{"title":"用海藻酸钠-阿拉伯胶复合物制备omega-3脂肪酸纳米胶囊:体外消化率和生物相容性治疗潜力","authors":"Naorem Rojita Devi, Sital Khandelwal, Srinivasan Pappu","doi":"10.1016/j.kjs.2025.100450","DOIUrl":null,"url":null,"abstract":"<div><div>Omega-3 fatty acids (ω3FAs) possess various health benefits but face challenges due to limited solubility and susceptibility to oxidation. In this study, a sodium alginate-acacia gum (SA-AG) complex was developed to address these issues. The stability of the complex was confirmed through zeta potential and thermogravimetric analysis. ω3FAs were encapsulated within the SA-AG complex, and their physical, morphological, and chemical characteristics were evaluated. Simulated <em>in vitro</em> digestion was used to assess the bio-accessibility of the encapsulated ω3FAs (Enω3FAs), and antioxidant, antibacterial, and toxicity tests were conducted. The SA-AG complex exhibited a zeta potential of −40.3 ± 9.48 mV and remained stable up to 240 °C. The oxidative stability of the complex was maintained for up to 90 days post-formulation. The average particle size was 961.9 nm, with spherical structures observed. Energy Dispersive X-ray Analysis (EDAX) showed the presence of ω3FAs at specific peaks (0.05, 0.5, and 1–1.2 KeV), while Fourier Transform Infrared (FT-IR) analysis confirmed the functional groups of ω3FAs at specific bands (3301 cm<sup>−1</sup> and 1630 cm<sup>−1</sup>). Enω3FAs exhibited rapid release of ω3FAs under gastrointestinal conditions, starting at 45 min with 40 % lipolysis. The highest antioxidant activities of ω3FAs were observed at a concentration of 2 mg/ml through DPPH (35 ± 3.8 %), ferric reducing antioxidant power (48.85 ± 0.002 %) at 5.12 mg/mL, 20.71 ± 0.95 nmol of nitric oxide at 5.12 mg/mL, and 0.204 ± 0.001 absorbance for lipid peroxidation at 5.12 mg/mL. The minimum inhibitory concentration of Enω3FAs was 64 mg/ml against <em>E. coli</em>, <em>S. pneumoniae</em>, and <em>P. aeruginosa</em>, and 16 mg/mL against <em>S. aureus</em>. Enω3FAs also demonstrated the ability to reduce biofilm formation by approximately 50 % and induce a 30 % reduction in existing biofilms at a concentration of 4 mg/ml. Additionally, Enω3FAs were found to be biocompatible, with 0 % mortality observed after 24 h of treatment on <em>Artemia franciscana</em> nauplii. In conclusion, Enω3FAs show therapeutic potential within a stable formulation using the SA-AG complex, providing a promising approach for drug and food delivery systems.</div></div>","PeriodicalId":17848,"journal":{"name":"Kuwait Journal of Science","volume":"52 4","pages":"Article 100450"},"PeriodicalIF":1.1000,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Fabrication of omega-3 fatty acid nanoencapsulation with a sodium alginate-acacia gum complex: In vitro digestibility and biocompatible therapeutic potential\",\"authors\":\"Naorem Rojita Devi, Sital Khandelwal, Srinivasan Pappu\",\"doi\":\"10.1016/j.kjs.2025.100450\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Omega-3 fatty acids (ω3FAs) possess various health benefits but face challenges due to limited solubility and susceptibility to oxidation. In this study, a sodium alginate-acacia gum (SA-AG) complex was developed to address these issues. The stability of the complex was confirmed through zeta potential and thermogravimetric analysis. ω3FAs were encapsulated within the SA-AG complex, and their physical, morphological, and chemical characteristics were evaluated. Simulated <em>in vitro</em> digestion was used to assess the bio-accessibility of the encapsulated ω3FAs (Enω3FAs), and antioxidant, antibacterial, and toxicity tests were conducted. The SA-AG complex exhibited a zeta potential of −40.3 ± 9.48 mV and remained stable up to 240 °C. The oxidative stability of the complex was maintained for up to 90 days post-formulation. The average particle size was 961.9 nm, with spherical structures observed. Energy Dispersive X-ray Analysis (EDAX) showed the presence of ω3FAs at specific peaks (0.05, 0.5, and 1–1.2 KeV), while Fourier Transform Infrared (FT-IR) analysis confirmed the functional groups of ω3FAs at specific bands (3301 cm<sup>−1</sup> and 1630 cm<sup>−1</sup>). Enω3FAs exhibited rapid release of ω3FAs under gastrointestinal conditions, starting at 45 min with 40 % lipolysis. The highest antioxidant activities of ω3FAs were observed at a concentration of 2 mg/ml through DPPH (35 ± 3.8 %), ferric reducing antioxidant power (48.85 ± 0.002 %) at 5.12 mg/mL, 20.71 ± 0.95 nmol of nitric oxide at 5.12 mg/mL, and 0.204 ± 0.001 