多发性硬化症患者的神经束蛋白155、186和140抗体:一项前瞻性先导探索性研究

IF 0.9 3区 医学 Q4 NEUROSCIENCES
Neurology India Pub Date : 2025-05-01 Epub Date: 2025-05-23 DOI:10.4103/neurol-india.Neurol-India-D-25-00046
Thomas Mathew, Surabhi Garg, Pritam Majumdar, Shagun Bhardwaj, Sudheeran Kannoth, Annamma Mathai, Sumithra Selvam, Molly George, Uday Murgod, Vikram Kamath, Subhash Kaul, Sindhu V Nambiar, Akshata Huddar, Sonia Shivde, Sagar Badachi, Raghunandan Nadig, Sharath Gg Kumar, Sai Kanth Deepalam, Grk Sarma
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引用次数: 0

摘要

背景:在中枢和外周脱髓鞘疾病中发现了抗神经束蛋白抗体(ANFA)。我们介绍了在印度多发性硬化症(MS)患者队列中针对神经束蛋白(NF) 155、186和140的pan-ANFA检测结果。方法:在这项多中心前瞻性研究中,我们调查了满足麦当劳2017标准的印度MS患者中针对NF155、NF186和NF140的抗体的存在。ANFA测定采用竞争ELISA技术,使用人神经束素。结果:在2023年11月1日至2024年9月30日的11个月期间,我们对71例MS患者进行了ANFA检测。在71例MS患者中,57例(80.28%)至少检测出一种抗神经束蛋白抗体阳性,15例(21.13%)检测出两种抗体阳性。分离ANFA140 26例(36.62%),分离ANFA186 13例(18.31%),分离ANFA155 3例(4.23%)。联合ANFA 186/140患者14例(19.72%),联合ANFA 155/140患者1例(1.41%)。结论:在相当数量的印度MS患者中存在至少一种神经束蛋白抗原的抗体。与对NF155的ANFA相比,对NF140和NF186的ANFA更为普遍。需要进一步的免疫学和分子研究来确认这些抗体是真正的致病性、疾病修饰性还是偶然的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Neurofascin 155, 186 and 140 Antibodies in Patients with Multiple Sclerosis: A Pilot Prospective Exploratory Study.

Background: Antineurofascin antibodies (ANFA) have been identified in central and peripheral demyelinating disorders. We present the results of pan-ANFA assay targeting neurofascin (NF) 155, 186, and 140, in a cohort of Indian Multiple Sclerosis (MS) patients.

Objective: To assess the presence of antineurofascin antibodies (ANFA) 155, 186, and 140 in patients with MS.

Methodology: In this multicenter prospective study, we investigated the presence of antibodies targeting NF155, NF186, and NF140 in Indian MS patients satisfying McDonald's 2017 criteria. ANFA was measured by competitive ELISA technique using human neurofascins.

Results: During the 11-month period from November 1, 2023, to September 30, 2024, we tested 71 MS patients for ANFA. Of the 71 MS patients, 57 (80.28%) tested positive for at least one antineurofascin antibody and 15 (21.13%) were positive for two antibodies. Isolated ANFA140 was present in 26 (36.62%) patients, isolated ANFA186 in 13 (18.31%), and isolated ANFA155 in 3 (4.23%). Combined ANFA 186/140 was present in 14 (19.72%) patients and combined ANFA 155/140 in 1 (1.41%).

Conclusion: Antibodies to at least one of the neurofascin antigens were present in a significant number of Indian MS patients. ANFA to NF140 and NF186 was found to be more prevalent compared with ANFA against NF155. Further immunological and molecular investigations are required to confirm whether these antibodies are truly pathogenic, disease-modifying, or incidental.

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来源期刊
Neurology India
Neurology India 医学-神经科学
CiteScore
1.60
自引率
70.40%
发文量
434
审稿时长
2 months
期刊介绍: Neurology India (ISSN 0028-3886) is Bi-monthly publication of Neurological Society of India. Neurology India, the show window of the progress of Neurological Sciences in India, has successfully completed 50 years of publication in the year 2002. ‘Neurology India’, along with the Neurological Society of India, has grown stronger with the passing of every year. The full articles of the journal are now available on internet with more than 20000 visitors in a month and the journal is indexed in MEDLINE and Index Medicus, Current Contents, Neuroscience Citation Index and EMBASE in addition to 10 other indexing avenues. This specialty journal reaches to about 2000 neurologists, neurosurgeons, neuro-psychiatrists, and others working in the fields of neurology.
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