Ghazwa Boudali, Insaf Djebbi, Wided Ben Ammar, Chiraz Cheffei-Haouari
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The addition of Zincum Metallicum: an homeopathic during germination and its effect against zinc-induced metal stress.
In the present study, we set out to investigate the effect of Zincum Metallicum (ZM) supply on zinc (Zn) toxicity, using Lepidium sativum germination, zinc up take, and numerous biochemical parameters. The experiment was conducted in a growth chamber with seedlings exposed to 0, 500, 1000, 1500, and 2000 µM Zn for 7 days in the absence or presence of 15CH or 9CH ZM. L. sativum plant hypocotyl and radicles produced less fresh biomass when Zn was present, and this impact was most pronounced at the highest Zn concentration (2000 M). Zinc stress also induced lipid peroxidation, according to the amplified levels of malondialdehyde (MDA) and an important increase in the proline levels both in hypocotyl and radicles. Under Zn-toxicity (500, 1000, and 1500 µM), speed and water content were decreased and/or unaltered, while at 2000 µM Zn, the effect of zinc toxicity was very pronounced. Additionally, the supply of ZM in the nutrient medium considerably ameliorated the seeds speed germination, water content, MDA, and proline production. Our data suggest that the potential homeopathy properties of ZM may be efficiently involved in the restriction of Zn induced damages, by lowering Zn accumulation and translocation in L. sativum.
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