在非洲家猫中发现的首个分子证据及其在整个非洲大陆的分布概况

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2025-06-24 DOI:10.1016/j.vprsr.2025.101302

Ioana Bianca Mitrea , Petr Cibulka , Angela Monica Ionică , Nonzuzo Audrey Ndlovu , Norman Leo Mukarati , Thokozani Hove , Noureddine Mechouk , Mihai Sorin Cernea , Andrei Daniel Mihalca

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引用次数: 0

摘要

家猫（Felis catus）可感染多种心肺线虫。虽然细纹线虫在全球广泛分布，但来自非洲的报道很少，而且主要是野外报道。此外，其中一些报告被认为是误认。非洲唯一一份关于家猫的报告是20多年前的，起源于肯尼亚。本研究的目的是利用光镜和分子鉴定方法对津巴布韦哈拉雷家猫中存在的抽象猫进行形态学评价。本研究共选取了57只家猫，采用Baermann技术对粪便样本进行了分析。收集检测到的幼虫，对其进行形态鉴定，并针对3个基因进行分子分析。57份样品中有1份为第一阶段幼虫（L1）阳性，根据形态特征鉴定为细圆线虫（aeluro圆线虫）。分子分析随后证实了该物种的身份。本研究首次在非洲家猫和南部非洲家猫中发现了深螺线虫的分子证实。建议今后进一步研究其分布和流行病学。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

First molecular evidence of Aelurostrongylus abstrusus in domestic cats from Africa and a synopsis of its occurrence across the continent

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First molecular evidence of Aelurostrongylus abstrusus in domestic cats from Africa and a synopsis of its occurrence across the continent

Domestic cats (Felis catus) can be infected with a variety of cardio-pulmonary nematodes. Although Aelurostrongylus abstrusus is widely distributed globally, reports from Africa are scarce and refer mainly to wild felids. Moreover, some of these reports are considered as misidentifications. The only report in domestic cats in Africa is more than 20 years old and originated from Kenya. The aim of this study was to evaluate the presence of A. abstrusus in domestic cats from Harare, Zimbabwe morphologically on light microscopy and confirmation by molecular means.

A total of 57 domestic cats were included in this study, and fecal samples were analyzed using the Baermann technique. The detected larvae were collected, morphologically identified, and subsequently confirmed through molecular analysis by targeting three genes.

One sample out of 57 tested positive for first-stage larvae (L1) and was identified as Aelurostrongylus abstrusus based on morphological characteristics. Molecular analysis subsequently confirmed the species identity.

The present study is the first molecular confirmation of Aelurostrongylus abstrusus in domestic cats from Africa and the first case in domestic cats from southern Africa. Future studies are recommended to further investigate its distribution and epidemiology.

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).