absorbance for lipid peroxidation at 5.12 mg/mL. The minimum inhibitory concentration of Enω3FAs was 64 mg/ml against <em>E. coli</em>, <em>S. pneumoniae</em>, and <em>P. aeruginosa</em>, and 16 mg/mL against <em>S. aureus</em>. Enω3FAs also demonstrated the ability to reduce biofilm formation by approximately 50 % and induce a 30 % reduction in existing biofilms at a concentration of 4 mg/ml. Additionally, Enω3FAs were found to be biocompatible, with 0 % mortality observed after 24 h of treatment on <em>Artemia franciscana</em> nauplii. In conclusion, Enω3FAs show therapeutic potential within a stable formulation using the SA-AG complex, providing a promising approach for drug and food delivery systems.</div></div>\",\"PeriodicalId\":17848,\"journal\":{\"name\":\"Kuwait Journal of Science\",\"volume\":\"52 4\",\"pages\":\"Article 100450\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2025-06-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Kuwait Journal of Science\",\"FirstCategoryId\":\"103\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S230741082500094X\",\"RegionNum\":4,\"RegionCategory\":\"综合性期刊\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MULTIDISCIPLINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Kuwait Journal of Science","FirstCategoryId":"103","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S230741082500094X","RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
Fabrication of omega-3 fatty acid nanoencapsulation with a sodium alginate-acacia gum complex: In vitro digestibility and biocompatible therapeutic potential
Omega-3 fatty acids (ω3FAs) possess various health benefits but face challenges due to limited solubility and susceptibility to oxidation. In this study, a sodium alginate-acacia gum (SA-AG) complex was developed to address these issues. The stability of the complex was confirmed through zeta potential and thermogravimetric analysis. ω3FAs were encapsulated within the SA-AG complex, and their physical, morphological, and chemical characteristics were evaluated. Simulated in vitro digestion was used to assess the bio-accessibility of the encapsulated ω3FAs (Enω3FAs), and antioxidant, antibacterial, and toxicity tests were conducted. The SA-AG complex exhibited a zeta potential of −40.3 ± 9.48 mV and remained stable up to 240 °C. The oxidative stability of the complex was maintained for up to 90 days post-formulation. The average particle size was 961.9 nm, with spherical structures observed. Energy Dispersive X-ray Analysis (EDAX) showed the presence of ω3FAs at specific peaks (0.05, 0.5, and 1–1.2 KeV), while Fourier Transform Infrared (FT-IR) analysis confirmed the functional groups of ω3FAs at specific bands (3301 cm−1 and 1630 cm−1). Enω3FAs exhibited rapid release of ω3FAs under gastrointestinal conditions, starting at 45 min with 40 % lipolysis. The highest antioxidant activities of ω3FAs were observed at a concentration of 2 mg/ml through DPPH (35 ± 3.8 %), ferric reducing antioxidant power (48.85 ± 0.002 %) at 5.12 mg/mL, 20.71 ± 0.95 nmol of nitric oxide at 5.12 mg/mL, and 0.204 ± 0.001 absorbance for lipid peroxidation at 5.12 mg/mL. The minimum inhibitory concentration of Enω3FAs was 64 mg/ml against E. coli, S. pneumoniae, and P. aeruginosa, and 16 mg/mL against S. aureus. Enω3FAs also demonstrated the ability to reduce biofilm formation by approximately 50 % and induce a 30 % reduction in existing biofilms at a concentration of 4 mg/ml. Additionally, Enω3FAs were found to be biocompatible, with 0 % mortality observed after 24 h of treatment on Artemia franciscana nauplii. In conclusion, Enω3FAs show therapeutic potential within a stable formulation using the SA-AG complex, providing a promising approach for drug and food delivery systems.
期刊介绍:
Kuwait Journal of Science (KJS) is indexed and abstracted by major publishing houses such as Chemical Abstract, Science Citation Index, Current contents, Mathematics Abstract, Micribiological Abstracts etc. KJS publishes peer-review articles in various fields of Science including Mathematics, Computer Science, Physics, Statistics, Biology, Chemistry and Earth & Environmental Sciences. In addition, it also aims to bring the results of scientific research carried out under a variety of intellectual traditions and organizations to the attention of specialized scholarly readership. As such, the publisher expects the submission of original manuscripts which contain analysis and solutions about important theoretical, empirical and normative issues.