认知衰退老年大鼠海马转录组测序及生物信息学分析

IF 2.6 3区心理学 Q2 BEHAVIORAL SCIENCES

Behavioural Brain Research Pub Date : 2025-06-24 DOI:10.1016/j.bbr.2025.115711

Li Hou , Ling Xin , Yuru Liu , Bin He , Cuige Shi , Yishu Yang

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引用次数: 0

摘要

与年龄相关的认知能力下降对健康老龄化提出了重大挑战，但其潜在的分子机制仍然知之甚少。在这项研究中，我们采用Morris水迷宫和海马转录组分析来研究大鼠模型中与年龄相关的认知能力下降。与年轻大鼠（RY）相比，老年大鼠（RA）表现出明显的空间记忆缺陷。转录组分析发现，与RY组相比，RA组海马中有21个差异表达基因（DEGs），其中54个基因上调，67个基因下调。qRT-PCR验证显示，与RY组相比，RA组Cd74和Cd4表达显著上调，Col1a1、Col3a1和Serpine1表达显著下调。生物信息学分析显示，这些deg在慢性炎症、蛋白质平衡丧失和细胞外基质途径的生物过程中富集。这些发现表明，海马转录组改变可能有助于认知衰老，为认知功能提供潜在的预测因子，并为探索分子机制奠定基础。

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Transcriptome sequencing and bioinformatics analysis of hippocampus in aged rats with cognitive decline

Age-related cognitive decline poses significant challenges to healthy aging, yet its underlying molecular mechanisms remain poorly understood. In this study, we employed Morris Water Maze and hippocampal transcriptome analysis to investigate age-related cognitive decline in a rat model. Aged rats (RA) exhibited significant spatial memory deficits compared to young rats (RY). Transcriptome analysis identified ‌121 differentially expressed genes (DEGs)‌ in the hippocampus ‌of‌ RA group compared with RY group, including ‌54 up-regulated and 67 down-regulated genes. The qRT-PCR validation revealed significant up-regulation of Cd74 and Cd4 expression, along with marked down-regulation of Col1a1, Col3a1, and Serpine1 expression in RA group compared to RY group. Bioinformatics analysis revealed these DEGs were enriched in the biological processes of chronic inflammation, loss of proteostasis, and extracellular matrix pathways. These findings suggest hippocampal transcriptomic alterations may contribute to cognitive aging, providing potential predictors for cognitive function and ‌a‌ foundation for exploring molecular mechanisms.

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来源期刊

Behavioural Brain Research 医学-行为科学

CiteScore

5.60

自引率

0.00%

发文量

383

审稿时长

61 days

期刊介绍： Behavioural Brain Research is an international, interdisciplinary journal dedicated to the publication of articles in the field of behavioural neuroscience, broadly defined. Contributions from the entire range of disciplines that comprise the neurosciences, behavioural sciences or cognitive sciences are appropriate, as long as the goal is to delineate the neural mechanisms underlying behaviour. Thus, studies may range from neurophysiological, neuroanatomical, neurochemical or neuropharmacological analysis of brain-behaviour relations, including the use of molecular genetic or behavioural genetic approaches, to studies that involve the use of brain imaging techniques, to neuroethological studies. Reports of original research, of major methodological advances, or of novel conceptual approaches are all encouraged. The journal will also consider critical reviews on selected topics.