基于智能手机的化学发光平台集成微流控柔性试纸条用于片上微混合和葡萄糖传感

IF 2.2 Q3 ENGINEERING, ELECTRICAL & ELECTRONIC
Kavita Manekar;Meghana A. Hasamnis
{"title":"基于智能手机的化学发光平台集成微流控柔性试纸条用于片上微混合和葡萄糖传感","authors":"Kavita Manekar;Meghana A. Hasamnis","doi":"10.1109/LSENS.2025.3572024","DOIUrl":null,"url":null,"abstract":"Efficient micromixing and accurate sensing are essential for advancing lab-on-a-chip platforms in biomedical applications. This work introduces a novel microfluidic flexible test strip for on-chip glucose sensing, leveraging a serpentine-shaped microchannel optimized through design and simulation in a software tool to enhance passive mixing of two different fluid samples. The integrated system utilizes a smartphone-based chemiluminescence (CL) detection approach for real-time glucose quantification. A 3D-printed device ensures precise camera alignment for optimal luminescence capture. The polydimethylsiloxane-based microfluidic test strip enables rapid and cost-effective glucose detection, serving as a portable alternative to conventional sensors. Experimental validation demonstrates 97.47% mixing efficiency, ensuring uniform reaction conditions. The system exhibits a strong linear response between 3 and 7 mM, with a 0.3-mM detection limit. CL intensity correlates positively with glucose concentration, following the linear regression, while expected vs. estimated glucose values yield an <italic>R</i><sup>2</sup> of 0.94, confirming high accuracy. These findings highlight the potential of microfluidic-assisted flexible test strips for point-of-care diagnostics, paving the way for advanced biosensing applications.","PeriodicalId":13014,"journal":{"name":"IEEE Sensors Letters","volume":"9 7","pages":"1-4"},"PeriodicalIF":2.2000,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Integrated Microfluidic Flexible Test Strip for on-Chip Micromixing and Glucose Sensing With Smartphone-Based Chemiluminescence Platform\",\"authors\":\"Kavita Manekar;Meghana A. Hasamnis\",\"doi\":\"10.1109/LSENS.2025.3572024\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Efficient micromixing and accurate sensing are essential for advancing lab-on-a-chip platforms in biomedical applications. This work introduces a novel microfluidic flexible test strip for on-chip glucose sensing, leveraging a serpentine-shaped microchannel optimized through design and simulation in a software tool to enhance passive mixing of two different fluid samples. The integrated system utilizes a smartphone-based chemiluminescence (CL) detection approach for real-time glucose quantification. A 3D-printed device ensures precise camera alignment for optimal luminescence capture. The polydimethylsiloxane-based microfluidic test strip enables rapid and cost-effective glucose detection, serving as a portable alternative to conventional sensors. Experimental validation demonstrates 97.47% mixing efficiency, ensuring uniform reaction conditions. The system exhibits a strong linear response between 3 and 7 mM, with a 0.3-mM detection limit. CL intensity correlates positively with glucose concentration, following the linear regression, while expected vs. estimated glucose values yield an <italic>R</i><sup>2</sup> of 0.94, confirming high accuracy. These findings highlight the potential of microfluidic-assisted flexible test strips for point-of-care diagnostics, paving the way for advanced biosensing applications.\",\"PeriodicalId\":13014,\"journal\":{\"name\":\"IEEE Sensors Letters\",\"volume\":\"9 7\",\"pages\":\"1-4\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-03-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"IEEE Sensors Letters\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://ieeexplore.ieee.org/document/11007486/\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ENGINEERING, ELECTRICAL & ELECTRONIC\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"IEEE Sensors Letters","FirstCategoryId":"1085","ListUrlMain":"https://ieeexplore.ieee.org/document/11007486/","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
引用次数: 0

摘要

高效的微混合和精确的传感对于推进生物医学应用中的芯片实验室平台至关重要。本研究介绍了一种用于片上葡萄糖传感的新型微流体柔性测试条,利用通过软件工具设计和模拟优化的蛇形微通道来增强两种不同流体样品的被动混合。该集成系统采用基于智能手机的化学发光(CL)检测方法进行实时葡萄糖定量。3d打印设备确保精确的相机对准,以获得最佳的发光捕获。基于聚二甲基硅氧烷的微流体测试条能够快速且经济高效地检测葡萄糖,可作为传统传感器的便携式替代品。实验验证,混合效率为97.47%,反应条件均匀。该系统在3 ~ 7mm范围内具有较强的线性响应,检测限为0.3 mM。根据线性回归,CL强度与葡萄糖浓度呈正相关,而预期葡萄糖值与估计葡萄糖值的R2为0.94,证实了较高的准确性。这些发现突出了微流体辅助柔性试纸在即时诊断方面的潜力,为先进的生物传感应用铺平了道路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Integrated Microfluidic Flexible Test Strip for on-Chip Micromixing and Glucose Sensing With Smartphone-Based Chemiluminescence Platform
Efficient micromixing and accurate sensing are essential for advancing lab-on-a-chip platforms in biomedical applications. This work introduces a novel microfluidic flexible test strip for on-chip glucose sensing, leveraging a serpentine-shaped microchannel optimized through design and simulation in a software tool to enhance passive mixing of two different fluid samples. The integrated system utilizes a smartphone-based chemiluminescence (CL) detection approach for real-time glucose quantification. A 3D-printed device ensures precise camera alignment for optimal luminescence capture. The polydimethylsiloxane-based microfluidic test strip enables rapid and cost-effective glucose detection, serving as a portable alternative to conventional sensors. Experimental validation demonstrates 97.47% mixing efficiency, ensuring uniform reaction conditions. The system exhibits a strong linear response between 3 and 7 mM, with a 0.3-mM detection limit. CL intensity correlates positively with glucose concentration, following the linear regression, while expected vs. estimated glucose values yield an R2 of 0.94, confirming high accuracy. These findings highlight the potential of microfluidic-assisted flexible test strips for point-of-care diagnostics, paving the way for advanced biosensing applications.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
IEEE Sensors Letters
IEEE Sensors Letters Engineering-Electrical and Electronic Engineering
CiteScore
3.50
自引率
7.10%
发文量
194
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信