健康纯种母马围产期血浆代谢组学分析。

IF 2.4 2区 农林科学 Q1 VETERINARY SCIENCES
Junjie Li, Touko Matsumoto, Hong Liu, Chunmei Li, Harutaka Murase, Yuki Yamamoto, Kentaro Nagaoka
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引用次数: 0

摘要

背景:准确预测分娩时间对确保母马和马驹的健康和福祉至关重要。然而,马怀孕的特点是妊娠期长短、独特的内分泌机制和分娩前微妙的生理变化。目的:应用代谢组学方法研究母马分娩前后外周血代谢物的特征性变化。研究设计:纵向体内代谢研究。方法:选取成功分娩的纯种母马(n = 9),于分娩前4天至分娩后7天采集血浆样本95份,采用气相色谱-质谱法进行非靶向代谢组学分析。采用主成分分析(PCA)和层次聚类分析(hierarchical clustering analysis)对不同组进行比较。采用重复测量方差分析(RMANOVA)鉴定各种代谢物。富集分析寻找相关的代谢组学途径。结果:主成分分析和层次聚类分析显示出产前、分娩和分娩后的聚类分离。RMANOVA显示62种代谢物在所有时间点上存在显著差异(错误发现率)主要局限性:本研究中纳入的动物和样本数量有限,并且未考虑母马的生殖史。此外,本研究未进行定量研究,以确定关键代谢物的具体浓度和范围。结论:G3P是一种潜在的预测分娩的生物标志物。本研究为母马围产期血液代谢变化提供了新的认识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Plasma metabolomic profiling during peri-parturition in healthy Thoroughbred mares.

Background: Accurate prediction of the timing of parturition is crucial to ensure the health and well-being of both mares and foals. However, equine pregnancies are characterised by significant variability in gestation length, unique endocrine mechanisms, and subtle physiological changes before parturition.

Objectives: To investigate the characteristic changes in the peripheral metabolites of mares before and after parturition using metabolomic approaches.

Study design: Longitudinal in vivo metabolic study.

Methods: Plasma samples (n = 95) were collected from successfully foaling Thoroughbred mares (n = 9) from 4 days before to 7 days after parturition, and a non-targeted metabolomic analysis was performed using GC-MS. PCA and hierarchical clustering analysis were used to compare different groups. Repeated measures ANOVA (RMANOVA) was employed to identify the various metabolites. Enrichment analysis was performed to find the related metabolomic pathways.

Results: PCA and hierarchical clustering analysis demonstrated cluster separation between pre-parturition, parturition, and post-parturition. RMANOVA revealed significant differences in 62 metabolites across all time points (False Discovery Rate <0.05). These metabolites were significantly enriched in multiple metabolic pathways, including valine, leucine, and isoleucine biosynthesis; galactose metabolism; arginine biosynthesis; valine, leucine, and isoleucine degradation; alanine, aspartate, and glutamate metabolism; glyoxylate and dicarboxylate metabolism; and pantothenate and CoA biosynthesis. Among these metabolites, glycerol-3-phosphate (G3P) showed interesting changes that increased 3 days before parturition.

Main limitations: The number of animals and samples included in this study was limited, and the reproductive history of the mares was not considered. In addition, this study did not conduct quantitative research to determine the specific concentrations and ranges of the key metabolites.

Conclusions: G3P is a potential biomarker for predicting parturition. This research provides new insights into mares' periparturition blood metabolic changes.

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来源期刊
Equine Veterinary Journal
Equine Veterinary Journal 农林科学-兽医学
CiteScore
5.10
自引率
13.60%
发文量
161
审稿时长
6-16 weeks
期刊介绍: Equine Veterinary Journal publishes evidence to improve clinical practice or expand scientific knowledge underpinning equine veterinary medicine. This unrivalled international scientific journal is published 6 times per year, containing peer-reviewed articles with original and potentially important findings. Contributions are received from sources worldwide.
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