{"title":"基于智能手机的化学发光平台集成微流控柔性试纸条用于片上微混合和葡萄糖传感","authors":"Kavita Manekar;Meghana A. Hasamnis","doi":"10.1109/LSENS.2025.3572024","DOIUrl":null,"url":null,"abstract":"Efficient micromixing and accurate sensing are essential for advancing lab-on-a-chip platforms in biomedical applications. This work introduces a novel microfluidic flexible test strip for on-chip glucose sensing, leveraging a serpentine-shaped microchannel optimized through design and simulation in a software tool to enhance passive mixing of two different fluid samples. The integrated system utilizes a smartphone-based chemiluminescence (CL) detection approach for real-time glucose quantification. A 3D-printed device ensures precise camera alignment for optimal luminescence capture. The polydimethylsiloxane-based microfluidic test strip enables rapid and cost-effective glucose detection, serving as a portable alternative to conventional sensors. Experimental validation demonstrates 97.47% mixing efficiency, ensuring uniform reaction conditions. The system exhibits a strong linear response between 3 and 7 mM, with a 0.3-mM detection limit. CL intensity correlates positively with glucose concentration, following the linear regression, while expected vs. estimated glucose values yield an <italic>R</i><sup>2</sup> of 0.94, confirming high accuracy. These findings highlight the potential of microfluidic-assisted flexible test strips for point-of-care diagnostics, paving the way for advanced biosensing applications.","PeriodicalId":13014,"journal":{"name":"IEEE Sensors Letters","volume":"9 7","pages":"1-4"},"PeriodicalIF":2.2000,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Integrated Microfluidic Flexible Test Strip for on-Chip Micromixing and Glucose Sensing With Smartphone-Based Chemiluminescence Platform\",\"authors\":\"Kavita Manekar;Meghana A. Hasamnis\",\"doi\":\"10.1109/LSENS.2025.3572024\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Efficient micromixing and accurate sensing are essential for advancing lab-on-a-chip platforms in biomedical applications. This work introduces a novel microfluidic flexible test strip for on-chip glucose sensing, leveraging a serpentine-shaped microchannel optimized through design and simulation in a software tool to enhance passive mixing of two different fluid samples. The integrated system utilizes a smartphone-based chemiluminescence (CL) detection approach for real-time glucose quantification. A 3D-printed device ensures precise camera alignment for optimal luminescence capture. The polydimethylsiloxane-based microfluidic test strip enables rapid and cost-effective glucose detection, serving as a portable alternative to conventional sensors. Experimental validation demonstrates 97.47% mixing efficiency, ensuring uniform reaction conditions. The system exhibits a strong linear response between 3 and 7 mM, with a 0.3-mM detection limit. CL intensity correlates positively with glucose concentration, following the linear regression, while expected vs. estimated glucose values yield an <italic>R</i><sup>2</sup> of 0.94, confirming high accuracy. These findings highlight the potential of microfluidic-assisted flexible test strips for point-of-care diagnostics, paving the way for advanced biosensing applications.\",\"PeriodicalId\":13014,\"journal\":{\"name\":\"IEEE Sensors Letters\",\"volume\":\"9 7\",\"pages\":\"1-4\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-03-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"IEEE Sensors Letters\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://ieeexplore.ieee.org/document/11007486/\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ENGINEERING, ELECTRICAL & ELECTRONIC\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"IEEE Sensors Letters","FirstCategoryId":"1085","ListUrlMain":"https://ieeexplore.ieee.org/document/11007486/","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
Integrated Microfluidic Flexible Test Strip for on-Chip Micromixing and Glucose Sensing With Smartphone-Based Chemiluminescence Platform
Efficient micromixing and accurate sensing are essential for advancing lab-on-a-chip platforms in biomedical applications. This work introduces a novel microfluidic flexible test strip for on-chip glucose sensing, leveraging a serpentine-shaped microchannel optimized through design and simulation in a software tool to enhance passive mixing of two different fluid samples. The integrated system utilizes a smartphone-based chemiluminescence (CL) detection approach for real-time glucose quantification. A 3D-printed device ensures precise camera alignment for optimal luminescence capture. The polydimethylsiloxane-based microfluidic test strip enables rapid and cost-effective glucose detection, serving as a portable alternative to conventional sensors. Experimental validation demonstrates 97.47% mixing efficiency, ensuring uniform reaction conditions. The system exhibits a strong linear response between 3 and 7 mM, with a 0.3-mM detection limit. CL intensity correlates positively with glucose concentration, following the linear regression, while expected vs. estimated glucose values yield an R2 of 0.94, confirming high accuracy. These findings highlight the potential of microfluidic-assisted flexible test strips for point-of-care diagnostics, paving the way for advanced biosensing applications.