胸腺瘤组织中鞘磷脂、神经酰胺、甘油磷脂和二酰基甘油水平的改变

IF 3.9 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2025-06-18 DOI:10.1016/j.bbalip.2025.159650

Jie Zhang , Xiaoling Zang , Wei Meng , Peng Jiao , Jiangyu Wu , Lizhen Zhao , Zhuangzhuang Li , Xin Zhang , Huanhuan Yang , Zhihua Lv

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引用次数: 0

摘要

胸腺瘤是一种罕见的胸腺上皮性肿瘤，其发病机制和脂质特征尚不清楚。本研究采用超高效液相色谱-高分辨率质谱法（UPLC-HRMS）进行非靶向脂质组学研究，分析了24例胸腺瘤、14例胸腺增生和1例胸腺癌（TC）患者76份组织样本的脂质变化。含36-44碳的鞘磷脂（SM d36:1, SM d38:1, SM d40:1, SM d36:2, SM d38:2, SM d40:2, SM d41:2, SM d43:2, SM d44:2），中链、长链和超长链神经酰胺（Cer d17:1 16:0, Cer d18:1 17:0, Cer d18:1 18:0, Cer d18:1 22:0, Cer d18:1 24:1, Cer d18:2 20:0, Cer d18:2 22:0），磷脂酰胆碱（PC） 16:0 -16:0，磷脂酰乙醇胺（PE 16:0 -16:0, PE 16:0 -16:0 - 16:1, PE p -16:0 - 18:0 - 18:2）， LPE 18:2，磷脂酰丝氨酸（PS） 18:1_22:0，多不饱和溶血磷脂酰胆碱（lpc）、长或超长多不饱和酰基链的溶血磷脂酰胆碱（PC 16:0_18:3、PC 18:0_22:5、PC 18:1_22:6、PC 15:0_18:2、PC 18:0_20:3、PC 18:0_20:4、PC 18:2_20:4、PC 20:2_20:4、PC 18:0_22:4、PC O-18:0_20:4）、部分已鉴定的PE （PE P-16:0_18:1、PE P-18:0_20:4、PE p -16:0 _22:4、PE P-16:0_22:6）、DG 18:0_20:4、肉碱。与配对的非癌组织相比，胸腺瘤和TC组织中的1-甲基组胺水平升高。胸腺瘤组织中变化最大的途径是甘油磷脂代谢和鞘脂代谢。此外，基于5种脂质（PC 18:0_20:3、PE 16:0_16:0、PC 18:0_22:5、PE P-16:0_18:1、PC O-18:0_20:4）的正交偏最小二乘判别分析（oPLS-DA）将胸腺瘤和1 TC组织与非癌组织区分开来，准确率为97.4%，灵敏度为100%，特异性为95.5%，具有较好的判别能力。

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Altered levels of sphingomyelins, ceramides, glycerophospholipids, and diacylglycerols in thymoma tissues

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Altered levels of sphingomyelins, ceramides, glycerophospholipids, and diacylglycerols in thymoma tissues

Thymoma is a rare thymic epithelial tumor, and its pathogenesis and lipid characteristics are still unclear. In this study, non-targeted lipidomics study by ultra-performance liquid chromatography high-resolution mass spectrometry (UPLC-HRMS) was conducted to analyze the alterations of lipids in 76 tissue samples from 24 thymoma, 14 thymic hyperplasia, and 1 thymic carcinoma (TC) patients. Sphingomyelins with 36–44 carbons (SM d36:1, SM d38:1, SM d40:1, SM d36:2, SM d38:2, SM d40:2, SM d41:2, SM d43:2, SM d44:2), medium, long and very-long chain ceramides (Cer d17:1/16:0, Cer d18:1/17:0, Cer d18:1/18:0, Cer d18:1/22:0, Cer d18:1/24:1, Cer d18:2/20:0, Cer d18:2/22:0), phosphatidylcholine (PC) 16:0_16:0, phosphatidylethanolamines (PE 16:0_16:0, PE 16:0_16:1, PE P-16:0_18:2), LPE 18:2, phosphatidylserine (PS) 18:1_22:0, diacylglycerols (DG 16:0_18:1 and DG 18:1_18:2) showed decreased levels, while polyunsaturated lysophosphatidylcholines (LPCs), PCs with long or very-long polyunsaturated acyl chains (PC 16:0_18:3, PC 18:0_22:5, PC 18:1_22:6, PC 15:0_18:2, PC 18:0_20:3, PC 18:0_20:4, PC 18:2_20:4, PC 20:2_20:4, PC 18:0_22:4, PC 20:4_22:4, PC O-18:0_20:4), part of identified PEs (PE P-16:0_18:1, PE P-18:0_20:4, PE P-16:0_22:6), DG 18:0_20:4, arachidyl carnitine, and 1-methylhistamine had increased levels in thymoma and 1 TC tissues compared to paired non-cancerous tissues. The most altered pathways in thymoma tissues were glycerophospholipid metabolism and sphingolipid metabolism. In addition, orthogonal partial least squares-discriminant analysis (oPLS-DA) based on 5 lipids (PC 18:0_20:3, PE 16:0_16:0, PC 18:0_22:5, PE P-16:0_18:1, and PC O-18:0_20:4) discriminated thymoma and 1 TC tissues from non-cancerous ones with 97.4 % accuracy, 100 % sensitivity, and 95.5 % specificity, showing a good discrimination ability.

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来源期刊

Biochimica et biophysica acta. Molecular and cell biology of lipids 生物-生化与分子生物学

CiteScore

11.00

自引率

2.10%

发文量

109

审稿时长

53 days

期刊介绍： BBA Molecular and Cell Biology of Lipids publishes papers on original research dealing with novel aspects of molecular genetics related to the lipidome, the biosynthesis of lipids, the role of lipids in cells and whole organisms, the regulation of lipid metabolism and function, and lipidomics in all organisms. Manuscripts should significantly advance the understanding of the molecular mechanisms underlying biological processes in which lipids are involved. Papers detailing novel methodology must report significant biochemical, molecular, or functional insight in the area of lipids.