疫细胞亚群比率(NKT/NK, Th/Tc, B1/B2)的比较评估:与生化和免疫化学谱的联系

IF 3.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Metabolites Pub Date : 2025-06-08 DOI:10.3390/metabo15060378
Jelena Omazić, Andrijana Muller, Mirta Kadivnik, Blaženka Dobrošević, Barbara Vuković, Mirela Florijančić, Jasenka Wagner
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引用次数: 0

摘要

妊娠期糖尿病(GD)是一种常见的妊娠代谢紊乱,涉及免疫改变。虽然免疫细胞与妊娠期妊娠之间存在联系,但NKT/NK、辅助性/细胞毒性T和B1/B2淋巴细胞比率在伴有妊娠期妊娠和不伴有妊娠期妊娠的复杂/非复杂妊娠中的具体作用尚不清楚。这项横断面研究假设,这些淋巴细胞比例的特定失衡将出现在妊娠期,这些比例将与妊娠期的关键代谢参数相关。方法:通过流式细胞术和生化指标的测定,我们比较了162名妊娠晚期妇女的淋巴细胞比率,分为四组:健康无并发症(n = 40)、健康并发症(n = 40)、妊娠无并发症(n = 42)和妊娠合并妊娠(n = 40)。结果:妊娠期与正常妊娠间淋巴细胞比值无显著差异。新的相关性出现了:在整个队列中,NKT/NK比值与c肽和甘油三酯呈正相关,与HDL胆固醇呈负相关。辅助/细胞毒比与胰岛素和c肽呈负相关。GD组NKT/NK与c肽呈正相关,辅助性/细胞毒性与胰岛素呈负相关。结论:这些发现提示免疫细胞亚群与妊娠和妊娠期代谢状态之间存在微妙但重要的联系,值得进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative Assessment of Immune Cell Subset Ratios (NKT/NK, Th/Tc, B1/B2) in Gestational Diabetes and Healthy Pregnancy: Links to Biochemical and Immunochemical Profiles.

Introduction: Gestational diabetes (GD) is a common pregnancy metabolic disorder involving immune alterations. While there is a link between immune cells and GD, the specific roles of NKT/NK, helper/cytotoxic T, and B1/B2 lymphocyte ratios in complicated/uncomplicated pregnancies with and without GD are underexplored. This cross-sectional study hypothesized that specific imbalances in these lymphocyte ratios would be present in GD, and that these ratios would correlate with key metabolic parameters in pregnancy. Methods: We compared these lymphocyte ratios in 162 third-trimester pregnant women across four groups: healthy uncomplicated (n = 40), healthy complicated (n = 40), GD uncomplicated (n = 42), and GD complicated (n = 40), using flow cytometry and by measuring biochemical parameters. Results: No significant differences in lymphocyte ratios were found between GD and healthy pregnancies. Novel correlations emerged: in the entire cohort, the NKT/NK ratio positively correlated with C-peptide and triglycerides, and negatively with HDL cholesterol. The helper/cytotoxic ratio negatively correlated with insulin and C-peptide. In the GD group, NKT/NK correlated positively with C-peptide, and helper/cytotoxic negatively with insulin. Conclusion: These findings suggest a subtle yet significant link between immune cell subsets and metabolic status in pregnancy and GD, warranting further investigation.

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来源期刊
Metabolites
Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology
CiteScore
5.70
自引率
7.30%
发文量
1070
审稿时长
17.17 days
期刊介绍: Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.
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