{"title":"二甲双胍对污水污泥厌氧消化的抑制作用:关注微生物代谢物和群落/功能基因的变化","authors":"Jishi Zhang , Ruixi Wang , Yu Gu , Zhaoyi Duan , Zhengyi Zhang","doi":"10.1016/j.jwpe.2025.108198","DOIUrl":null,"url":null,"abstract":"<div><div>Metformin (MEF) is commonly used in the treatment of diabetes and is often detected in sewage sludge, but its effect on anaerobic digestion (AD) for methane (CH<sub>4</sub>) production has rarely been investigated. This study revealed the influence of MEF on AD of sewage sludge. MEF significantly interfered with AD process, causing CH<sub>4</sub> yield reduction of 9.67 % and 30.18 % exposure on 6 mM and 9 mM of MEF, compared to the control groups without MEF, respectively. Liquid phase analyses revealed that MEF led to the accumulation of butyric and acetic acids and the increase in protein and polysaccharide contents at the initial stage of AD, showing the sludge dissolution. In addition, the high-throughput sequencing identified <em>Firmicutes</em> as the main contributor to the accumulation of volatile fatty acids (VFAs), with its relative abundance rising from 35.45 % to 47.48 %. Conversely, the relative abundance of <em>Methanosaeta</em> decreased from 56.21 % to 36.0 %, causing low CH<sub>4</sub> yield. Furthermore, an analysis of the changes in enzyme abundance involved in metabolic processes indicated that MEF could disrupt the metabolic pathway, thereby inhibiting CH<sub>4</sub> yield. Surprisingly, MEF could not be decomposed by anaerobes while it could be adsorbed by the sludge in AD. Thus, low cost and efficient pretreatment techniques for the PPCPs containing sewage waste are recommended to obtain suitable substrates for more CH<sub>4</sub>.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"76 ","pages":"Article 108198"},"PeriodicalIF":6.3000,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Inhibition of metformin on sewage sludge anaerobic digestion: Focusing on changes in microbial metabolites and community/functional genes\",\"authors\":\"Jishi Zhang , Ruixi Wang , Yu Gu , Zhaoyi Duan , Zhengyi Zhang\",\"doi\":\"10.1016/j.jwpe.2025.108198\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Metformin (MEF) is commonly used in the treatment of diabetes and is often detected in sewage sludge, but its effect on anaerobic digestion (AD) for methane (CH<sub>4</sub>) production has rarely been investigated. This study revealed the influence of MEF on AD of sewage sludge. MEF significantly interfered with AD process, causing CH<sub>4</sub> yield reduction of 9.67 % and 30.18 % exposure on 6 mM and 9 mM of MEF, compared to the control groups without MEF, respectively. Liquid phase analyses revealed that MEF led to the accumulation of butyric and acetic acids and the increase in protein and polysaccharide contents at the initial stage of AD, showing the sludge dissolution. In addition, the high-throughput sequencing identified <em>Firmicutes</em> as the main contributor to the accumulation of volatile fatty acids (VFAs), with its relative abundance rising from 35.45 % to 47.48 %. Conversely, the relative abundance of <em>Methanosaeta</em> decreased from 56.21 % to 36.0 %, causing low CH<sub>4</sub> yield. Furthermore, an analysis of the changes in enzyme abundance involved in metabolic processes indicated that MEF could disrupt the metabolic pathway, thereby inhibiting CH<sub>4</sub> yield. Surprisingly, MEF could not be decomposed by anaerobes while it could be adsorbed by the sludge in AD. Thus, low cost and efficient pretreatment techniques for the PPCPs containing sewage waste are recommended to obtain suitable substrates for more CH<sub>4</sub>.</div></div>\",\"PeriodicalId\":17528,\"journal\":{\"name\":\"Journal of water process engineering\",\"volume\":\"76 \",\"pages\":\"Article 108198\"},\"PeriodicalIF\":6.3000,\"publicationDate\":\"2025-06-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of water process engineering\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S221471442501270X\",\"RegionNum\":2,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, CHEMICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of water process engineering","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S221471442501270X","RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, CHEMICAL","Score":null,"Total":0}
Inhibition of metformin on sewage sludge anaerobic digestion: Focusing on changes in microbial metabolites and community/functional genes
Metformin (MEF) is commonly used in the treatment of diabetes and is often detected in sewage sludge, but its effect on anaerobic digestion (AD) for methane (CH4) production has rarely been investigated. This study revealed the influence of MEF on AD of sewage sludge. MEF significantly interfered with AD process, causing CH4 yield reduction of 9.67 % and 30.18 % exposure on 6 mM and 9 mM of MEF, compared to the control groups without MEF, respectively. Liquid phase analyses revealed that MEF led to the accumulation of butyric and acetic acids and the increase in protein and polysaccharide contents at the initial stage of AD, showing the sludge dissolution. In addition, the high-throughput sequencing identified Firmicutes as the main contributor to the accumulation of volatile fatty acids (VFAs), with its relative abundance rising from 35.45 % to 47.48 %. Conversely, the relative abundance of Methanosaeta decreased from 56.21 % to 36.0 %, causing low CH4 yield. Furthermore, an analysis of the changes in enzyme abundance involved in metabolic processes indicated that MEF could disrupt the metabolic pathway, thereby inhibiting CH4 yield. Surprisingly, MEF could not be decomposed by anaerobes while it could be adsorbed by the sludge in AD. Thus, low cost and efficient pretreatment techniques for the PPCPs containing sewage waste are recommended to obtain suitable substrates for more CH4.
期刊介绍:
The Journal of Water Process Engineering aims to publish refereed, high-quality research papers with significant novelty and impact in all areas of the engineering of water and wastewater processing . Papers on advanced and novel treatment processes and technologies are particularly welcome. The Journal considers papers in areas such as nanotechnology and biotechnology applications in water, novel oxidation and separation processes, membrane processes (except those for desalination) , catalytic processes for the removal of water contaminants, sustainable processes, water reuse and recycling, water use and wastewater minimization, integrated/hybrid technology, process modeling of water treatment and novel treatment processes. Submissions on the subject of adsorbents, including standard measurements of adsorption kinetics and equilibrium will only be considered if there is a genuine case for novelty and contribution, for example highly novel, sustainable adsorbents and their use: papers on activated carbon-type materials derived from natural matter, or surfactant-modified clays and related minerals, would not fulfil this criterion. The Journal particularly welcomes contributions involving environmentally, economically and socially sustainable technology for water treatment, including those which are energy-efficient, with minimal or no chemical consumption, and capable of water recycling and reuse that minimizes the direct disposal of wastewater to the aquatic environment. Papers that describe novel ideas for solving issues related to water quality and availability are also welcome, as are those that show the transfer of techniques from other disciplines. The Journal will consider papers dealing with processes for various water matrices including drinking water (except desalination), domestic, urban and industrial wastewaters, in addition to their residues. It is expected that the journal will be of particular relevance to chemical and process engineers working in the field. The Journal welcomes Full Text papers, Short Communications, State-of-the-Art Reviews and Letters to Editors and Case Studies