巴拉圭猫血浆的存在、遗传特征、地理分布和相关危险因素。

IF 1.9 4区 生物学 Q3 MICROBIOLOGY
Brazilian Journal of Microbiology Pub Date : 2025-09-01 Epub Date: 2025-06-23 DOI:10.1007/s42770-025-01714-w
María Fátima Rodriguez Valinotti, Lucía Soledad Patiño, Rodrigo Mereles Achón, Magaly Martinez, Antonio Rodriguez, Rosmary Rodriguez Valinotti, Amaias Ávalos
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引用次数: 0

摘要

嗜血性支原体是一种小的、无壁的细菌,寄生在红细胞上,可引起猫的溶血性贫血。全世界已知感染猫的三种主要物种是血支原体(Mhf)、血支原体(CMht)和结核支原体(CMt)。这些物种的致病性和流行率不同,Mhf致病性最强,CMht最普遍。本研究的目的是确定巴拉圭猫血浆的存在、遗传特征、相关危险因素和地理分布。从当地兽医提交给CEDIVEP实验室的猫全血样本中提取DNA,用于检测猫的血浆;通过聚合酶链反应(PCR)检测Mhf(456份)、CMht(428份)和CMt(359份)。Mhf阳性76/456只(16%),CMht阳性77/428只(18%),没有动物CMt PCR阳性。测序、BLAST和系统发育分析证实了16s rRNA的身份,并得到了物种特异性进化枝明显分离的支持。在该国的两个地区(东部和西部)均发现了阳性动物,发病率最高的省是中部,70/76 (92.1%)Mhf阳性,70/77 (90.9%)CMht阳性。采用聚合酶链反应测定巴拉圭两个地区家猫中猫血浆的流行情况。男性是Mhf和CMht的危险因素。年龄在1至3岁之间是CMht和混合品种的危险因素,暹罗是Mhf的危险因素。猫支原体病在巴拉圭中部省存在较多,并且更常影响杂交猫和普通欧洲猫。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Presence, genetic characterization, geographic distribution and associated risk factors of feline hemoplasmas in Paraguay.

Hemotropic mycoplasmas (hemoplasmas) are small, wall-less bacteria that parasitize red blood cells and can induce hemolytic anemia in felines. The three main species known to infect cats worldwide are Mycoplasma haemofelis (Mhf), Candidatus Mycoplasma haemominutum (CMht) and Candidatus Mycoplasma turicencis (CMt). These species differ in their pathogenicity and prevalence, Mhf being the most pathogenic and CMht the most prevalent. The aim of this study was to determine the presence, genetic characterization, associated risk factors and geographical distribution of feline hemoplasmas in Paraguay. DNA was extracted from feline whole blood samples submitted by local veterinarians to the CEDIVEP laboratory for the detection of feline hemoplasmas; Mhf (456 samples), CMht (428 samples), and CMt (359 samples) by polymerase chain reaction (PCR). A total of 76/456 samples (16%) were positive for Mhf, 77/428 (18%) were positive for CMht, and no animals were positive for CMt by PCR. Sequencing, BLAST and phylogenetic analysis were performed to confirm the identity of 16 S rRNA and was supported by the distinct separation of species-specific clades. Positive animals were found in both regions of the country (eastern and western), and the Department with the highest prevalence was Central with 70/76 (92,1%) positive for Mhf and 70/77 (90,9%) positive for CMht. The prevalence of feline hemoplasmas in domestic cats in both regions of Paraguay was determined by PCR. Male sex was a risk factor for Mhf and CMht. Age between 1 and 3 years was a risk factor for CMht and mixed breed and Siamese was a risk factor for Mhf. Feline mycoplasmosis had a greater presence in Central deparment Paraguay and more frequently affected mixed breed and common European cats.

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来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
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