缺氧相关的miRNA在子痫前期的失调：miR-210和miR-383的作用

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-06-14 DOI:10.1016/j.genrep.2025.102278

Nilgun Cekin , Seyda Akin , Irem Kucukyildiz , Ergun Pinarbasi

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引用次数: 0

摘要

胎盘血流灌注不足被认为是子痫前期（PE）的一个促进因素，导致胎盘持续缺氧。在缺氧条件下，某些microrna （mirna）表现出不同的表达水平。文献表明，miR-210是缺氧反应途径的组成部分，包括血管生成、线粒体呼吸、细胞存活和DNA修复。相反，miR-383被认为通过靶向血管内皮生长因子和乳酸脱氢酶a等基因来调节缺氧诱导因子1- α (HIF-1α)的基本作用，这些基因在内皮细胞中受HIF-1α调节。本研究旨在评估miR-210和miR-383在缺氧条件下的表达水平，使用定量聚合酶链反应（qPCR）在子痫前期（PE）妇女（N = 22）和对照组（N = 15）的母体血液（MB）、胎盘组织（PT）和脐带血（CB）样本中评估miR-210和miR-383表达水平。研究结果表明，与对照组相比，PE组所有样本中miR-210的表达增加，而miR-383的表达减少。在PE亚组中，重度PE组和轻度PE组中，MB组miR-210水平分别增加了3.78倍和2.73倍，PT组分别增加了3.67倍和2.49倍，CB组分别增加了5.48倍和5.04倍(p <；0.01)。相比之下，仅在PT中观察到miR-383表达显著降低，严重PE组miR-383表达降低2.22倍（p = 0.01），轻度PE组miR-383表达降低7.69倍（p = 0.002）。重度PE组与轻度PE组比较，miR-210在MB和PT中的表达以及miR-383在PT中的表达差异有统计学意义(p = 0.01, p <；0.001, p = 0.001)。方差分析显示，miR-210在所有样本中均存在显著差异，而miR-383仅在PT中具有显著性(p <；0.001)。这些发现表明，miR-210在缺氧条件下的PE病理生理中起关键作用，而miR-383的降低，特别是在轻度PE中，可能有助于病情的进展。

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Hypoxia-related miRNA dysregulation in preeclampsia: The roles of miR-210 and miR-383

Insufficient placental blood perfusion is recognized as a contributing factor to preeclampsia (PE), resulting in persistent placental hypoxia. Under hypoxic conditions, certain microRNAs (miRNAs) exhibit varying expression levels. The literature suggests that miR-210 is integral to hypoxia response pathways, including angiogenesis, mitochondrial respiration, cell survival, and DNA repair. Conversely, miR-383 is believed to modulate the fundamental effects of hypoxia-inducible factor 1-alpha (HIF-1α) by targeting genes such as vascular endothelial growth factor and lactate dehydrogenase-A, which are regulated by HIF-1α in endothelial cells. This study aimed to evaluate miR-210 and miR-383 expression levels, anticipated to change under hypoxic conditions, using quantitative polymerase chain reaction (qPCR) in maternal blood (MB), placental tissue (PT), and umbilical cord blood (CB) samples from women with preeclampsia (PE) (N = 22) and control (N = 15). The study findings indicate that the expression of miR-210 increased, while the expression of miR-383 decreased in all samples from the PE group compared to the control group. In PE subgroups, miR-210 levels increased in MB by 3.78-fold and 2.73-fold, in PT by 3.67-fold and 2.49-fold, and in CB by 5.48-fold and 5.04-fold in the severe and mild PE groups, respectively (p < 0.01). In contrast, a significant decrease in miR-383 expression was observed only in PT, with a 2.22-fold reduction (p = 0.01) in the severe PE group and a 7.69-fold reduction (p = 0.002) in the mild PE group. Evaluations comparing the severe and mild PE groups revealed significant differences in the expression of miR-210 in MB and PT, as well as miR-383 in PT (p = 0.01, p < 0.001, and p = 0.001, respectively). According to the ANOVA test, miR-210 showed significant differences across all samples, while miR-383 showed significance only in PT (p < 0.001). These findings suggest that miR-210 plays a key role in PE pathophysiology under hypoxic conditions, while miR-383 reduction, particularly in mild PE, may contribute to the condition's progression.

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.