PathoSift Pro：一种新型的基于尺寸的生物气溶胶采样器，用于有效检测传染性空气传播的流感病毒

IF 2.4 3区医学 Q3 VIROLOGY

Virology Pub Date : 2025-06-18 DOI:10.1016/j.virol.2025.110609

Xuan-Dung Nguyen , Bac Le , Qiongying Yang , Binbin Wang , Xiu-Feng Wan

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引用次数: 0

摘要

了解空气传播病毒的动力学需要精确的工具来收集和分析传染性气溶胶。我们介绍了PathoSift Pro，一种新型生物气溶胶采样器，用于有效地收集和保存空气中存活的病原体。pathsift Pro以25 L/min的流速运行，最大限度地减少了脱水和再雾化，克服了Andersen冲击器等传统采样器的局限性。以甲型H1N1流感病毒为模型，我们的研究表明，在模拟环境中，PathoSift Pro有效地捕获了载病毒颗粒的大小分布，比Andersen撞击器多维持了74.76%的活病毒。为了进一步评估其现场适用性，我们通过猪模型实验对PathoSift Pro进行了测试。结果表明，猪的气溶胶大小分布与人类相似，在气溶胶（≤5 μm）和飞沫（>5 μm）颗粒中都检测到活病毒，支持猪作为流感传播研究的转化模型。总之，pathsift Pro为推进空气传播病原体的研究和阐明空气传播动力学提供了有效的工具。

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PathoSift Pro: A novel size-based bioaerosol sampler for effectively detecting infectious airborne influenza virus

Understanding the dynamics of airborne virus transmission requires precise tools for collecting and analyzing infectious aerosols. We present PathoSift Pro, a novel bioaerosol sampler engineered to efficiently collect and preserve viable airborne pathogens. Operating at a flow rate of 25 L/min, PathoSift Pro minimizes dehydration and re-aerosolization, overcoming the limitations of conventional samplers like the Andersen impactor. Using H1N1 influenza A virus as a model, our study demonstrated that PathoSift Pro effectively captured size distributions of virus-laden particles in simulated settings and maintained 74.76 % more viable viruses than the Andersen impactor. To further evaluate its field applicability, we tested PathoSift Pro through experiments using a pig model. Results showed that pigs exhibited aerosol size distributions similar to humans, with viable viruses detected in both aerosol (≤5 μm) and droplet (>5 μm) particles, supporting pigs as a translational model for influenza transmission studies. In summary, PathoSift Pro provides an effective tool for advancing research on airborne pathogens and elucidating airborne transmission dynamics.

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来源期刊

Virology 医学-病毒学

CiteScore

6.00

自引率

0.00%

发文量

157

审稿时长

50 days

期刊介绍： Launched in 1955, Virology is a broad and inclusive journal that welcomes submissions on all aspects of virology including plant, animal, microbial and human viruses. The journal publishes basic research as well as pre-clinical and clinical studies of vaccines, anti-viral drugs and their development, anti-viral therapies, and computational studies of virus infections. Any submission that is of broad interest to the community of virologists/vaccinologists and reporting scientifically accurate and valuable research will be considered for publication, including negative findings and multidisciplinary work.Virology is open to reviews, research manuscripts, short communication, registered reports as well as follow-up manuscripts.